Theoretical and Applied Genetics

, Volume 60, Issue 4, pp 197–214 | Cite as

Somaclonal variation — a novel source of variability from cell cultures for plant improvement

  • P. J. Larkin
  • W. R. Scowcroft


It is concluded from a review of the literature that plant cell culture itself generates genetic variability (somaclonal variation). Extensive examples are discussed of such variation in culture subclones and in regenerated plants (somaclones). A number of possible mechanisms for the origin of this phenomenon are considered. It is argued that this variation already is proving to be of significance for plant improvement. In particular the phenomenon may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species. It may also be used to generate variants of a commercial cultivar in high frequency without hybridizing to other genotypes.

Key words

Plant cell culture Somaclones Interspecific hybrids Gene amplification Virus elimination 


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  1. Abo, El-Nil; Hildebrandt, A.C. (1972): Morphological changes in geranium plants differentiated from anther cultures. In vitro7, 258 (abstr.)Google Scholar
  2. Ahloowalia, B.S. (1976): Chromosomal changes in parasexually produced rye grass. In: Current Chromosome Research (eds.: Jones, K.; Brandham, P.E.), pp. 115–122. Amsterdam: ElsevierGoogle Scholar
  3. Ahloowalia, B.S. (1978): Novel ryegrass genotypes regenerated from embryo-callus culture. Fourth Intl. Congr. Plant Tissue Cell Culture, (abst.) pp. 162, Calgary, Canada, 1978Google Scholar
  4. Arcia, M.A.; Wernsman, E.A.; Burk, L.G. (1978): Performance of anther-derived dihaploids and their conventionally inbred parent as lines, in F1 hybrids, and in F2 generations. Crop Sci.18, 413–418Google Scholar
  5. Barbier, M.; Dulieu, H.L. (1980): Effets génétiques observés sur des plantes de Tabac régénérées à partir de cotylédons par culture in vitro. Ann. Amélior. Plant.30, 321–344Google Scholar
  6. Bayliss, M.W. (1973): Origin of chromosome number variation in cultured plant cells. Nature246, 529–530Google Scholar
  7. Bayliss, M.W. (1980): Chromosomal variation in plant tissues in culture. Intern. Rev. Cytol. Suppl.11B, 113–144Google Scholar
  8. Beach, K.H.; Smith, R.R. (1979): Plant regeneration from callus of red clover and crimson clover. Plant Sci. Lett.16, 231–238Google Scholar
  9. Behnke, M. (1979): Selection of potato callus for resistance to culture filtrates ofPhytophthora infestans and regeneration of resistant plants. Theor. Appl. Genet.55, 69–71PubMedGoogle Scholar
  10. Behnke, M. (1980): General resistance to late blight ofSolanum tuberosum plants regenerated from callus resistant to culture filtrates ofPhytophthora infestans. Theor. Appl. Genet.56, 151–152PubMedGoogle Scholar
  11. Ben-Jaacov, J.; Langhans, R.W. (1972): Rapid multiplication ofChrysanthemum plants by stem-tip proliferation. Hort. Sci.7, 289–290Google Scholar
  12. Bennici, A. (1974): Cytological analysis of roots, shoots and plants regenerated from suspension and solid in vitro cultures of haploidPelargonium.Z. Pflanzenzücht.72, 199–205Google Scholar
  13. Bingham, E.T.; Hurley, L.V.; Kaatz, D.M.; Saunders, J.W. (1975): Breeding alfalfa which regenerates from callus tissue in culture. Crop Sci.15, 719–721Google Scholar
  14. Blakely, L.M.; Steward, F.C. (1964): Growth and organized development of cultured cells. 7 Cellular variation. Am. J. Bot.51, 809–820Google Scholar
  15. Bourgin, J.P. (1978): Valine-resistant plants from in vitro selected tobacco cells. Molec. Gen. Genet.161, 225–230Google Scholar
  16. Brack, C.; Hirama, M.; Lehnard-Schueller, R.; Tonegawa, S. (1978): A complete immunoglobin gene is created by somatic recombination. Cell15, 1–4PubMedGoogle Scholar
  17. Brettell, R.I.S.; Ingram, D.S. (1979): Tissue culture in the production of novel disease-resistant crop plants. Biol. Rev.54, 329–345Google Scholar
  18. Brettell, R.I.S.; Thomas, R.; Ingram, D.S. (1980): Reversion of Texas male-sterile cytoplasm maize in culture to give fertile, Ttoxin resistant plants. Theor. Appl. Genet.58, 55–58Google Scholar
