Journal of Plant Research

, Volume 109, Issue 4, pp 415–424

Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea

  • Satoshi Iuchi
  • Kazuko Yamaguchi-Shinozaki
  • Takeshi Urao
  • Kazuo Shinozaki
Original Articles

Abstract

Ten cDNAs for drought-inducible genes were isolated using differential screening of a cDNA library prepared from 10-hr dehydrated cowpea plants,Vigna unguiculata (S. Iuchi, K. Yamaguchi-Shinozaki, T. Urao, T. Terao, K. Shinozaki; Plant Cell Physiology, 1996 in press). Two of the cDNA clones, designated CPRD12 and CPRD46, were sequenced and characterized. The CPRD12 and CPRD46 cDNAs encode putative proteins related to nonmetallo-short-chain alcohol dehydrogenase (CPRD12) and chloroplastic lipoxygenase (CPRD46). Northern blot analysis revealed that these genes are induced by high-salinity stress and exogenous abscisic acid, but not by cold stress. The CPRD46 gene is also responsive to heat stress and methyl jasmonate and salicylic acid. Genomic Southern blot analysis suggested that CPRD12 constitutes a small gene family, but that CPRD46 is a single copy gene. We discuss the possible functions of these two CPRD gene products under drought stress.

Key words

Abscisic acid Cowpea (Vigna unguiculataDrought Gene Expression Lipoxygenase Nonmetallo-short-chain alcohol dehydrogenase 

