Radiation and Environmental Biophysics

, Volume 12, Issue 1, pp 31–39

Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle

  • Heinz Baisch
  • Wolfgang Göhde
  • Walfried A. Linden
Article

DOI: 10.1007/BF02339807

Cite this article as:
Baisch, H., Göhde, W. & Linden, W.A. Radiat Environ Biophys (1975) 12: 31. doi:10.1007/BF02339807
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Summary

Mathematical models for the analysis of pulse-cytophotometric (PCP) data are described. With computer programs based on these models the fractions of cells in G1-, S- and(G2 + M)-phases are obtained. The methods are applied to PCP measurements of Ehrlich ascites tumor cells, human bone marrow cells and L-929-cells in culture. The results of the L-cell experiment are compared with autoradiographic results; for both methods the duration of the various phases has been calculated. Two different mathematical models for PCP-data evaluation and the autoradiographic method yielded results agreeing within statistical error. The application of the two models on different types of DNA-histograms is discussed: One model is suitable for asynchronous cell populations with a low fraction of S-phase cells, the other can be applied for partially synchronized cells and high S-phase fractions as well.

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Heinz Baisch
    • 1
    • 2
  • Wolfgang Göhde
    • 1
    • 2
  • Walfried A. Linden
    • 1
    • 2
  1. 1.Institut für Biophysik und Strahlenbiologie der Universität HamburgHamburg 20Federal Republic of Germany
  2. 2.Institut für Strahlenbiologie der Universität MünsterFederal Republic of Germany

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