  19. Bukhari, A.I.; Shapiro, J.A.; Adhya, S.L. (1977): Eds: DNA Insertion Elements, Plasmids and Episomes. New York: Cold Spring Harbor LaboratoryGoogle Scholar
  20. Burk, L.G.; Chaplin, J.F. (1980): Variation among anther-derived haploids from a multiple disease-resistant tobacco hybrid. Crop Sci.20, 334–338Google Scholar
  21. Burk, L.G.; Matzinger, D.F. (1976): Variation among anther-derived doubled haploids from an inbred line of tobacco. J. Hered.67, 381–384Google Scholar
  22. Burk, L.G.; Chaplin, J.F.; Gooding, G.V.; Powell, N.T. (1979): Quantity production of anther-derived haploids from a multiple disease resistant tobacco hybrid. 1. Frequency of plants with resistance or susceptibility to tobacco mosaic virus (TMV), potato virus (PVY) and root knot (RK). Euphytica28, 201–208Google Scholar
  23. Calos, M.P.; Miller, J.H. (1980): Transposable elements. Cell20, 579–595PubMedGoogle Scholar
  24. Cameron, J.R.; Loh, E.Y.; Davis, R.W. (1979): Evidence for transposition of dispersed repetitive DNA families in yeast. Cell16, 739–752PubMedGoogle Scholar
  25. Carlson, P. (1973): Methionine sulfoximine-resistant mutants of tobacco. Science180, 1366–1368PubMedGoogle Scholar
  26. Catcheside, D.G. (1947): The P-locus position effect in Oenothera J. Genet.48, 31–42PubMedGoogle Scholar
  27. Chaleff, D.T.; Fink, G.R. (1980): Genetic events associated with an insertion mutation in yeast. Cell21, 227–237PubMedGoogle Scholar
  28. Chaleff, R.S.; Parsons, M.F. (1978a): Direct selection in vitro for herbicide — resistant mutants ofNicotiana tabacum. Proc. Nat. Acad. Sci. (USA)75, 5704–5707Google Scholar
  29. Chaleff, R.S.; Parsons, M.F. (1978b): Isolation of a glycerol-utilizing mutant ofNicotiana tabacum. Genetics89, 723–728PubMedCentralPubMedGoogle Scholar
  30. Chaturvedi, H.C.; Mitra, G.C. (1975): A shift in morphogenetic pattern inCitrus callus tissue during prolonged culture. Ann. Bot.39, 683–687Google Scholar
  31. Collins, G.B.; Legg, P.D. (1980): Recent advances in the genetic applications of haploidy inNicotiana. In: The Plant Genome (eds.: Davies, D.R.; Hopwood, D.A.), pp. 197–213. Norwich: The John Innes CharityGoogle Scholar
  32. Crisp, P.; Walkey, D.G.A. (1974): The use of aseptic meristem culture in cauliflower breeding. Euphytica23, 305–313Google Scholar
  33. Cullis, C.A. (1973): DNA differences between flax genotrophs. Nature243, 515–516PubMedGoogle Scholar
  34. Cullis, C.A. (1975): Environmentally induced DNA changes in flax. In: Modification of the Information Content of Plant Cells (eds. Markham R. et al.), pp. 27–36. North-Holland: ElsevierGoogle Scholar
  35. Cullis, C.A.; Goldsborough, P.B. (1980): Variation in the ribosomal RNA gene number — a model system for DNA changes in flax genotrophs. In: The Plant Genome (eds. Davies, D.R.; Hopwood, D.A.), pp. 91–97. Norwich: The John Innes CharityGoogle Scholar
  36. Cummings, D.P.; Green, C.E.; Stuthman, D.D. (1976): Callus induction and plant regeneration in oats. Crop Sci.16, 465–470Google Scholar
  37. Davey, M.R.; Fowler, M.W.; Street, H.E. (1971): Cell clones contrasted in growth, morphology and pigmentation isolated from a callus culture ofAtropa belladonna var ‘lutea’. Phytochemistry10, 2559–2575Google Scholar
  38. D'Amato, F. (1978): Chromosome number variation in cultured cells and regenerated plants. In: Frontiers of Plant Tissue Culture 1978 (ed. Thorpe, T.A.), pp. 287–295. Calgary: Intern. Ass. Plant Tissue CultureGoogle Scholar
  39. Deambrogio, E.; Dale, P.J. (1980): Effect of 2,4-D on the frequency of regenerated plants in barley and on genetic variability between them. Cereal Res. Comm.8, 417–423Google Scholar
  40. De Marsac, N.T.; Jouanneau, J.P. (1972): Variation de l'exigence en cytokinine de lignées clonales de cellules de tabac. Physiol. Vég.10, 369–380Google Scholar
  41. De Paepe, R. (1980): Genetic variation amongst haploid doubled plants obtained by androgenesis inNicotiana sylvestris. In: The Plant Genome (eds. Davies, D.R.; Hopwood, D.A.), p. 245. Norwich: The John Innes CharityGoogle Scholar
  42. Devreux, M.; Laneri, V. (1974): Anther culture, haploid plants, isogenic line and breeding research inNicotiana tabacum L. In: Polyploidy and Induced Mutations in Plant Breeding, pp. 101–107, Vienna: IAEAGoogle Scholar
