Journal of Plant Research

, Volume 109, Issue 4, pp 415–424

Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea

  • Satoshi Iuchi
  • Kazuko Yamaguchi-Shinozaki
  • Takeshi Urao
  • Kazuo Shinozaki
Original Articles


Ten cDNAs for drought-inducible genes were isolated using differential screening of a cDNA library prepared from 10-hr dehydrated cowpea plants,Vigna unguiculata (S. Iuchi, K. Yamaguchi-Shinozaki, T. Urao, T. Terao, K. Shinozaki; Plant Cell Physiology, 1996 in press). Two of the cDNA clones, designated CPRD12 and CPRD46, were sequenced and characterized. The CPRD12 and CPRD46 cDNAs encode putative proteins related to nonmetallo-short-chain alcohol dehydrogenase (CPRD12) and chloroplastic lipoxygenase (CPRD46). Northern blot analysis revealed that these genes are induced by high-salinity stress and exogenous abscisic acid, but not by cold stress. The CPRD46 gene is also responsive to heat stress and methyl jasmonate and salicylic acid. Genomic Southern blot analysis suggested that CPRD12 constitutes a small gene family, but that CPRD46 is a single copy gene. We discuss the possible functions of these two CPRD gene products under drought stress.

Key words

Abscisic acid Cowpea (Vigna unguiculataDrought Gene Expression Lipoxygenase Nonmetallo-short-chain alcohol dehydrogenase 



abscisic acid


alcohol dehydrogenase


jasmonic acid


methyl JA


salicylic acid


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Copyright information

© The Botanical Society of Japan 1996

Authors and Affiliations

  • Satoshi Iuchi
    • 2
    • 3
  • Kazuko Yamaguchi-Shinozaki
    • 1
  • Takeshi Urao
    • 1
  • Kazuo Shinozaki
    • 2
    • 3
  1. 1.Biological Resources DivisionJapan International Research Center for Agricultural Sciences (JIRCAS), Ministry of Agriculture, Forestry, and FisheriesTsukuba, IbarakiJapan
  2. 2.Laboratory of Plant Molecular BiologyThe Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science CenterTsukuba, IbarakiJapan
  3. 3.Institute of Biological ScienceTsukuba UniversityTsukuba, IbarakiJapan

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