Advertisement

The Histochemical Journal

, Volume 28, Issue 8, pp 531–575 | Cite as

Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites andin situ hybridization

  • Frank Dolbeare
Review

Summary

This paper is a continuation of parts I (history, methods and cell kinetics) and II (clinical applications and carcinogenesis) published previously (Dolbeare, 1995Histochem. J.27, 339, 923). Incorporation of bromodeoxyuridine (BrdUrd) into DNA is used to measure proliferation in normal, diseased and injured tissue and to follow the effect of growth factors. Immunochemical detection of BrdUrd can be used to determine proliferative characteristics of differentiating tissues and to obtain birth dates for actual differentiation events. Studies are also described in which BrdUrd is used follow the order of DNA replication in specific chromasomes, DNA replication sites in the nucleus and to monitor DNA repair. BrdUrd incorporation has been used as a tool forin situ hybridization experiments.

Keywords

Growth Factor Clinical Application Diagnostic Tool Differentiation Event Hybridization Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aarnaes, E., Kirkhus, B. &Clausen, O. P. (1990) Mathematical model analysis of mouse epidermal cell kinetics measured by bivariate DNA/anti-bromodeoxyuridine flow cytometry and continous [3H]-thymidine labelling.Cell Tissue Kinet. 23, 409–24.PubMedGoogle Scholar
  2. Aarnaes, E., Clausen, O. P., Kirkhus, B., De Angelis, P. (1993) Heterogeneity in the mouse epidermal cell cycle analysed by computer simulations.Cell Prolif. 26, 205–19.PubMedGoogle Scholar
  3. Abe, Y., Takahashi, J., Fukuda, H. &Yoshida, K. (1992) Cell kinetic study of murine jejunal crypts during multiple doses per day.Tohoku J. Exp. Med. 168, 311–15.PubMedGoogle Scholar
  4. Achary, P. M., Jajumbar, K., Duttagupta, A. &Mukerjee, A. S. (1981) Replication of DNA in larval salivary glands ofDrosophila after in vivo synchronization.Chromasoma 82, 505–14.Google Scholar
  5. Ackemann, M. F., Lamm, K. R., Wiegand, G. W. &Luster, M. I. (1989) Antitumour activity of murine neutrophils demonstrated by cyyometric analysis.Cancer Res. 49, 528–32.Google Scholar
  6. Adriaansen, H. J., Osman, C., Van Dongen, J. J. M., Wijdenes-De Bresser, J. H. F. M., Kappetijn, Van Tilborg C. M. J. M. &Hooijkaas, H. (1990) Immunological marker analysis of mitogen-induced proliferating lymphocytes using BrdU incorporation or screening of metaphases. Staphylococcal protein A is a potent mitogen for CD4+lymphocytes.Scand. J. Immunol. 32, 687–94.PubMedGoogle Scholar
  7. Aghamohammadi, S. Z. &Savage, J. R. (1990) BrdU pulse/reverse staining protocols for investigating chromasome replication.Chromosoma 99, 76–82.PubMedCrossRefGoogle Scholar
  8. Ahmed, F. E. (1980) The combined action of chemical carcinogens on DNA repair in human cells.Radiat. Environ. Biophys. 18, 57–64.PubMedCrossRefGoogle Scholar
  9. Ahmed, F. E. &Setlow, R. B. (1978) Excision repair in ataxia telangiectasia, Fanconi's anemia, Cockayne syndrome, and Bloom's syndrome after treatment with ultraviolet radiation andN-acetoxy-2-acetylaminofluorene.Biochim. Biophys. Acta 521, 805–17.PubMedGoogle Scholar
  10. Akiya, O., Sfrizawa, N., Sughara, M., Katagiri, S. &Kawashima, Y. (1992) A histological study of changes in the lingual papillae of streptozotocin-induced diabetic rats.Bull. Tokyo Dent. Coll. 33, 13–24.PubMedGoogle Scholar
  11. Al-Ghoul, W. M. &Miller, M. W. (1993) Orderly migration of neurons to the principal sensory nucleus of the trigeminal nerve of the rat.J. Comp. Neurol. 330, 464–75.PubMedGoogle Scholar
  12. Allison, L., Arndt-Jovin, D. J., Gratzner, H., Ternynck, T. &Robert-Nicoud, M. (1985) Mapping of the pattern of DNA replication in polytene chromosome fromChironomus thummi using monoclonal anti-bromodeoxyuridine antibodies.Cytometry 6, 584–90.PubMedCrossRefGoogle Scholar
  13. Almazan, G. &Mckay, R. (1992) An oligodendrocyte precursor cell line on rat optic nerve.Brain Res. 579, 234–45.PubMedCrossRefGoogle Scholar
  14. Arcos-Terán, L (1972) Replikation und die Natur der spät replizierenden Orte im X-Chromosom vonDrosophila melanogaster.Chromosoma 37, 233–96.PubMedCrossRefGoogle Scholar
  15. Arimura, A. (1991) [S phase cells of regenerative epithelium in the healing process of gastric ulcer determined by an in vitro BrdU-anti BrdU method].Nippon Shokakibyo Gakkai Zasshi 88, 664–71.PubMedGoogle Scholar
  16. Arola, J., Heikkila, P. &Kahri, A. I. (1993) Biphasic effect of ACTH on growth of rat adrenocortical cells in primary culture.Cell Tissue Res. 271, 169–76.PubMedCrossRefGoogle Scholar
  17. Atkin, S. L., Landolt, A. M., Jeffreys, R. V., Hipkin, L., Radcliffe, J., Squire, C. R. &White, M. C. (1993) Differential effects of insulin-like growth factor 1 on the hormonal product and proliferation of glycoprotein-secreting human pituitary adenomas.J. Clin. Endocrinol. Metab. 77, 1059–66.PubMedGoogle Scholar
  18. Avossa, D. &Pfeiffer, S. E. (1993) Transient reversion of O4+GalC-oligodendrocyte progenitor development in response to the phorbol ester TPA.J. Neurosci. Res. 34, 113–28.PubMedCrossRefGoogle Scholar
  19. Bachem, M. G., Riess, U. &Gressner, A. M. (1989) Liver fat storing cell proliferation is stimulated by epidermal growth factor/transforming growth factor alpha and inhibited by transforming growth factor beta.Biochem. Biophys. Res. Commun. 162, 708–14.PubMedCrossRefGoogle Scholar
  20. Baeza-squiban, A., Romet, S., Moreau, A. &Marano, F. (1991) Progress in outgrowth culture from rabbit tracheal explants: balance between proliferation and maintenance of differentiated state in epithelial cells.In Vitro Cell Dev. Biol. 27A, 453–60.PubMedGoogle Scholar
  21. Balazs, M., Mayall, B. H. &Waldman, F. M. (1991) Interphase cytogenetics of a male breast cancer.Cancer Genet. Cytogenet. 55, 243–7.PubMedCrossRefGoogle Scholar
  22. Baptista, C. A., Gershon, T. R. &Macagno, E. R. (1990) Peripheral organs control central neurogenesis in the leech.Nature 346, 855–7PubMedCrossRefGoogle Scholar
  23. Baron, C. &Penit, C. (1990) Study of the thymocyte cell cycle by bivariate analysis of incorporated bromodeoxyuridine and DNA content.Eur. J. Immunol. 20, 1231–6.PubMedGoogle Scholar
  24. Baumann, P. H., Schormann, T. &Jovin, T. M. (1992) Three-dimensional component labelling of digital confocal microscope images enumerates replication centers in BrdUrd labeled fibroblasts.Cytometry 13, 220–9.PubMedCrossRefGoogle Scholar