  43. Dickinson, D.G.; Baker, R.F. (1978): Evidence for translocation of DNA sequences during sea urchin embryogenesis. Proc. Nat. Acad. Sci. (USA)75, 5627–7630Google Scholar
  44. Doolittle, W.F.; Sapienza, C. (1980): Selfish genes, the phenotype paradigm and genome evolution. Nature284, 601–603PubMedGoogle Scholar
  45. dos Santos, A.V.P.; Outka, D.E.; Cocking, E.C.; Davey, M.R. (1980): Organogenesis and somatic embryogenesis in tissues derived from leaf protoplasts and leaf explants ofMedicago sativa. Z. Pflanzenphysiol.99, 261–270Google Scholar
  46. Evans, D.A.; Paddock, E.F. (1976): Comparisons of somatic crossing over frequency inNicotiana tabacum and three other crop species. Can. J. Genet. Cytol.18, 57–65Google Scholar
  47. Evans, D.A.; Paddock, E.F. (1980): Characterization of X-ray induced increase of mitotic cross-overs inGlycine max. Theor. Appl. Genet.56, 245–251PubMedGoogle Scholar
  48. Fincham, J.R.S.; Sastry, G.R.K. (1974): Controlling elements in maize. Ann. Rev. Genet.8, 15–50PubMedGoogle Scholar
  49. Finnegan, D.J.; Rubin, G.M.; Young, M.W.; Hogness, D.S. (1978): Repeated gene families inDrosophila melanogaster. Cold Spring Harbor Symp. Quant. Biol.42, 1053–1063PubMedGoogle Scholar
  50. Flavell, R.B. (1975): Quantitative Variation in nucleolar ribosomal RNA gene multiplicity in wheat and rye. In: Modification of the Information Content of Plant Cells (eds. Markham, R. et al.), pp. 53–62. Amsterdam: ElsevierGoogle Scholar
  51. Foroughi-Wehr, B.; Mix, G.; Friedt, W. (1979): Fertility of microspore derived plants over three successive generations. Barley Genet. Newslett.9, 20–22Google Scholar
  52. Gamborg, O.L.; Shyluk, J.P.; Brar, D.S.; Constabel, F. (1977): Morphogenesis and plant regeneration from callus of immature embryos of sorghum. Plant Sci. Lett.10, 67–74Google Scholar
  53. Gautheret, R.J. (1955): The nutrition of plant tissue cultures. Ann. Rev. Plant Physiol.6, 433–484Google Scholar
  54. Gengenbach, B.G.; Green, C.E. (1975): Selection of T-cytoplasm maize callus cultures resistant toHelminthosporium maydis Race T pathotoxin. Crop Sci.15, 645–649Google Scholar
  55. Gengenbach, B.G.; Green C.E.; Donovan, C.M. (1977): Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc. Nat. Acad. Sci. (USA)74, 5113–5117Google Scholar
  56. Gerstel, D.U.; Burns, J.A.; Burk, L.G. (1974): Meiosis in F1 hybrids between haploid-derived diploids and their parents. Tob. Sci.18, 122–124Google Scholar
  57. Green, G.E. (1977): Prospects for crop improvement in the field of cell culture. Hortsci.12, 7–10Google Scholar
  58. Green, C.E. (1978): In vitro plant regeneration in cereals and grasses. In: Frontiers of Plant Tissue Culture 1978 (ed.: Thorpe, T.A.), pp. 411–418. Calgary: Int. Ass. Plant Tissue CultureGoogle Scholar
  59. Green, C.E.; Phillips, R.L.; Wang, A.S. (1977): Cytological analysis of plants regenerated from maize tissue cultures. Maize Genet. Newslett.51, 53–54Google Scholar
  60. Green, M.M. (1977): The case for DNA insertion mutations in Drosophila. In: DNA Insertion Elements, Plasmids and Episomes (eds.: Bukhari, A.I.; Shapiro, J.A.; Adhya, S.L.), pp. 437–455. New York: Cold Spring Harbor LaboratoryGoogle Scholar
  61. Grout, B.W.W.; Crisp, P. (1980): The origin and nature of shoots propagated from cauliflower roots. J. Hortic. Sci.55, 65–70Google Scholar
  62. Hacket, W.P.; Anderson, J.M. (1967): Asceptic multiplication and maintenance of differentiated carnation shoot tissue derived from shoot apices. Proc. Am. Soc. Hort. Sci.90, 365–369Google Scholar
  63. Hagemann, R. (1958): Somatische konversion beiLycopersicon esculentum Mill. Z. Vererbungsl.89, 587–613PubMedGoogle Scholar
  64. Heinz, D.J. (1973): Sugar-cane improvement through induced mutations using vegetative propagules and cell culture techniques. In: Induced Mutations in Vegetatively Propagated Plants (Proc. of a Panel, 11–15 September 1972, pp. 53–59. Vienna: Intern. Atomic Energy AgencyGoogle Scholar
  65. Heinz, D.J. (1976): Tissue culture in breeding. Annl. Rep. Hawaiian Sugar Planters Assoc. Expt. Stations, pp. 9–11Google Scholar
