Valepotriate content in different in vitro cultures of Valerianaceae and characterization of Valeriana officinalis L. callus during a growth period
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Differentin vitro cultures of Valerianaceae were analysed for valepotriate content [(iso)valtrate, acevaltrate, didrovaltrate] in a study on properties of productionin vitro (plant species, growth conditions, differentiation level, valepotriate content of the medium after growth). Thein vitro cultures were: callus cultures ofValeriana officinalis L., Valerianella locusta L. andCentranthus ruber L.DC.; a suspension culture ofValeriana officinalis L. and a root organ culture ofCentranthus ruber L.DC. All of the cultures produced valepotriatesin vitro in different amounts. None of the media that had served for growth contained any valepotriates.
In order to characterize thein vitro growth more precisely different parameters were analysed at different time intervals during a growth period in one of the cultures (callus culture ofValeriana officinalis L.). These different parameters were: fresh and dry weight, lipid and nitrogen content and (iso)valtrate content. This study during a growth period was performed on two media differing in plant hormone content.
KeywordsNitrogen Lipid Internal Medicine Plant Species Growth Condition
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