  25. Beisker, W. &Hittelman, W. N. (1988) Measurement of the kinetics of DNA repair synthesis after UV irradiation using immunochemical staining of incorporated 5-bromo-2′-deoxyuridine and flow cytometry.Exp. Cell Res. 174, 156–67.PubMedCrossRefGoogle Scholar
  26. Berges, R. R., Furuya, Y., Remington, L., English, H. F., Jacks, T. &Isaacs, J. T. (1993) Cell proliferation, DNA repair, and p53 function are not required for programmed death of prostatic glandular cells induced by androgen ablation.Proc. Natl. Acad. Sci. USA 90, 8910–14.PubMedGoogle Scholar
  27. Bernardini, S., Cannata, S. M. &Filoni, S. (1992) Spinal cord and ganglia regeneration in larvalXenopus laevis following unilateral ablation.J. Hirnforsch 33, 241–8.PubMedGoogle Scholar
  28. Berry, M. &Eayrs, J. T. (1963) Histogenesis of the cerebral cortex.Nature 197, 984–5.Google Scholar
  29. Bessereau, J. L., Fontaine, B. &Changeux, J. P. (1990) Denervation of mouse skeletal muscle differentially affects the expression of the jun and fos proto-oncogenes.New Biol. 2, 375–83.PubMedGoogle Scholar
  30. Betancourt, M., Ortiz, R. &Gonzalez, C. (1992) Proliferation index in bone marrow cells from severely malnourished rats during lactation.Mutat. Res. 283, 173–7.PubMedCrossRefGoogle Scholar
  31. Biasco, G., Paganelli, G. M., Miglioli, M. &Barbara, L. (1992) Cell proliferation biomarkers in the gastrointestinal tract.J. Cell Biochem. (suppl.) 16G, 73–8.Google Scholar
  32. Biffo, S., Verdun Di Cantogno L. &Fasolo, A. (1992) Double labeling with non-isotopic in situ hybridization and BrdU immunohistochemistry: calmodulin (CaM) mRNA expression in post-mitotic neurons of the olfactory system.J. Histochem. Cytochem 40, 535–40.PubMedGoogle Scholar
  33. Bode, H. R., Gee, L. W. &Chow, M. A. (1990) Neuron differentiation in hydra involves dividing intermediates.Dev. Biol. 139, 231–3.PubMedCrossRefGoogle Scholar
  34. Boehme, S. A. &Lenardo, M. J. (1993) Propriocidal apoptosis of mature T lymphocytes occurs at S phase of the cell cycle.Eur. J. Immunol. 23, 1552–60.PubMedGoogle Scholar
  35. Bourrat, F. &Sotelo, C. (1991) Relationships between neuronal birthdates and cytoarchitecture in the rat inferior olivary complex.J. Comp. Neurol. 313, 509–21.PubMedCrossRefGoogle Scholar
  36. Braisted, J. E. &Raymond, P. A. (1992) Regeheration of dopaminergic neurons in goldfish retina.Development,114, 913–19.PubMedGoogle Scholar
  37. Brandriff, B. F. &Gordon, L. A. (1989) Analysis of the first cell cycle in the cross between hamster eggs and human sperm.Gamete Res. 23, 299–308.PubMedCrossRefGoogle Scholar
  38. Brown, D. B. &Stanfield, B. B. (1989) The use of bromodeoxyuridine-immunohistochemistry to identify transplanted fetal brain tissue.J. Neural Transplant 1, 135–9.PubMedGoogle Scholar
  39. Brown, E. H. &Schildkeraut, C. L. (1979) Perturbation of growth and differentiation of Friend murine erythroleukemia cells by 5-bromodeoxyuridine incorporation in early Sphase.J. Cell. Physiol. 99, 261–77.PubMedCrossRefGoogle Scholar
  40. Burr, A. W., Carpenter, M. R., Hines, J. E., Gullick, W. J. &Burt, A. D. (1993) Intrahepatic distribution of transforming growth factor-alpha (TGF alpha) during liver regeneration following carbon tetrachloride-induced necrosis.J. Pathol. 170, 95–100.PubMedCrossRefGoogle Scholar
  41. Bussolati, O., Uggeri, J., Rotoli, B. M., Franchi-Gazzola, R. &Gazzola, G. C. (1993) The relationship between sodiumdependent transport of anionic amino acids and cell proliferation.Biochim. Biophys. Acta 1151, 153–60.PubMedGoogle Scholar
  42. Butler, R. N., Lawson, M. J., Goland, G. J., Jarrett, I. G. &Roberts-Thomson, I. C. (1988) Proliferative activity in the proximal and distal colon of the rat after fasting and refeeding.Immunol. Cell Biol. 66, 193–8.PubMedGoogle Scholar
  43. Calza, R. E., Eckhardt, L. A., Delgiudice, T. &Schildkraut, C. L. (1984) Changes in gene position are accompanied by a change in time of replication.Cell 36, 689–96.PubMedCrossRefGoogle Scholar
  44. Camrgo, M. &Cervenka, J. (1982) Patterns of DNA replication of human chromasomes. II. Replication map and replication model.Am. J. Hum. Genet. 34, 757–80.Google Scholar
  45. Campisi, J., Gray, H. E., Pardee, A. B., Dean, M. &Sonenshein, G. E. (1984) Cell-cycle control ofc-myc but notc-ras expression is lost following chemical transformation.Cell 36, 241–7.PubMedCrossRefGoogle Scholar
  46. Carbajo, S., Gonzalez Del Pozo F. &Carbajo-Perez, E. (1992a) Quantification of the cellular proliferation on freshly dispersed cells from rat anterior pituitaries afterin vivo andin vitro labelling with bromodeoxyuridine.Histochem. J. 24, 137–43.PubMedCrossRefGoogle Scholar
  47. Carbajo, S., Hernandez, J. L. &Carbajo-Perez, E. (1992b) Proliferative activity of cells of the intermediate lobe of the rat pituitary during the postnatal period.Tissue Cell 24, 829–34.PubMedCrossRefGoogle Scholar
  48. Carbajo-Perez, E. &Watanabe, Y. G. (1990) Cellular proliferation in the anterior pituitary of the rat during the postnatal period.Cell Tissue Res. 261, 333–8.PubMedGoogle Scholar
  49. Carbajo-Perez, E., Motegi, M. &Watanabe, Y. G. (1989) Cell proliferation in the anterior pituitary of mice during growth.Biomed. Res. 10, 275–81.Google Scholar
  50. Carty, M. P., Hauser, J., Levine, A. S. &Dixon, K. (1993) Replication and mutagenesis of UV-damaged DNA templates in human and monkey cell extracts.Mol. Cell. Biol. 13, 533–542.PubMedGoogle Scholar
  51. Casas, F. C. &Jefferies, A. R. (1992a) Estimation of S phase duration in goat epidermis by an in vivo intradermal double labelling technique using bromodeoxyuridine and tritiated thymidine.Res. Vet. Sci. 52, 5–9.PubMedGoogle Scholar
  52. Casas, F. C. &Jefferies, A. R. (1992b) Comparison of intradermal injections of bromodeoxyuridine and tritiated thymidine in the in vivo measurement of epidermal cell turnover time in goats.Res. Vet. Sci. 52, 10–14.PubMedGoogle Scholar
  53. Casasco, A., Calligaro, A., Casasco, E., Marchetti, C., Poggi, P. &Casasco, M. (1988) An immunocytochemical method for studying embryo cytokinetics.Basic Appl. Histochem. 32, 293–6.PubMedGoogle Scholar
  54. Casasco, A., Calligaro, A., Marchetti, C., Poggi, P., Brugnatelli, S., Danova, M. &Fiocca, R. (1989a) Immunocytochemical detection of proliferating cells in the rat tooth germ by monoclonal antibodies against 5-bromo-2′-deoxyuridine.Arch. Oral Biol. 34, 65–9.PubMedGoogle Scholar