  66. Heinz, D.J.; Mee, G.W.P. (1969): Plant differentiation from callus tissue ofSaccharum species. Crop. Sci.9, 346–348Google Scholar
  67. Heinz, D.J.; Mee, G.W.P.; Nickell, L.G. (1969): Chromosome numbers of someSaccharum species hybrids and their cell suspension cultures. Am. J. Bot.56, 450–456Google Scholar
  68. Heinz, D.J.; Mee, G.W.P. (1971): Morphologic, cytogenetic, and enzymatic variation inSaccharum species hybrid clones derived from callus tissue. Am. J. Bot.58, 257–262Google Scholar
  69. Heinz, D.J.; Krishnamurthi, M.; Nickell, L.G.; Maretzki, A. (1977): Cell, tissue and organ culture in sugarcane improvement. In: Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture (eds.: Reinert, J.; Bajaj, Y.P.S.), pp. 3–17. Berlin: SpringerGoogle Scholar
  70. Henke, R.R.; Mansur, M.A.; Constantin, M.J.(1978): Organogenesis and plantlet formation from organ- and seedling-derived calli of rice (Oryza sativa). Physiol. Plant.44, 11–14Google Scholar
  71. Hibberd, K.A.; Walter, T.; Green, C.E.; Gengenbach, B.G. (1980): Selection and characterization of a feedback-insensitive tissue culture of maize. Planta148, 183–187PubMedGoogle Scholar
  72. Hoffmann, F. (1978): Mutation and selection of haploid cell culture systems of rape and rye. In: Production of Natural Compounds by Cell Culture Methods (eds.: Alfermann, A.W.; Reinhard, E.), pp. 319–329. München: Gesellschaft für Strahlen- und UmweltforschungGoogle Scholar
  73. Horãk, J.; Landa, Z.; Lustinec, J. (1971): Production of polyploid plants from tissue cultures ofBrassica oleracea L. Phyton28, 7–10Google Scholar
  74. Horák, J.; Lustinec, J.; Mesícek, J.; Kamínek, M.; Polácková, D. (1975): Regeneration of diploid and polyploid plants from the stem with expiants of diploid narrow stem kale (Brassica oleracea L). Ann. Bot.3, 571–577Google Scholar
  75. Ibrahim, R.K. (1969): Normal and abnormal plants from carrot root tissue cultures. Can. J. Bot.47, 825–826Google Scholar
  76. Jackson, P.J. (1980): Characterization of the ribosomal DNA of soybean cells. Fed. Proc.39, 1878Google Scholar
  77. Jelaska, S.; Papes, D.; Pevalek, B.; Devide, Z. (1978): Developmental and karyological studies ofVicia faba callus cultures,p. 101, (abst), Fourth Intl. Cong. Plant Tissue Cell Cult., Calgary, CanadaGoogle Scholar
  78. Kado, C.I.; Kleinhofs, A. (1980): Genetic modification of plant cells through uptake of foreign DNA. Intern. Rev. Cytol. Suppl.11B, 47–80Google Scholar
  79. Kao, K.W.; Michayluk, M.R. (1980): Plant regeneration from mesophyll protoplasts of alfalfa. Z. Pflanzenphysiol.96, 135–141Google Scholar
  80. Kao, K.W.; Miller, R.N.; Gamborg, O.L.; Harvey, B.L. (1970): Variations in chromosome number and structure in plant cells grown in suspension cultures. Can. J. Genet. Cytol.12, 297–301Google Scholar
  81. Kasperbauer, M.J.; Buckner, R.C.; Bush, L.P. (1979): Tissue culture of annual ryegrass × tall fescue F1 hybrids: callus establishment and plant regeneration. Crop. Sci.19, 457–460Google Scholar
  82. Kassanis, B.; White, R.F.; Woods, R.D. (1977): Beet cryptic virus. Phytopathol. Z.90, 350–360Google Scholar
  83. Kleckner, N. (1977): Translocatable elements in procaryotes. Cell11, 11–23PubMedGoogle Scholar
  84. Krishnamurthi, M. (1974): Notes on disease resistance of tissue culture sub-clones and fusion of sugar cane protoplasts. Sugarcane Breeder's Newslett.35, 24–26Google Scholar
  85. Krishnamurthi, M.; Tlaskal, J. (1974): Fiji disease resistantSaccharum officinarum var Pindar subclones from tissue cultures. Proc. Int. Soc. Sugar Cane. Technol.15, 130–137Google Scholar
  86. Lat, J.B.; Lantin, M.M. (1976): Agronomic performance of sugar cane clones derived from callus tissue. Philippine J. Crop Sci.1, 117–123Google Scholar
  87. Latch, G.C.M.; Potter, L.R. (1977): Interaction between crown rust (Puccinia coronata) and two viruses of ryegrass. Ann. appl. Biol.87, 139–145Google Scholar
  88. Larkin, P.J.; Scowcroft, W.R. (1981): Eyespot disease of sugar cane — host-specific toxin induction and its interaction with leaf cells. Plant Physiol.67, 408–414PubMedCentralPubMedGoogle Scholar