Abbreviations

ABA

abscisic acid

ADH

alcohol dehydrogenase

JA

jasmonic acid

meJA

methyl JA

SA

salicylic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bell, E., Creelman, R.A. andMullet, J.E. 1995. A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis. Proc. Natl. Acad. Sci. USA92: 8675–8679.PubMedGoogle Scholar
  2. Bell, E. andMullet, J.E. 1993. Characterization of an Arabidopsis lipoxygenase gene responsive to methyl jasmonate and wounding. Plant Physiol.103: 1133–1137.CrossRefPubMedGoogle Scholar
  3. Blechert, S., Brodschelm, W., Holder, S., Kammerer, L., Kutchan, T.M., Mueller, M.J., Xia, Z.Q. andZenk, M.H. 1995. The octadecanoic pathway: signal molecules for the regulation of secondary pathways. Proc. Natl. Acad. Sci. USA92: 4099–4105.PubMedGoogle Scholar
  4. Bohnert, H.J., Nelson, D.E. andJensen, R.G. 1995. Adaptations to environmental stresses. Plant Cell7: 1099–1111.CrossRefPubMedGoogle Scholar
  5. Bruxelles, G.L. de, Peacock, W.J., Dennis, E.S. andDolferus, R. 1996. Abscisic acid induces the alcohol dehydrogenase gene inArabidopsis. Plant Physiol.111: 381–391.PubMedGoogle Scholar
  6. DeLong, A., Calderon-Urrea, A. andDellaporta, S.L. 1993. Sex determination geneTASSELSEED2 of maize encodes a short-chain alcohol dehydrogenase required for stage-specific floral organ abortion. Cell74: 757–768.CrossRefPubMedGoogle Scholar
  7. Dolferus, R., Jacobs, M., Peacock, W.J. andDennis, E.S. 1994. Differential interactions of promoter elements in stress responses of theArabidopsis Adh gene. Plant Physiol.105: 1075–1087.CrossRefPubMedGoogle Scholar
  8. Ealing, P.M. andCasey, R. 1988. The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA. Biochem. J.253: 915–918.PubMedGoogle Scholar
  9. Ealing, P.M. andCasey, R. 1989. The cDNA cloning of a pea (Pisum sativum) seed lipoxygenase. Sequence comparisons of the two major pea seed lipoxygenase isoforms. Biochem. J.264: 929–932.PubMedGoogle Scholar
  10. Eiben, H.G. andSlusarenko, A.J. 1994. Complex spatial and temporal expression of lipoxygenase genes duringPhaseolus vulgaris (L.) development. Plant J.5: 123–135.PubMedGoogle Scholar
  11. Enyedi, A.J., Yalpani, N., Silverman, P. andRaskin, I. 1992. Signal molecules in systemic plant resistance to pathogens and pests. Cell70: 879–886.CrossRefPubMedGoogle Scholar
  12. Heijne, G. von andNishikawa, K. 1991. Chloroplast transit peptides. The perfect random coil? FEBS Lett.278: 1–3.Google Scholar
  13. Ingram, J. andBartels, D. 1996. The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.47: 377–403.CrossRefPubMedGoogle Scholar
  14. Iuchi, S., Yamaguchi-Shinozaki, K., Urao, T., Terao, T. and Shinozaki, K. 1996. Novel drought-inducible genes in the highly drought-tolerant cowpea: cloning of cDNAs and analysis of their gene expression. Plant Cell Physiol. in press.Google Scholar
  15. Jacobsen, S.E. andOlszewski, N.E. 1996. Gibberellins regulate the abundance of RNAs with sequence similarity to proteinase inhibitors, dioxygenases and dehydrogenases. Planta198: 78–86.CrossRefPubMedGoogle Scholar
  16. Jany, K.-D., Ulmer, W., Froeschie, M. andPfleiderer, G. 1984. Complete amino acid sequence of glucose dehydrogenase fromBacillus megaterium. FEBS Lett.165: 6–10.CrossRefPubMedGoogle Scholar
  17. Kawaoka, A., Kawamoto, T., Sekine, M., Yoshida, K., Takano, M. andShinmyo, A. 1994. A cis-acting element and a trans-acting factor involved in the wound-induced expression of a horseradish peroxidase gene. Plant J.6: 87–97.CrossRefPubMedGoogle Scholar
  18. Malamy, J., Carr, J.P., Klessig, D.F. andRaskin, I. 1990. Salicylic acid: a likely endogenous signal in the resistance response of tobacco to viral infection. Science250: 1002.Google Scholar
  19. Meier, B.M., Shaw, N. andSlusarenko, A.J. 1993. Spatial and temporal accumulation of defense gene transcripts in bean (Phaseolus vulgaris) leaves in relation to bacteria-induced hypersensitive cell death. Mol. Plant Microbe Interact.6: 453–66.PubMedGoogle Scholar
  20. Melan, M.A., Dong, X., Eudara, M.E., Davis, K.R., Ausubel, F.M. andPeterman, T.K. 1993. AnArabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant Physiol.101: 441–450.CrossRefPubMedGoogle Scholar