  55. Casasco, A., Calligaro, A., Marchetti, C., Poggi, P., Casasco, M. &Menghini, P. (1989b) [Immunocytochemical study of the development of the oral cavity in the rat]Bull. Group Int. Rech. Sci. Stomatol. Odontol. 32, 13–17.PubMedGoogle Scholar
  56. Casasco, A., Calligaro, A. &Casasco, M. (1992) Proliferative and functional stages of rat ameloblast differentiation as revealed by combined immunocytochemistry against enamel matrix proteins and bromodeoxyuridine.Cell Tissue Res. 270, 415–23.PubMedCrossRefGoogle Scholar
  57. Casasco, A., Giordano, M. Danova, M., Casasco, M., Icaro Cornaglia A. &Calligaro, A. (1993) PC10 monoclonal antibody to proliferating cell nuclear antigen as probe for cycling cell detection in developing tissues. A combined immunocytochemical and flow cytometric study.Histochemistry 99, 191–9.PubMedCrossRefGoogle Scholar
  58. Cave, M. D. (1968) Chromosome replication and synthesis of non-histone proteins in giant polytene chromosomes.Chromosoma 25, 392–401.PubMedGoogle Scholar
  59. Cawood, A. H. &Savage, J. R. (1985) Uniterrupted DNA synthesis during S-phase in untrasformed diploid hamster fibroblasts.Chromasoma 91, 164–6.Google Scholar
  60. Chamuleau, R. A., Aronsn, D. C., Frederiks, W. M., Bosman, D. K., Smit, J. J., Maas, M. A. &Jansen, P. L. (1991) Liver regeneration after partial hepatectomy in rats with defective bilirubin conjugation or biliary excretion.Dig. Dis. Sci. 36, 510–12.PubMedCrossRefGoogle Scholar
  61. Chan, E. Y. &Maclennan, I. C. (1993) Only a small proportion of splenic B cells in adults are short-lived virgin cells.Eur. J. Immunol. 23, 357–63.PubMedGoogle Scholar
  62. Chan, S. H., Cosgrove, D., Waltzinger, C., Benoist, C. &Maths, D. (1993) Another view of the selective model of thymocyte selection.Cell 73, 225–36.PubMedCrossRefGoogle Scholar
  63. Chapkin, R. S., Gao, J., Lee, Y. &Lupton, J. R. (1993) Dietary fibers and fats alter rat colon protein kinase C activity: correlation to cell probliferation.J. Nutr. 123, 649–55.PubMedGoogle Scholar
  64. Chen, Y. &Rabinovitch, P. S. (1989) Platelet-derived growth factor, epidermal growth factor, and insulin-like growth factor I regulate specific cell-cycle parameters of human diploid fibroblasts in serum-free culture.J. Cell Physiol. 140, 59–67.PubMedCrossRefGoogle Scholar
  65. Chono, Y. (1993) [Overgrowth and DNA synthesis of neuroepithelium in embryonic stages of induced Long-Evans rat meyeloschisis].Hokkaido Igaku Zasshi 68, 8–17.PubMedGoogle Scholar
  66. Christov, K., Chew, K. L., Ljung, B. M., Waldman, F. M. Duarte, L. A., Goodson, W. H., Smith, H. S. &Mayall, B. H. (1991) Proliferation of normal breast epithelial cells as shown by in vivo labelling with bromodeoxyuridine.Am. J. Pathol. 138, 1371–7.PubMedGoogle Scholar
  67. Christov, K., Swanson, S. M., Guzman, R. C., Thordarson, G., Jin, E., Talamantes, F. &Nandi, S. (1993) Kinetics of mammary epithelial cell proliferation in pituitary isografted BALB/c mice.Carcinogenesis 14, 2019–25.PubMedGoogle Scholar
  68. Ciacci, C., Lind, S. E. &Podolsky, D. K. (1993) Transforming growth factor beta regulation of migration in wounded rat intestinal epithelial monolayers.Gastroenterology 105, 93–101.PubMedGoogle Scholar
  69. Clausen, O. P., Berg, K. A., Kirkhus, B., De Angelis, P. &Huitfeldt, H. (1992) BrdUrd incorporation studies for evaluation of spermatogenesis in the blue fox.Cytometry 13, 374–80.PubMedCrossRefGoogle Scholar
  70. Clermont, Y. (1972) Kinetics of spermatogenesis in mammals. Seminiferous epithelium cycle and spermatogonial renewal.Physiol. Rev. 52, 198–232.PubMedGoogle Scholar
  71. Closset, J., Dombrowicz, D., Vandenbroeck, M. &Hennen, G. (1991) Effects of bovine, human and rat growth hormones on immature hypophysectomized rat testis.Growth Regul. 1, 29–37.PubMedGoogle Scholar
  72. Clowry, G. J. &Vrbova, G. (1992) Observations on the development of transplanted embryonic ventral horn neurones grafted into adult rat spinal cord and connected to skele muscle implants via a peripheral nerve.Exp. Brain Res. 91, 249–58.PubMedCrossRefGoogle Scholar
  73. Cohn, S. M. &Lieberman, M. W. (1984) The use of antibodies to 5-bromo-2′-deoxyuridine for the isolation of DNA sequences containing excision-repair sites.J. Biol. Chem. 259, 12456–62.PubMedGoogle Scholar
  74. Cormack, D. H. (1984).Introduction to Histology. Philadelphia: J. B. Lippincott Company.Google Scholar
  75. Corotto, F. S., Henegar, J. A. &Maruniak, J. A. (1993) Neurogenesis persists in the subependymal layer of the adult mouse brain.Neurosci. Lett. 149, 111–14.PubMedCrossRefGoogle Scholar
  76. Couldwell, W. T., Fraser G., De Vellis, G., Antel, J. P., Villemure, J. G. &Yong, V. W. (1992) Malignant glioma-derived soluble factors regulate proliferation of normal adult human astrocytes.J. Neuropathol Exp. Neurol. 51, 506–13.PubMedGoogle Scholar
  77. Couturier, J. &Dutrillaux, B. (1981) Conservation of replication chronology of homologous chromosome bands between four species of the genus Cebus and man.Cytogenet. Cell Genet. 29, 233–40.PubMedGoogle Scholar
  78. Couturier, J., Dutrillaux, B., Garber P., Raoul, O., Croquette, M., Fourlinnie, J. C. &Maillard, E. (1979). Evidence for correlation between late replication and autosomal gene inactivation in a familial translocation.Hum. Genet. 49, 319–26.PubMedCrossRefGoogle Scholar
  79. Crathorn, A. R. &Roberts, J. J. (1966) Mechanisms of the cytotoxic action of alkylating agents in mammalian cells and evidence for the removal of alkylated groups from deoxyribonucleic acid.Nature 211, 150–2.PubMedGoogle Scholar
  80. Crippen, T. L. &Jones, I. M. (1989) Cell proliferation in the bone marrow, thymus and spleen of mice studied by continuous, in vivo bromodeoxyuridine labelling and flow cytometric analysis.Cell Tissue Kinet. 22, 203–12.PubMedGoogle Scholar
  81. Czernielewski, J. M. &Demarchez, M. (1990) Further evidence for the self-reproducing capacity of Langerhans cells in human skin.J. Invest. Dematol. 88, 17–20.Google Scholar
  82. Danova, M., Lucotti, C., Rosti, V., Bergamaschi, G., Riccardi, A. &Cassola, M. (1993) In vivo growth characteristics of human CD34+ bone marrow cells after chemotherapy plus GM- or G-CSF.Blood 82 (suppl.) 497.Google Scholar
  83. Darmon, E., Pincu-Hornstein, A. &Rozen, P. (1990) A rapid and simplein vitro method for evaluating human colorectal epithelial proliferation.Arch. Pathol. Lab. Med. 114, 855–7.PubMedGoogle Scholar