  89. Lawyer, A.L.; Berlyn, M.B.; Zelitch, I. (1980): Isolation and characterization of glycine hydroxamate resistant cell lines ofNicotiana tabacum. Plant Physiol.66, 334–341PubMedCentralPubMedGoogle Scholar
  90. Levings, C.S. III; Kim, B.D.; Pring, D.R.; Conde, M.F.; Mans, R.J.; Laughnan, J.R.; Gabay-Laughran, S.J. (1980): Cytoplasmic reversion of cms-S in maize: association with a transpositional event. Science209, 1021–1023PubMedGoogle Scholar
  91. Lewis, E.B. (1950): The phenomenon of position effect. Adv. Genet.3, 73–116PubMedGoogle Scholar
  92. Liu, M-C.; Chen, W-H. (1976): Tissue and cell culture as aids to sugarcane breeding. 1. Creation of genetic variation through callus culture. Euphytica25, 393–403Google Scholar
  93. Liu, M-C.; Chen, W-H. (1978a): Tissue and cell culture as aids to sugar cane breeding. 2. Performance and yield potential of callus derived lines. Euphytica27, 273–282Google Scholar
  94. Liu, M-C.; Chen, W-H. (1978b): Improvement in sugar cane by using tissue culture methods, p. 163. (abst) Fourth Intl. Congr. Plant Tissue Cell Culture, Calgary, Canada.Google Scholar
  95. Liu, M-C.; Huang, Y-J.; Shih, S-C (1972): The in vitro production of plants from several tissues ofSaccharum species. J. Agric. Ass. China New Series77, 52–58Google Scholar
  96. Liu, M-C.; Shang, K.C., Chen, W-H.; Shih, S.C. (1977): Tissue and cell culture as aids to sugarcane breeding. Aneuploid cells and plants induced by treatment of cell suspension cultures with colchicine. Proc. XVI Cong. Int. Soc. Sugar cane Technol. 1, 29–41Google Scholar
  97. McClintock, B. (1956): Controlling elements and the gene. Cold Spring Harbor Symp. Quant. Biol.21, 197–216PubMedGoogle Scholar
  98. McCoy, T. (1979): PhD thesis, University of MinnesotaGoogle Scholar
  99. Coy, T.J.; Phillips, R.L.; Cummings, D.P. (1978): Cytogenetic variability in plants regenerated from tissue cultures of oats (Avena sativa), p. 101, (abst), Fourth Intl. Congr. Plant Tissue Cell Culture, Calgary, CanadaGoogle Scholar
  100. Maliga, P. (1978): Resistance mutants and their use in genetic manipulation. In: Frontiers of Plant Tissue Culture 1978 (ed.: Thorpe, T.A.), pp. 381–392. Calgary: Int. Ass. Plant Tissue CultureGoogle Scholar
  101. Malnassy, P.; Ellison, J.H. (1970): Asparagus tetraploids from callus tissue. Hortsci.5, 444–445Google Scholar
  102. Márton, L.; Maliga, P. (1975): Control of resistance in tobacco cells to 5-bromodeoxyuridine by a simple mendelian factor. Plant. Sci. Lett.5, 77–82Google Scholar
  103. Matern, U.; Strobel, G.; Shepard, J. (1978): Reaction to phytotoxins in a potato population derived from mesophyll protoplasts. Proc. Nat. Acad. Sci. (USA)75, 4935–4939Google Scholar
  104. Meins, F.; Lutz, J. (1979): Tissue-specific variation in the cytokinin habituation of cultured tobacco cells. Differentiation15, 1–6Google Scholar
  105. Meins, F.; Lutz, J.; Binns, A.N. (1980): Variation in the competence of tobacco pith cells for cytokinin-habituation in culture. Differentiation16, 71–75Google Scholar
  106. Mitzukami, H.; Konoshima, M.; Tabata, M. (1978): Variation in pigment production inLithospermum erythorrhizon callus cultures. Phytochemistry17, 95–97Google Scholar
  107. Molgaard, H.V. (1980): Assembly of immunoglobulin heavy chain genes. Nature286, 657–659PubMedGoogle Scholar
  108. Morel, G. (1971): The impact of plant tissue culture on plant breeding. In: The Way Ahead in Plant Breeding. (eds.: Lupton, F.G.H.; Jenkins, G.; Johnson, R.), pp. 185–194. Cambridge: 6 Congr. EucarpiaGoogle Scholar
  109. Mousseau, J. (1970): Fluctuations induites par la néoformation de bourgeons in vitro. In: Cultures des Tissues de Plantes. Strasbourg Coll. Intern. CNRS293, 234–239Google Scholar
  110. Müller, A.J.; Grafe, R. (1978): Isolation and characterization of cell lines ofNicotiana tabacum lacking nitrate reductase. Molec. Gen. Genet.161, 67–76Google Scholar
  111. Murashige, T. (1974): Plant propagation through tissue culture. Ann. Rev. Plant Physiol.25, 135–166Google Scholar
  112. Murashige, T. (1978): The impact of plant tissue culture on agriculture. In: Frontiers of Plant Tissue Culture 1978 (ed.: Thorpe, T.A.), pp. 15–26. Calgary: Int. Ass. Plant Tissue CultureGoogle Scholar
