Evolution of disintegrin cysteine-rich and mammalian matrix-degrading metalloproteinases: Gene duplication and divergence of a common ancestor rather than convergent evolution
- 92 Downloads
The evolution of the Metalloproteinase Disintegrin Cysteine-rich (MDC) gene family and that of the mammalian Matrix-degrading Metalloproteinases (MMPs) are compared. The alignment of snake venom and mammalian MDC and MMP precursor sequences generated a phylogenetic tree that grouped these proteins mainly according to their function. Based on this observation, a common ancestry is suggested for mammalian and snake venom MDCs; it is also possible that gene duplication of the already-assembled domain structure, followed by divergence of the copies, may have significantly contributed to the evolution of the functionally diverse MDC proteins. The data also suggest that the structural resemblance of the zinc-binding motif of venom MDCs and MMPs may best be explained by common ancestry and conservation of the proteolytic motifs during the divergence of the proteins rather than through convergent evolution.
Key wordsMetalloproteinase Disintegrin Evolution Venom Phylogeny Gene duplication
Unable to display preview. Download preview PDF.
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tools. J Mol Biol 215:403–410Google Scholar
- Blundell TL (1994) Metalloproteinase superfamilies and drug design. Nat Struc Biol 1:73–75Google Scholar
- Creigton TE, Darby NJ (1989) Functional evolutionary divergence of proteolytic enzymes and their inhibitors. Trends Biochem Sci 14: 319–324Google Scholar
- Dayhoff MO, Schartz RM, Orcutt BC (1978) Atlas of protein sequence and structure, vol 5, supplement 3. NBRF, Washington, p 345Google Scholar
- Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791Google Scholar
- Genetics Computer Group (1991) Program manual for the GCG package, Version 7. 575 Science Drive, Madison, WI 53711, USAGoogle Scholar
- Gould RJ, Polokoff MA, Friedman PA, Huang TF, Holt JC, Cook JJ, Niewarowski S (1990) Disintegrins—a family of integrins inhibitory proteins from viper venoms. Proc Sue Exp Biol Med 5:168–171Google Scholar
- Higgins DG, Bleasby AJ, Fuchs R (1992) Cluster V: improved software for multiple sequence alignment. Comput Appl Biosci 2: 189–191Google Scholar
- Wolfsberg TG, Bazan JF, Blobel CP, Myles DG, Primakoff P, White JM (1993) The precursor region of a protein active in sperm-egg fusion contains a metalloproteinase and disintegrin domain: structural, functional, and evolutionary implications. Proc Natl Acad Sci USA 90:10783–10787PubMedGoogle Scholar
- Yoshida S, Setoguchi M, Higuchi Y, Akizuki S, Yamamoto S (1990) Molecular cloning of a cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in routine monocytic lineage. Int Immunol 2: 586–591Google Scholar