  84. Darrow, J. M. &Clever, U. (1970) Chromosome activity and cell function in polytene cells. III. Growth and replication.Dev. Biol. 21, 331–48.PubMedCrossRefGoogle Scholar
  85. Davidson, P. M., Campbell, I. L., Oxbrow, L., Hutson, J. M. &Harrison, L. C. (1989) Pancreatic beta cell proliferation in rabbits demonstrated by bromodeoxyuridine labelling.Pancreas 4, 594–600.PubMedGoogle Scholar
  86. Dean, P. N., Dolbeare, F., Gratzner, H., Rice, G. C. &Gray, J. W. (1984) Cell-cycle analysis using a monoclonal antibody to BrdUrd.Cell Tissue Kinet 17, 427–36.PubMedGoogle Scholar
  87. Deenen, G. J. &Kroese, F. G. (1993) Kinetics of B cell subpopulations in peripheral lymphoid tissues: evidence for the presence of phenotypically distinct short-lived and longlived B cell subsets.Int. Immunol. 5, 735–41.PubMedGoogle Scholar
  88. De Fraissinette A., Staquet, M. J., Dezutter-Dambuyant, C., Schmitt, D. &Thivolet, J. (1988) Langerhans cells in S-phase in normal skin detected by simultaneous analysis of cell surface antigen and BrdU incorporation.J. Invest. Dermatol. 91, 603–5.PubMedGoogle Scholar
  89. De Greef, G. E., Van Staalduinen, G. J., Van Doorninck, H., Van Tol, M. J. &Hijmans, W. (1992) Age-related changes of the antigen-specific antibody formation in vitro and PHA-induced T-cell proliferation in individuals who met the health criteria of the Senieur protocol.Mech. Ageing Dev. 66, 1–14.PubMedGoogle Scholar
  90. Del Rio, J. A. &Soriano, E. (1989) Immunocytochemical detection of 5′-bromodeoxyuridine incorporation in the central nervous system of the mouse.Brain Res. Dev. Brain Res. 49, 311–17.PubMedGoogle Scholar
  91. Desbiens, X., Turque, N. &Vandebunder, B. (1992) Hydrocortisone perturbs the cell proliferation pattern during feather morphogenesis: evidence for disturbance of cephalocaudal orientation.Int. J. Dev. Biol. 36, 373–80.PubMedGoogle Scholar
  92. de Vroede, M. A., In't Veld, P. A. &Pipeleers, D. G. (1990) Deoxyribonucleic acid synthesis in cultured adult rat pancreatic B cells.Endocrinology 127, 1510–16.PubMedGoogle Scholar
  93. Di Lorenzo, T. P. &Steinberg, B. M. (1990) Laryngeal keratinocytes show variable inhibition of replication by TGF-beta.J. Cell Sci. 96, 115–19.Google Scholar
  94. Dirks, R. W., Van de Rijke, F. M., Fujishita, S., Van Der Ploeg, M. &Raap, A. K. (1993) Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes.J. Cell Sci. 104, 1187–97.PubMedGoogle Scholar
  95. Doggett, N. A., Cordeiro-Stone, M., Chae, C. B. &Kaufman, D. G. (1988) Timing of proto-oncogene replication: a possible determination of early S phase sensitivity of C3H 10T1/2 cells to transformation by chemical carcinogens.Mol. Carcinogen.1, 41–9.Google Scholar
  96. Dolbeare, F. (1995a) Bromodeoxyuridine: a diagnostic tool in biology and medicine. Part I: Historical perspectives, histochemical methods and cell kinetics.Histochem. J 27, 339–69.PubMedGoogle Scholar
  97. Dolbeare, F. (1995b) Bromodeoxyuridine: a diagnostic tool in biology and medicine. Part II: Oncology, chemotherapy and carcinogenesis.Histochem. J 27, 923–64.PubMedGoogle Scholar
  98. Dolbeare, F., Gratzner, H., Pallavicini, M. G. &Gray, J. W. (1983) Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.Proc. Natl. Acad. Sci. USA 80, 5573–7.PubMedGoogle Scholar
  99. Dolbeare, F., Selden, J. R. &Lacombe, F. (1993) Non kinetic applications of the bromodeoxyuridine immunochemical technique inDNA Cytometric Analysis (edited bySampedro, A. &Orfao, A.) pp. 195–218. Oviedo: Oviedo University Press.Google Scholar
  100. Dübel, S. &Schaller, H. C. (1990) Terminal differentiation of ectodermal epithelial stem cells ofHydra can occur in G2 without requiring mitosis or S phase.J. Cell Biol. 110, 939–45.PubMedGoogle Scholar
  101. Duschl, A., Jahn, U., Bertling, C. &Sebald, W. (1992) A comparison of assays for the response of primary human T-cells upon stimulation with interleukin-2, interleukin-4 and interleukin-7.Eur. Cytokine Network 3, 97–102.Google Scholar
  102. Dutrillaux, B. (1979) Very large analogy of chromosome banding betweenCebus capucinus (Platyrrhini) and man.Cytogenet. Cell Genet. 24, 84–94.PubMedGoogle Scholar
  103. Dutrillaux, B. &Viegas-Pequignot, E. (1981) High resolution R- and G-banding on the same preparation.Hum. Genet. 57, 93–5.PubMedCrossRefGoogle Scholar
  104. Dutrillaux, B., Couturier, J., Richer, C. L. &Viegas-Pequignot, E. (1976) Sequence of replication in 277 R- and Q-bands of human chromosomes using a BrdU treatment.Chromosoma 58, 51–61.PubMedCrossRefGoogle Scholar
  105. Edenberg, H. &Huberman, J. A. (1975) Eucaryotic chromosome replication.Ann. Rev. Genet. 9, 245–84.PubMedGoogle Scholar
  106. Edwards, C. A., Wilson, R. G., Hanlon, L. &Eastwood, M. A. (1992) Effect of the dietary fibre content of lifelong diet on colonic cellular proliferation in the rat.Gut 33, 1076–9.PubMedGoogle Scholar
  107. Egawa, K., Yoshimura, K., Kageshita, T. &Ono, T. (1988) [Cell kinetics in human anagen hair and hair follicles studied with bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody].Nippon Hifuka Gakkai Zasshi 98, 61–4.PubMedGoogle Scholar
  108. Elkind, M. M. &Kamper, C. (1970) Two forms of repair of DNA in mammalian cells following irradiation.Biophys. J. 10, 237–42.PubMedGoogle Scholar
  109. Erickson-Miller, C. L., Brennan, J. K. &Abboud, C. N. (1990) Examination of survival, proliferation and cell surface antigen expression of human monocytes exposed to macrophage colony-stimulating factor (M-CSF).Int. J. Cell Cloning 8, 346–56.PubMedGoogle Scholar
  110. Ettensohn, C. A. &Ruffins, S. W. (1993) Mesodermal cell interactions in the sea urchin embryo: properties of skeletogenic secondary mesenchyme cells.Development 117, 1275–85.PubMedGoogle Scholar
  111. Ezine, S., Lucas, B., Vicari, A., Dautigny, N., Vasseur, F. &Penit, C. (1993) A novel CD45RA+CD4+ transient thymic subpopulation in MRL-lpr/lpr mice: its relation to nonproliferating CD4-CD8-CD45RA+ tumour cells.Int. Immunol. 5, 89–96.PubMedGoogle Scholar
  112. Farmer, K. C. &Phillips, R. K. (1993) Colectomy with ileorectal anastomosis lowers rectal mucosal cell proliferation in familial adenomatous polyposis.Dis. Colon Rectum 36, 167–71.PubMedCrossRefGoogle Scholar
  113. Farnum, C. E. &Wilsman, N. J. (1993) Determination of proliferative characteristics of growth plate chondrocytes by labelling with bromodeoxyuridine.Calcif. Tissue Int. 52, 110–19.PubMedCrossRefGoogle Scholar