  113. Murashige, T.; Nakano, R. (1967): Chromosome complement as a determinant of the morphogenic potential of tobacco cells. Am. J. Bot.54, 963–970Google Scholar
  114. Nabors, M.W.; Gibbs, S.E.; Bernstein, C.S.; and Meis, M.E. (1980): NaCl-tolerant plants from cultured cells. Z. Pflanzenphysiol.97, 13–17Google Scholar
  115. Nagl, W. (1979): Differential DNA replication in plants: A critical review. Z. Pflanzenphysiol.95, 283–314Google Scholar
  116. Nickell, L.G.; Heinz, D.J. (1973): Potential of cell and tissue culture techniques as aids in economic plant improvement. In: Genes, Enzymes, and Populations (ed.: Srb, A.M.), pp. 109–128. New York: PlenumGoogle Scholar
  117. Nitzsche, W.; Wenzel, G. (1977): Haploids in plant breeding. Berlin, Hamburg: PareyGoogle Scholar
  118. Nishi, T.; Yamada, Y.; Takahashi, E. (1968): Organ redifferentiation and plant restoration in rice callus. Nature219, 508–509PubMedGoogle Scholar
  119. Novák, F.J. (1980): Phenotype and cytological status of plants regenerated from callus cultures ofAllium sativum L. Z. Pflanzenzücht.84, 250–260Google Scholar
  120. Oinuma, T.; Yoshida, T. (1974): Genetic variation among doubled haploid lines of burley tobacco varieties. Jpn. J. Breed.24, 211–216Google Scholar
  121. Oono, K. (1975): Production of haploid plants of rice (Oryza sativa) by anther culture and their use for breeding. Bull. Nat. Inst. Agric. Sci.D26, 139–222Google Scholar
  122. Oono, K. (1978a): High frequency mutations in rice plants regenerated from seed callus. pp. 52, (abst). Fourth Int. Congr. Plant Tissue Cell Culture, Calgary, CanadaGoogle Scholar
  123. Oono, K. (1978b): Test tube breeding of rice by tissue culture. Trop. Agric. Res. Series11, 109–123Google Scholar
  124. Orgel, L.E.; Crick, F.H.C. (1980): Selfish DNA — the ultimate parasite. Nature284, 604–607PubMedGoogle Scholar
  125. Orton, T.J. (1980a): Chromosomal variability in tissue cultures and regenerated plants ofHordeum. Theor. Appl. Genet.56, 101–112PubMedGoogle Scholar
  126. Orton, T.J. (1980b): Haploid barley regenerated from callus cultures ofHordeum vulgare ×H. jubatum. J. Hered.71, 780–782Google Scholar
  127. Orton, T.J.; Steidl, R.P. (1980): Cytogenetic analysis of plants regenerated from colchicine-treated callus cultures of an inter specificHordeum hybrid. Theor. Appl. Genet.57, 89–95PubMedGoogle Scholar
  128. Peterson, P.A. (1970): Controlling elements and mutable loci in maize: their relationship to bacterial episomes. Genetica41, 33–56PubMedGoogle Scholar
  129. Peterson, P.A.; Weber, C.R. (1969): An unstable locus in soybeans. Theor. Appl. Genet.3, 156–162Google Scholar
  130. Petes, T.D. (1980): Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell19, 765–774PubMedGoogle Scholar
  131. Phillips, G.C.; Collins, G.B. (1979): In vitro tissue culture of selected legumes and plant regeneration from callus cultures of red clover. Crop Sci.19, 59–64Google Scholar
  132. Popchristov, V.D.; Zaganska, N.A. (1977): Study of the seed progeny of regenerated plants obtained by the tissue culture of tobacco. In: Uses of Plant Tissue Cultures in Breeding, pp. 209–221. Prague: Ustav Experimentalni Botaniky CSAVGoogle Scholar
  133. Potrykus, I. (1970): Mutation und Rückmutation extrachromosomal vererbter Plastidenmerkmale vonPetunia. Z. Pflanzenzücht.63, 24–40Google Scholar
  134. Potter, L.R. (1980): The effects of barley yellow dwarf virus and powdery mildew in oats and barley with single and dual infections. Ann. Appl. Biol.94, 11–17Google Scholar
  135. Potter, S.; Truett, M.; Phillips, M.; Maher, A. (1980): Eucaryotic transposable genetic elements with inverted terminal repeats. Cell20, 639–47PubMedGoogle Scholar
  136. Roeder, G.S.; Fink, G.R. (1980): DNA rearrangements associated with a transposable element in yeast. Cell21, 239–249PubMedGoogle Scholar
  137. Roy, S.C. (1980): Chromosomal variations in the callus tissues ofAllium tuberosum andA. cepa. Protoplasma102, 171–176Google Scholar
  138. Reuther, G. (1977). Adventitious organ formation and somatic embryogenesis in callus ofAsparagus andIris and its possible application. Acta Hortic. Scan.78, 217–224Google Scholar