  21. Nagy, F., Kay, S.A. andChua, N.-H. 1988. Analysis of gene expression in transgenic plants.In: S.V., Gelvin and R.A. Schilperoort, eds., Plant Molecular Biology Manual, B4. pp. 1–29. Kluwer Academic Publishers, Dordrecht.Google Scholar
  22. Ohta, H., Shirano, Y., Tanaka, K., Morita, Y. andShibata, D. 1992. cDNA cloning of rice lipoxygenase L-2 and characterization using active enzyme expressed from the cDNA inEscherichia coli. Eur. J. Biochem.206: 331–336.PubMedGoogle Scholar
  23. Peng, Y.L., Shirano, Y., Ohta, H., Hibino, T., Tanaka, K. andShibata, D. 1994. A novel lipoxygenase from rice. Primary structure and specific expression upon incompatible infection with rice blast fungus. J. Biol. Chem.269: 3755–3761.PubMedGoogle Scholar
  24. Persson, B., Krook, M. andJornvall H. 1991. Characteristics of short-chain alcohol dehydrogenases and related enzymes. Eur. J. Biochem.200: 537–543.PubMedGoogle Scholar
  25. Reinbothe, S., Mollenhauer, B. andReinbothe, C. 1994. JIPs and RIPs: the regulation of plant gene expression by jasmonates in response to environmental cues and pathogens. Plant Cell6: 1197–1209.CrossRefPubMedGoogle Scholar
  26. Sambrook, J., Fritsch, E.F. andManiatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
  27. Sembdner, G. andParthler, B. 1993. The biochemistry and the physiological and molecular actions of jasmonates. Annu. Rev. Plant Physiol. Plant Mol. Biol.44: 569–589.CrossRefGoogle Scholar
  28. Shibata, D. andAxelrod, B. 1995. Plant lipoxygenases. J. Lipid Mediators Cell Signalling12: 213–228.Google Scholar
  29. Shibata, D., Kato, T. andTanaka, K. 1991. Nucleotide sequences of a soybean lipoxygenase gene and the short intergenic region between an upstream lipoxygenase gene. Plant Mol. Biol.16: 353–359.CrossRefPubMedGoogle Scholar
  30. Shibata, D., Slusarenko, A., Casey, R., Hildebrand, D. andBell, E. 1994. Lipoxygenase. Plant Mol. Biol. Rep.12: S41-S42.Google Scholar
  31. Shibata, D., Steczko, J., Dixon, J.E., Andrews, P.C., Hermodson, M. andAxelrod, B. 1988. Primary structure of soybean lipoxygenase L-2. J. Biol. Chem.263: 6816–6821.PubMedGoogle Scholar
  32. Shibata, D., Steczko, J., Dixon, J.E., Hermodson, M., Yazdanparast, R. andAxelrod, B. 1987. Primary structure of soybean lipoxygenase-1. J. Biol. Chem.262: 10080–10085.PubMedGoogle Scholar
  33. Shinozaki, K. andYamaguchi-Shinozaki, K. 1996. Molecular responses to drought and cold stress. Cur. Opinion Biotech.7: 161–167.Google Scholar
  34. Singh, B.B. 1993. The cowpea breeding section of the archival report (1988–1992) of the grain legume improvement program. International Institute of Tropical Agriculture, Ipadan, Nigeria.Google Scholar
  35. Steczko, J., Donoho, G.P., Clemens, J.C., Dixon, J.E. andAxelrod, B. 1992. Conserved histidine residues in soybean lipoxygenase: functional consequences of their replacement. Biochemistry31: 4053–4057.CrossRefPubMedGoogle Scholar
  36. Summerfield, R.J., Pate, J.S., Roberts, E.H. andWien, H.C. 1985. The physiology of cowpeas.In S.R. Singh and K.O. Rachie, eds., Cowpea Research, Production and Utilization. pp. 65–101. John Wiley & Sons, Chichester, U.K.Google Scholar
  37. Yamaguchi-Shinozaki, K., Urao, T. andShinozaki, K. 1995. Regulation of genes that are induced by drought stress inArabidopsis thaliana. J. Plant Res.108: 127–136.CrossRefGoogle Scholar
  38. Yenofsky, R.L., Fine, M. andLiu, C. 1988. Isolation and characterization of a soybean (Glycine max) lipoxygenase-3 gene. Mol. Gen. Genet.211: 215–222.CrossRefGoogle Scholar
  39. Zhang, Y.Y., Radmark, O. andSamuelsson, B. 1992. Mutagenesis of some conserved residues in human 5-lipoxygenase: effects on enzyme activity. Proc. Natl. Acad. Sci. USA89: 485–489.PubMedGoogle Scholar

Copyright information

© The Botanical Society of Japan 1996

Authors and Affiliations

  • Satoshi Iuchi
    • 2
    • 3
  • Kazuko Yamaguchi-Shinozaki
    • 1
  • Takeshi Urao
    • 1
  • Kazuo Shinozaki
    • 2
    • 3
  1. 1.Biological Resources DivisionJapan International Research Center for Agricultural Sciences (JIRCAS), Ministry of Agriculture, Forestry, and FisheriesTsukuba, IbarakiJapan
  2. 2.Laboratory of Plant Molecular BiologyThe Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science CenterTsukuba, IbarakiJapan
  3. 3.Institute of Biological ScienceTsukuba UniversityTsukuba, IbarakiJapan

Personalised recommendations