  114. Farquharson, C., Hesketh, J. E. &Loveridge, N. (1992a) The proto-oncogene c-myc is involved in cell differentiation as well as cell proliferation: studies on growth plate chondrocytes in situ.J. Cell. Physiol. 152, 135–44.PubMedCrossRefGoogle Scholar
  115. Farquharson, C., Whitehead, C., Rennie, S., Thorp, B. &Loveridge, N. (1992b) Cell proliferation and enzyme activities associated with the development of avian tibial dyschondroplasia: an in situ biochemical study.Bone 13, 59–67.PubMedGoogle Scholar
  116. Farquharson, C., Whitehead, C. C., Rennie, J. S. &Loveridge, N. (1993) In vivo effect of 1,25-dihydroxycholecalciferol on the proliferation and differentiation of avian chondrocytes.J. Bone Miner. Res. 8, 1081–8.PubMedGoogle Scholar
  117. Fedtsova, N. G. (1991) [The possibility of lens formation in explants of the retina and pigment epithelium of the eye in chick embryos]Ontogenez 22, 237–44.PubMedGoogle Scholar
  118. Forster, I. &Rajewsky, K. (1990) The bulk of the peripheral B-cell pool in mice is stable and not rapidly renewed from the bone marrow.Proc. Natl. Acad. Sci. USA 87, 4781–4.PubMedGoogle Scholar
  119. Forster, I., Vieira, P. &Rajewsky, K. (1989) Flow cytometric analysis of cell proliferation dynamics in the B cell compartment of the mouse.Int. Immunol. 1, 321–31.PubMedGoogle Scholar
  120. Fox, M. H., Arndt-Jovin, D. J., Jovin, T. M., Baumann, P. H. &Robert-Nicoud, M. (1991) Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts.J. Cell Sci. 99, 247–53.PubMedGoogle Scholar
  121. Frank, J. L., Lawrence, W. J. R., Banks, W. L. Jr., McKinnon, J. G., Chan, W. M. &Collins, J. M. (1992) Modulation of cell cycle kinetics in human cancer with total parenteral nutrition.Cancer 69, 1858–64.PubMedGoogle Scholar
  122. Frederiks, W. M., Marx, F., Chamuleau, R. A., Van Noorden, C. J. &James, J. (1990) Immunocytochemical determination of ploidy class-dependent bromodeoxyuridine incorporation in rat liver parenchymal cells after partial hepatectomy.Histochemistry 93, 627–30PubMedCrossRefGoogle Scholar
  123. Fritz, F. J., Pabst, R. &Binns, R. M. (1990) Lymphocyte subsets and their proliferation in a model for a delayed-type hypersensitivity reaction in the skin.Immunology 71, 508–16.PubMedGoogle Scholar
  124. Frommer, M., Paul, C. &Vincent, P. C. (1988) localization of satellite DNA sequences on human metaphase chromosomes using bromodeoxyuridine-labelled probes.Chromosoma 97, 11–18.PubMedCrossRefGoogle Scholar
  125. Fujieda, M., Murata, Y., Hayashi, H., Kambe, F., Matsui, N. &Seo, H. (1993) Effect of thyroid hormone on epidermal growth factor gene expression in mouse submandibular gland.Endocrinology 132, 121–5.PubMedCrossRefGoogle Scholar
  126. Fujita, T., Yoshimine, T., Hayakawa, T., Ushio, Y., Takemoto, O., Maruno, M., Kano, M. &Mogami, H. (1988) [Postnatal cell proliferation in the rat cerebrum: immunohistochemical study with bromodeoxyuridine (BrdU)].No To Shinkei 40, 651–5.PubMedGoogle Scholar
  127. Fuller, K. A., Kanagawa, O. &Nahm, M. H. (1993) T cells within germinal centers are specific for the immunizing antigen.J. Immunol. 151, 4505–12.PubMedGoogle Scholar
  128. Furst, A., Brown, E. H., Braunstein, J. D., &Schildkraut, C. L. (1981) Globin sequences are located in a region of early-replicating DNA in murine erythroleukemia cells.Proc. Natl. Acad. Sci. USA83, 2924–8.Google Scholar
  129. Gard, A. L. &Pfeiffer, S. E. (1990) Two proliferative stages of the oligodendrocyte lineage (A2B5+O4- and O4+GalC-) under different mitogenic control.Neuron 5, 615–25.PubMedCrossRefGoogle Scholar
  130. Gaze, R. M., Wilson, M. A. &Taylor, J. S. (1993) Optic synapses are found in diencephalic neuropils before development of the tectum inXenopus.Anat. Embryol. (Berl.) 187, 27–35.CrossRefGoogle Scholar
  131. Geard, C. R. (1973) Chromatid distribution at mitosis in culturedWallabia bicolor cells.Chromosoma 44, 301–8.PubMedCrossRefGoogle Scholar
  132. Gerlyng, P., Stokke, T., Huitfeldt, H. S., Stenersen, T., Danielsen, H. E., Grotmol, T. &Seglen, P. O. (1992) Analytical methods for the study of liver cell proliferation.Cytometry 13, 404–15.PubMedCrossRefGoogle Scholar
  133. Gonzalez-Fernandez, A., Aller, P., Sans, J. &De la Torre, C. (1992) Early and late replicating DNA involved in the G1 to S transition inAllium cepa L meristematic cells.Biol. Cell 74, 243–7.PubMedGoogle Scholar
  134. Goodrich, D. W., Wang, N. P., Qian, Y. W., Lee, E. Y. &Lee, W. H. (1991) The retinoblastoma gene product regulates progression through the G1 phase of the cell cycle.Cell 67, 293–302.PubMedCrossRefGoogle Scholar
  135. Götz, M. &Bolz, J. (1992) Formation and preservation of cortical layers in slice cultures.J. Neurobiol. 23, 783–802.PubMedCrossRefGoogle Scholar
  136. Gray, D. (1988) Population kinetics of rat peripheral B cells.J. Exp. Med. 167, 805–16.PubMedGoogle Scholar
  137. Green, P., Hartenstein, A. Y. &Hartenstein, V. (1993) The embryonic development of theDrosophila visual system.Cell Tissue Res. 273, 583–98.PubMedCrossRefGoogle Scholar
  138. Grenier, Y., Ruijs, T. C., Olivier, A., Robitaille, Y. &Antel, J. P. (1989) DNA synthesis by young adult human-derived astrocytes in vitro.Brain Res. 480, 87–91.PubMedCrossRefGoogle Scholar
  139. Gressner, A. M., Lotfi, S., Gressner, G. &Lahme, B. (1992) Identification and partial characterization of a hepatocyte-derived factor promoting proliferation of cultured fat-storing cells (parasinusoidal lipocytes).Hepatology 16, 1250–66.PubMedCrossRefGoogle Scholar
  140. Guthrie, S., Butcher, M. &Lumsden, A. (1991) Patterns of cell division and interkinetic nuclear migration in the chick embryo hindbrain.J. Neurobiol. 22, 742–54.PubMedCrossRefGoogle Scholar
  141. Haase, S. B. &Calos, M. P. (1991) Replication control of autonomously replicating human sequences.Nucleic Acids Res. 19, 5053–8.PubMedGoogle Scholar
  142. Hadrava, V., Tremblay, J., Sekaly, R. P. &Hamet, P. (1992) Accelerated entry of aortic smooth muscle cells from spontaneously hypertensive rats into the S phase of the cell cycle.Biochem. Cell Biol. 70, 599–604.PubMedGoogle Scholar
  143. Hägele, K. (1970) DNS-Replikationsmuster der Speicheldrüsen-Chromosomen von Chironomiden.Chromosoma 31, 91–138.PubMedCrossRefGoogle Scholar
  144. Hagglund, B. &Sandberg, G. (1993) Effect ofl-alanine and some other amino acids on thymocyte proliferation in vivo.Immunobiology 188, 62–9.PubMedGoogle Scholar