  139. Sacristan, M.D.; Melchers, G. (1969): The caryological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Molec. Gen. Genet.105, 317–333PubMedGoogle Scholar
  140. Saka, H.; Voqui-Dinh, T.N.; Chang, T.Y. (1980): Stimulation of multiple shoot formation on soybean stem nodes in culture. Plant Sci. Lett.19, 193–201Google Scholar
  141. Sand, S. (1976): Genetic control of gene expression: independent location of Flt (3) and its interactions with the mutable V locus inNicotiana. Genetics83, 719–736PubMedCentralPubMedGoogle Scholar
  142. Schimke, R.T.; Alt, F.W.; Kellems, R.E.; Kaufman, N.; Bertino, J.R. (1977): Selective multiplication of dihydrofolate reductase gene in methotrexate-resistant variants of cultured murine cells. Cold Spring Harbor Symp. Quant. Biol.42, 649–657Google Scholar
  143. Schubert, I.; Rieger, R. (1979): Asymmetric banding ofVicia faba chromosomes after BUdR incorporation. Chromosoma70, 385–391Google Scholar
  144. Schubert, I.; Künzel, G.; Bretschneider, H.; Rieger, R. (1980): Sister chromatid exchanges in barley. Theor. Appl. Genet.56, 1–4PubMedGoogle Scholar
  145. Scowcroft, W.R. (1977): Somatic cell genetics and plant improvement. Adv. Agron.29, 39–81Google Scholar
  146. Selby, C.; Collin, H.A. (1976): Clonal variation in growth and flavour production in tissue cultures ofAllium cepa L. Ann. Bot.40, 911–918Google Scholar
  147. Shepard, J.F.; Bidney, D.; Shahin, E. (1980): Potato protoplasts in crop improvement. Science28, 17–24Google Scholar
  148. Shimada, T.; Yamada, Y. (1979): Wheat plants regenerated from embryo cell cultures. Jpn. J. Genet.54, 379–385Google Scholar
  149. Sibi, M. (1976): La notion de programme génétique chez les végétaux supérieurs. II. Aspect expérimental. Obtention de variants par culture de tissues in vitro surLactuca sativa L. apparition de vigueur chez les chroisements. Ann. Amélior. Plant.26, 523–547Google Scholar
  150. Siegel, A. (1975): Gene amplification in plants. In: Modification of the Information Content of Plant Cells (eds.: Markham, R. et al.), pp. 15–26. Amsterdam: ElsevierGoogle Scholar
  151. Sievert, R.C.; Hildebrandt, A.C. (1965): Variations within single cell clones of tobacco tissue cultures. Am. J. Bot.52, 742–750PubMedGoogle Scholar
  152. Siminovitch, L. (1976): On the nature of hereditable variation in cultured somatic cells. Cell7, 1–11PubMedGoogle Scholar
  153. Simmonds, N.W. (1969): Prospects of potato improvement. Scottish Plant Breed. Stat. Ann. Report 1968–69, pp. 18–38Google Scholar
  154. Skirvin, R.M. (1978): Natural and induced variation in tissue culture. Euphytica27, 241–266Google Scholar
  155. Skirvin, R.M., Janick, J. (1967a): Tissue culture-induced variation in scentedPelargonium spp. J. Amer. Soc. Hort. Sci.101, 281–290Google Scholar
  156. Skirvin, R.M.; Janick, J. (1976b): ‘Velvet Rose’Pelargonium, a scented geranium. HortSci.11, 61–62Google Scholar
  157. Snijman, D.A.; Noel, A.R.A.; Bornman, C.H.; Abbott, J.G. (1977):Nicotiana tabacum callus studies. 2. Variability in culture. Z. Pflanzenphysiol.82, 367–370Google Scholar
  158. Spiegel-Roy, P.; Kochba, J. (1975): Production of solid mutants in citrus, utilizing new approaches and techniques. In: Improvement of Vegetatively Propagated Plants Through Induced mutations, Tokai, 1974, pp. 113–117. Vienna: Intern. Atomic Energy AgencyGoogle Scholar
  159. Spofford, J.B. (1976): Position-effect variegation inDrosophila. In: The Genetics and Biology ofDrosophila, Vol 1c (eds. Ashburner, M.; Novitski, E.), pp. 955–1018. London: Acad. PressGoogle Scholar
  160. Sree Ramulu, K.; Devreux, M.; Ancora, G.; Laneri, U. (1976): Chimerism inLycopersicon peruvianum plants regenerated from in vitro cultures of anthers and stem internodes. Z. Pflanzenzücht.76, 299–319Google Scholar
  161. Starlinger, P. (1980): A reexamination of McClintock's ‘Controlling Elements’ in maize in view of recent advances in molecular biology. In: Genome Organization and Expression in Plants. (ed. Leaver, C.J.), pp. 537–551. New York: PlenumGoogle Scholar
  162. Stimart, D.P.; Ascher, P.D.; Zagorski, J.S. (1980): Plants from callus of the interspecific hybridLilium ‘Black Beauty’. HortScience15, 313–315Google Scholar