  145. Hamada, H. (1993) [Effects of medium chain triglyceride administration on liver regeneration after partial hepatectomy in rats]Hokkaido Igaku Zasshi 68, 96–109.PubMedGoogle Scholar
  146. Hammar, S. P., Luu, J. Y., Bockus, D. E., Remington, F. L., Luu, J. W., Friedman, S. &Bean M. A. (1992) Induction of tubuloreticular structures in cultured human endothelial cells by recombinant interferon alfa and beta.Ultrastruct. Pathol. 16, 211–18.PubMedGoogle Scholar
  147. Han, H., Iwanaga, T., Uchiyama, Y. &Fujita, T. (1993) Aggregation of macrophages in the tips of intestinal villi in guinea pigs: their possible role in the phagocytosis of effete epithelial cells.Cell Tissue Res. 271, 407–16.PubMedGoogle Scholar
  148. Hanazono, M., Yoshiki, A., Ota, K., Kitoh, J. &Kusakabe, M. (1990a) DNA replication in uterine cells of adult and prepubertal mice under normal and hormonally stimulated conditions detected by bromodeoxyuridine labelling method.Endocrinol. Jpn. 37, 183–91.PubMedGoogle Scholar
  149. Hanazono, M., Yoshiki, A., Ota, K., Kitoh, J. &Kusakabe, M. (1990b) Immunohistochemical detection of DNA replication in mouse uterine cells by bromodeoxyuridine labeling of wax- and resin-embedded tissue sections.Stain Technol. 65, 139–49.PubMedGoogle Scholar
  150. Hanke, H., Strohschneider, T., Oberhoff, M., Betz, E. &Karsch, K. R. (1990) Time course of smooth muscle cell proliferation in the intima and media of arteries following experimental angioplasty.Circ. Res. 67, 651–9.PubMedGoogle Scholar
  151. Hanke, H., Haase, K. K., Hanke, S., Oberhoff, M., Hassenstein, S., Betz, E. &Karsch, K. R. (1991) Morphological changes and smooth muscle cell proliferation after experimental excimer laser treatment.Circulation 83, 1380–9.PubMedGoogle Scholar
  152. Harris, A. J., Duxson, M. J., Fitzsimons, R. B. &Rieger, F. (1989) Myonuclear birthdates distinguish the origins of primary and secondary myotubes in embryonic mammalian skeletal muscles.Development 107, 771–84.PubMedGoogle Scholar
  153. Hartmann, C. &Rode, A. (1982) [Changes, during a synchronous cell cycle, of the level of incorporation of 5-bromodeoxyuridine into DNA of chick embryo fibroblasts].C. R. Seances Acad. Sci. III 295, 69–72.PubMedGoogle Scholar
  154. Hashiguchi, J., Ito, M. &Sekine, I. (1993) The effect of the autonomic nervous system on cell proliferation of the gastric mucosa in stress ulcer formation.J. Autonom Nerv. Syst. 43, 179–87.CrossRefGoogle Scholar
  155. Hashino, E. &Salvi, R. J. (1993) Changing spatial patterns of DNA replication in the noise-damaged chick cochlea.J. Cell Sci. 105, 23–31.PubMedGoogle Scholar
  156. Hashiro, M., Matsumoto, K., Okumura, H., Hashimoto, K. &Yoshikawa, K. (1991) Growth inhibition of human keratinocytes by antisense c-myc oligomer is not coupled to induction of differentiation.Biochem. Biophys. Res. Commun. 174, 287–92.PubMedCrossRefGoogle Scholar
  157. Hassenstein, S., Hanke, H., Kamenz, J., Oberhoff, M., Hanke, S., Riessen, R., Haase, K. K., Betz, E. &Karsch, K. R. (1992) Vascular injury and time course of smooth muscle cell proliferation after experimental holmium laser angioplasty.Circulation 86, 1575–83.PubMedGoogle Scholar
  158. Hines, J. E., Johnson, S. J. &Burt, A. D. (1993) In vivo responses of macrophages and perisinusoidal cells to cholestatic liver injury.Am. J. Pathol. 142, 511–18.PubMedGoogle Scholar
  159. Hirai, R., Oguchi, Y., Sugita, N., Matsunaga, K. &Nomoto, K. (1993) Enhancement of T-cell proliferation by PSK.Int. J. Immunopharmacol. 15, 745–50.PubMedCrossRefGoogle Scholar
  160. Hirning, U., Schmid, P., Schulz, W. A., Rettenberger, G. &Hameister, H. (1991) A comparative analysis of N-myc and c-myc expression and cellular proliferation in mouse organogenesis.Mech. Dev. 33, 119–25.PubMedCrossRefGoogle Scholar
  161. Hitchcock, P. F., Lindsey Myhr K. J., Easter, S. S. Jr, Mangionesmith, R. &Jones, D. D. (1992) Local regeneration in the retina of the goldfish.J. Neurobiol. 23, 187–203.PubMedCrossRefGoogle Scholar
  162. Hogen Esch, H., Gijbels, M. J. J., Offerman, E., Van Hooft, J., Van Bekkum, D. W. &Zurcher, C. (1993) A spontaneous mutation characterized by chronic proliferative dermatitis in C57BL mice.Am. J. Pathol. 143, 972–82.Google Scholar
  163. Holmquist, G. P. (1987) Role of replication in the control of tissue-specific gene expression.Am. J. Hum. Genet. 40, 151–73.PubMedGoogle Scholar
  164. Hong, S. T., Kho, W. G., Kim, W. H., Chai, J. Y. &Lee, S. H. (1993) Turnover of biliary epithelial cells inClonorchis sinensis infected rats.Kisaengchunghak Chapchi 31, 83–9.Google Scholar
  165. Huesmann, M., Scott, B., Kisielow, P. &Von Boehmer, H. (1991) Kinetics and efficacy of positive selection in the thymus of normal and T cell receptor transgenic mice.Cell 66, 533–40.PubMedCrossRefGoogle Scholar
  166. Humbert, C. &Usson, Y. (1992) Eukaryotic DNA replication is a topographically ordered process.Cytometry 13, 603–14.PubMedCrossRefGoogle Scholar
  167. Humbert, C., Santisteban, M. S., Usson, Y. &Robert-Nicoud, M. (1992) Intranuclear co-location of newly replicated DNA and PCNA by simultaneous immunofluorescent labelling and confocal microscopy in MCF-7 cells.J. Cell Sci. 103, 97–103.PubMedGoogle Scholar
  168. Hume, W. J. (1989) DNA-synthesizing cells in oral epithelium have a range of cell cycle durations: evidence from double-labelling studies using tritiated thymidine and bromodeoxyuridine.Cell Tissue Kinet. 22, 377–82.PubMedGoogle Scholar
  169. Hume, W. J. &Thompson, J. (1990) Double labelling of cells with tritiated thymidine and bromodeoxyuridine reveals a circadian rhythm-dependent variation in duration of DNA synthesis and S phase flux rates in rodent oral epithelium.Cell Tissue Kinet. 23, 313–23.PubMedGoogle Scholar
  170. Huong, P. L., Kolk, A. H., Eggelte, T. A., Verstijnen, C. P., Gilis, H. &Hendriks, J. T. (1991) Measurement of antigen specific lymphocyte proliferation using 5-bromo-deoxyuridine incorporation. An easy and low cost alternative to radio-active thymidine incorporation.J. Immunol. Methods 140, 243–8.PubMedCrossRefGoogle Scholar
  171. Hurme, T. &Kalimo, H. (1992) Activation of myogenic precursor cells after muscle injury.Med. Sci. Sports Exercise 24, 197–205.Google Scholar
  172. Iannuzzi, L., Gustavsson, I., Di Meo G. P. &Ferrara, L. (1989) High-resolution studies on late-replicating segments (G+C bands) in mammalian chromosomes.Hereditas 110, 43–50.PubMedGoogle Scholar
  173. Irani, A. M., Nilsson, G., Miettinen, U., Craig, S. S., Ashman, L. K., Ishizaka, T., Zsebo, K. M. &Schwartz, L. B. (1992) Recombinant human stem cell factor stimulates differentiation of mast cells from dispersed human fetal liver cells.Blood 80, 3009–21.PubMedGoogle Scholar
  174. Ito, K. &Hotta, Y. (1992) Proliferation pattern of postembryonic neuroblasts in the brain ofDrosophila melanogaster.Dev. Biol. 149, 134–48.PubMedCrossRefGoogle Scholar
  175. Ito, K. &McGhee, J. D. (1987) Parental DNA strands segregate randomly during embryonic development ofCaenorhabditis elegans.Cell 49, 329–36.PubMedCrossRefGoogle Scholar
  176. Ito, K., Mcghee, J. D. &Schultz, G. A. (1988) Paternal DNA strands segregate to both trophectoderm and inner cell mass of the developing mouse embryo.Genes Devel. 2, 929–36.PubMedGoogle Scholar
  177. Ito, Y. (1993) [DNA synthesis of the mucosa of the gallbladder estimated by bromodeoxyuridine (BrdU)-anti BrdUin vitro method in patients with various gallbladder disorders]Nippon Shokakibyo Gakkai Zasshi 90, 665–72.PubMedGoogle Scholar
  178. Jablonka-Shariff, A., Grazul-Bilska, A. T., Redmer, D. A. &Reynolds, L. P. (1993) Growth and cellular proliferation of ovine corpora lutea throughout the estrous cycle.Endocrinology 133, 1871–9.PubMedCrossRefGoogle Scholar
  179. Jezequel, A. M., Paolucci, F., Benedetti, A., Mancini, R. &Orlandi, F. (1991) Enumeration of S-phase cells in normal rat liver by immunohistochemistry using bromodeoxyuridine-antibromodeoxyuridine system.Dig. Dis. Sci. 36, 482–4.PubMedCrossRefGoogle Scholar
  180. Jirikowski, G. F., Ramalho-Ortigao, J. F., Lindl, T. &Seliger, H. (1989) Immunocytochemistry of 5-bromo-2′-deoxyuridine labelled oligonucleotide probes. A novel technique for in situ hybridization.Histochemistry 91, 51–3.PubMedCrossRefGoogle Scholar
  181. Jirikowski, G. F., Ramalho-Ortigao, J. F., Kesse, K. W. &Bloom, F. E. (1990) In situ hybridization of semithin Epon sections with BrdU labelled oligonucleotide probes.Histochemistry 94, 187–90.PubMedGoogle Scholar
  182. Johnson, D. G., Schwarz, J. K., Cress, W. D. &Nevins, J. R. (1993) Expression of transcription factor E2F1 induces quiescent cells to enter S phase.Nature 365, 349–52.PubMedCrossRefGoogle Scholar
  183. Johnson, S. J., Hines, J. F. &Burt, A. D. (1992) Macrophage and perisinusoidal cell kinetics in acute liver injury.J. Pathol. 166, 351–8.PubMedCrossRefGoogle Scholar
  184. Jones, H. B., Clarke, N. A. &Barrass, N. C. (1993) Phenobarbital-induced hepatocellular proliferation: anti-bromodeoxyuridine and anti-proliferating cell nuclear antigen immunocytochemistry.J. Histochem. Cytochem 41, 21–7.PubMedGoogle Scholar
  185. Just, W., Autenrieth, M. &Vogel, W. (1993) Demonstration of replication patterns on histone-depleted chromosomes.Cytogenet. Cell Genet. 62, 60–3.PubMedGoogle Scholar
  186. Kalle, W. H. J., Hazekamp-Van Dokkum, A.-M., Lohman, P. H. M., Natarajan, A. T., Van Zeeland, A. A. &Mullenders, L. H. F. (1993) The use of streptavidin-coated magnetic beads and biotinylated antibodies to investigate induction and repair of DNA damage: analysis of repair patches in specific sequences of uv-irradiated human fibroblasts.Anal. Biochem. 208, 228–36.PubMedCrossRefGoogle Scholar
  187. Kamiryo, T., Orita, T., Nishizaki, T. &Aoki, H. (1990) Development of the rat meninx: experimental study using bromodeoxyuridine.Anat. Rec. 227, 207–10.PubMedCrossRefGoogle Scholar
  188. Kanai-Azuma, M., Kanal, Y., Kurohmaru, M., Sakai, S. &Hayashi, Y. (1993) Insulin-like growth factor (IGF)-I stimulates proliferation and migration of mouse ectoplacental cone cells, while IGF-II transforms them into trophoblastic giant cells in vitro.Biol. Reprod. 48, 252–61.PubMedCrossRefGoogle Scholar
  189. Karasek, M., Liberski, P., Kunert-Radek, J. &Bartkowiak, J. (1992) Influence of melatonin on the proliferation of hepatoma cells in the Syrian hamster: in vivo and in vitro study.J. Pineal Res. 13, 107–10.PubMedGoogle Scholar
  190. Karn, J., Watson, J. V., Lowe, A. D., Green, S. M. &Vedeckis, W. (1989) Regulation of cell cycle duration by c-myc levels.Oncogene 4, 773–7.PubMedGoogle Scholar
  191. Karube, T. &Watanabe, S. (1988) Analysis of the chromosommal DNA replication pattern using the bromodeoxyuridine labelling method.Cancer Res. 48, 219–22.PubMedGoogle Scholar
  192. Karube, T., Ochi-Takeuchi, H. &Watanabe, S. (1987) A new immunocytochemical method to measure cell kinetics of human lymphocytes by using monoclonal antibody against bromodeoxyuridine.Nippon Ketsueki Gakkai Zasshi 50, 862–7.PubMedGoogle Scholar
  193. Kassem, M., Blum, W., Ristelli, J., Mosekilde, L. &Eriksen, E. F. (1993) Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro.Calcif. Tissue Int. 52, 222–6.PubMedCrossRefGoogle Scholar
  194. Katelaris, A., Penny, R. &Breit, S. N. (1992) Mitotic activity in the dermis of mice during graft versus host disease: the role of fibroblast replication in dermal fibrosis.J. Invest. Dermatol. 99, 779–83.PubMedCrossRefGoogle Scholar
  195. Katoh, K., Kasai, S. &Mito, M. (1989) [Effect of activation of the reticuloendothelial system on hepatocyte regeneration in partially hepatectomized rats]Nippon Geka Gakkai Zasshi 90, 250–7.PubMedGoogle Scholar
  196. Katsuda, S., Coltrera, M. D., Ross, R. &Gown, A. M. (1993) Human atherosclerosis. IV. Immunocytochemical analysis of cell activation and proliferation in lesions of young adults.Am. J. Pathol. 142, 1787–93.PubMedGoogle Scholar
  197. Kaufman, S. J. &Foster, R. F. (1988) Replicating myoblasts express a muscle-specific phenotype.Proc. Natl. Acad. Sci. USA 85, 9606–10.PubMedGoogle Scholar
  198. Kaufman, S. J. &Robert-Nicoud, M. (1985) DNA replication and differentiation in rat myoblasts studied with monoclonal antibodies against 5-bromodeoxyuridine, actin, and alpha 2-macroglobulin.Cytometry 6, 570–7.PubMedCrossRefGoogle Scholar
  199. Kelic, S., Olsson, T. &Kristensson, K. (1993) Interferon-gamma promotes proliferation of rat skeletal muscle cellsin vitro and alters their AChr distribution.J. Neurol. Sci. 114, 62–7.PubMedCrossRefGoogle Scholar
  200. Kellett, M., Potten, C. S. &Rew, D. A. (1992) A comparison ofin vivo cell proliferation measurements in the intestine of mouse and man.Epithelial Cell Biol. 1, 147–55.PubMedGoogle Scholar