  163. Strickland, R.G.; Sunderland, N. (1972): Production of anthocyanins, flavonols and chlorogenic acid production in cultured callus ofHaplopappus gracilis. Ann. Bot.36, 443–457Google Scholar
  164. Strobel, E.; Dunsmuir, P.; Rubin, G.M. (1979): Polymorphism in the chromosomal locations of elements of the 412,copia and 297 dispersed repeated gene families inDrosophila. Cell17, 429–439PubMedGoogle Scholar
  165. Strom, C.M.; Moscona, M.; Dorfman, A. (1978): Amplification of DNA sequences during chicken cartilage and neural retina differentiation. Proc. Nat. Acad. Sci. (USA)75, 4459–4462Google Scholar
  166. Sung, Z.R.; Jacques, S. (1980): 5-Fluorouracil resistance in carrot cell cultures. Its use in studying the interaction of the pyrimidine and arginine pathways. Planta148, 389–396PubMedGoogle Scholar
  167. Tabata, M.; Hiraoka, N. (1976): Variation of alkaloid production inNicotiana rustica callus cultures. Physiol. Plant.38, 19–23Google Scholar
  168. Tabata, M.; Ogino, T.; Yoshioka, K.; Yoshikawa, N.; Hiraoka, N. (1978): Selection of cell lines with higher yield of secondary products. In: Frontiers of Plant Tissue Culture 1978 (ed.: Thorpe, T.A.), pp. 213–222. Calgary: Ass. Plant Tissue CultureGoogle Scholar
  169. Tartof, K.D. (1973): Unequal sister chromatid exchange and disproportionate replication as mechanisms regulating ribosomal RNA gene redundancy. Cold Spring Harbor Symp. Quant. Biol.38, 491–500Google Scholar
  170. Thomas, E.; King, P.J.; Potrykus, I. (1979): Improvement of crop plants via single cells in vitro — an assessment. Z.Pflanzenzücht.82, 1–30Google Scholar
  171. Tokumasu, S.; Kato, M. (1979): Variation of chromosome numbers and essential oil components of plants derived from anther culture of the diploid and tetraploid inPelargonium roseum. Euphytica28, 329–338Google Scholar
  172. Umiel, N.; Brand, E.; Goldner, R. (1978): Streptomycin resistance in tobacco: I. Variation among calliclones in the phenotypic expression of resistance. Z. Pflanzenzücht.88, 311–15Google Scholar
  173. Vazquez, A.M.; Davey, M.R.; Short, K.C. (1977): Organogenesis in cultures ofSaintpaulia ionantha. Acta Horticult. 78, 249–258Google Scholar
  174. Wahl, G.M.; Padgett, R.A.; Stark, G.R. (1979): Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells. J. Biol. Chem.254, 8679–8689PubMedGoogle Scholar
  175. Wakasa, K. (1979): Variation in the plants differentiated from the tissue culture of pineapple. Jpn. J. Breeding29, 13–22Google Scholar
  176. Weill, J.C.; Reynaud, C.A. (1980): Somatic Darwinism in vivo. Biosystems12, 23–25PubMedGoogle Scholar
  177. Wenzel, G. (1980): Recent progress in microspore culture of crop plants. In: The Plant Genome (eds. Davies, D.R.; Hopwood, D.A.), pp. 185–196. Norwich: The John Innes CharityGoogle Scholar
  178. Wenzel, G.; Schieder, O.; Przewozny, T.; Sopory, S.K.; Melchers, G. (1979): Comparison of single cell culture derivedSolanum tuberosum L. plants and a model for their application in breeding programs. Theor. Appl. Genet.55, 49–55PubMedGoogle Scholar
  179. Widholm, J.M. (1977): Selection and characterization of aminoacid analog resistant plant cell cultures. Crop Sci.17, 597–600Google Scholar
  180. Williams, L.; Collins, H.A. (1976): Growth and cytology of celery plants derived from tissue cultures. Ann. Bot.40, 333–338Google Scholar
  181. Zenk, M.H.; El-Shagi, H.; Arens, H.; Stöckigt, J.; Weiler, E.W.; Deus, B. (1977): Formation of the indole alkaloids sepentine and ajmalicine in cell suspension cultures ofCatharanthus roseus. In: Plant Tissue Culture and Its Bio-technological Application (eds.: Barz, W.; Reinhard, E.; Zenk, M.H.), pp. 27–43. Berlin: SpringerGoogle Scholar
  182. Zenk, M.H. (1978): The impact of plant cell culture on industry. In: Frontiers of Plant Tissue Culture 1978 (ed.: Thorpe, T.A.), pp. 1–13. Calgary: Int. Ass. Plant Tissue CultureGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • P. J. Larkin
    • 1
  • W. R. Scowcroft
    • 1
  1. 1.Division of Plant IndustryCSIROCanberraAustralia

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