  201. Kennerknecht, I., Baur-Aubele, S. &Vogel, W. (1991) Proliferation kinetics in native chorionic villus cells.Prenat. Diagn. 11, 591–5.PubMedGoogle Scholar
  202. Kimura, Y., Kamide, M., Furukawa, M., Miwa, T., Sakumoto, M. &Umeda, R. (1990) [Evaluation of turnover of olfactory epithelium in mice by using anti-BrdU monoclonal antibody].Nippon Jibiinkoka Gakkai Kaiho 93, 165–70.PubMedGoogle Scholar
  203. Kirkhus, B. &Clausen, O. P. (1990) Cell kinetics in mouse epidermis studied by bivariate DNA/bromodeoxyuridine and DNA/keratin flow cytometry.Cytometry 11, 253–60.PubMedCrossRefGoogle Scholar
  204. Kirkhus, B. &Clausen, O. P. (1992) Circadian variations in cell cycle phase progression of mouse epidermal cells measured directly by bivariate BrdUrd/DNA flow cytometry.Epithelial Cell Biol. 1, 32–8.PubMedGoogle Scholar
  205. Kitao, Y., Okoyama, S., Moriizumi, T. &Kudo, M. (1993) Neurogenetical segregation of the vestibulospinal neurons in the rat.Brain Res. 620, 149–54.PubMedCrossRefGoogle Scholar
  206. Kitazawa, R., Kitazawa, S., Fukase, M., Fujita, T., Kobayashi, A., Chihara, K. &Maeda, S. (1992) The expression of parathyroid hormone-related protein (PTHrP) in normal parathyroid: histochemistry, and in situ hybridization.Histochemistry 98, 211–15.PubMedCrossRefGoogle Scholar
  207. Kitazawa, S., Takenaka, A., Abe, N., Maeda, S., Horio, M. &Sugiyama, T. (1989) In situ DNA-RNA hybridization using in vivo bromodeoxyuridine-labeled DNA probe.Histochemistry 92, 195–9.PubMedCrossRefGoogle Scholar
  208. Kitazawa, S., Kitazawa, R., Gotoh, A., Fujimori, T. &Maeda, S. (1993) In situ hybridization using bromodeoxyuridine labelled DNA probe and RNase H.Biotech Histochem. 68, 137–41.PubMedGoogle Scholar
  209. Kleibeuker, J. H., Welberg, J. W., Mulder, N. H., Van Der Meer, R., Cats, A., Limburg, A. J., Kreumer, W. M., Hardonk, M. J. &De Vries, E. G. (1993) Epithelial cell proliferation in the sigmoid colon of patients with adenomatous polyps increases during oral calcium supplementation.Br. J. Cancer 67, 500–3.PubMedGoogle Scholar
  210. Kleine, H. D., Wagner, U., Poliwoda, H. &Freund, M. (1992) Effects of tumor necrosis factor alpha on bone marrow aspirates of patients with acute myelogenous leukemia determined by flow-cytometric cell-cycle analysis.J. Cancer Res. Clin. Oncol. 118, 56–60.PubMedCrossRefGoogle Scholar
  211. Knapp, P. E. (1991) Studies of glial lineage and proliferationin vitro using an early marker for committed oligodendrocytes.J. Neurosci. Res. 30, 336–45.PubMedCrossRefGoogle Scholar
  212. Knapp, P. E. (1992) The cell cycle of glial cells grown in vitro: an immunocytochemical method of analysis.J. Histochem. Cytochem. 40, 1405–11.PubMedGoogle Scholar
  213. Knight, J. K. &Raymond, P. A. (1990) Time course of opsin expression in developing rod photoreceptors.Development 110, 1115–20.PubMedGoogle Scholar
  214. Kohn, K. W. &Grimek-Ewig, R. A. (1973) Alkaline elution analysis, a new approach to the study of DNA single-strand interruptions in cells.Cancer Res. 33, 1849–53.PubMedGoogle Scholar
  215. Koike, N., Saitoh, K., Todoroki, T., Kawamoto, T., Iwasaki, Y. &Nakamura, K. (1993) Cell proliferation kinetics of the bile duct epithelium of the rat.Cell Prolif. 26, 183–93.PubMedGoogle Scholar
  216. Koizumi, O., Itazawa, M., Mizumoto, H., Minobe, S., Javois, L. C., Grimmelikhuijzen, C. J. &Bode, H. R. (1992) Nerve ring of the hypostome in hydra. I. Its structure, development, and maintenance.J. Comp. Neurol. 326, 7–21.PubMedCrossRefGoogle Scholar
  217. Kojima, I., Mogami, H., Kawamura, N., Yasuda, H. &Shibata, H. (1993) Modulation of growth of vascular smooth muscle cells by activin A.Exp. Cell Res. 206, 152–6.PubMedCrossRefGoogle Scholar
  218. Kosaka, T., Kamata, T., Ooyama, S., Kanno, M., Yamaguchi, A., Yonemura, Y., Miwa, K. &Miyazaki, I. (1990) [A flow cytometric cell cycle analysis of familial polyposis coli].Gan No Rinsho 36, 2141–5.PubMedGoogle Scholar
  219. Kroeger, H., Gettmann, W. &Kalater, C. H. (1973) Zur Kontrolle der DNA-Replication in Riesenchromosomen explantierter Speicheldrüsen.Cytobiologie 7, 65–88.Google Scholar
  220. Kruse, F. E. &Tseng, S. C. G. (1993a) Growth factors modulate clonal growth and differentiation of cultured rabbit limbal and corneal epithelium.Invest. Ophthalmol. Vis. Sci. 34, 1963–76.PubMedGoogle Scholar
  221. Kruse, F. E. &Tseng, S. C. G. (1993b) A tumor promoter-resistant subpopulation of progenitor cells is larger in limbal epithelium than in corneal epithelium.Invest. Ophthalmol. Vis. Sci. 34, 2501–11.PubMedGoogle Scholar
  222. Krushel, L. A., &Van Der Kooy, D. (1993) Pattern formation in the developing mammalian forebrain: selective adhesion of early but not late postmitotic cortical and striatal neurons within forebrain reaggregate cultures.Dev. Biol. 158, 145–62.PubMedCrossRefGoogle Scholar
  223. Kubbies, M., Friedl, R., Koehler, J., Rabinovitch, P. S. &Hoehn, H. (1990) Improvement of human lymphocyte proliferation and alteration of IL-2 secretion kinetics by alpha-thioglycerol.Lymphokine Res. 9, 95–106.PubMedGoogle Scholar
  224. Kulomaa, P., Paavonen, J. &Lehtinen, M. (1992) Herpes simplex virus induces unscheduled DNA synthesis in virus-infected cervical cancer cell lines.Res. Virol. 143, 351–9.PubMedGoogle Scholar
  225. Kuni, Y., Tarao, K., Shimizu, A., Nakamura, N., Harada, M., Ito, Y., Iimori, K. &Tamai, S. (1988) [Evaluation of DNA synthesis using antibromodeoxyuridine antibody in liver cirrhosis and chronic active hepatitis]Nippon Shokakibyo Gakkai Zasshi 85, 2187–92.PubMedGoogle Scholar
  226. Kusewitt, D. F., Budge, C. L., Nolla, H. A., Edwards, B. S. &Ley, R. D. (1992) Cell cycle progression in den V-transfected murine fibroblasts exposed to ultraviolet radiation.Mutat. Res. 274, 163–76.PubMedGoogle Scholar
  227. Kuyatt, B. L., Reidy, C. A., Hui, K. Y. &Jordan, W. H. (1993) Quantitation of smooth muscle proliferation in cultured aorta. A color image analysis method for the Macintosh.Anal. Quant. Cytol. Histol. 15, 83–7.PubMedGoogle Scholar
  228. Labrecque, C., Huard, J., Dansereau, G., Albert, N. &Tremblay, J. P. (1991) In vitro bromodeoxyuridine labelling of nuclei: application to myotube hybridization.J. Histochem. Cytochem. 39, 1421–6.PubMedGoogle Scholar
  229. Lacy, E. R., Kuwayama, H., Cowart, K. S., King, J. S., Deutz, A. H. &Sistrunk, S. (1991) A rapid, accurate, immunohistochemical method to label proliferating cells in the digestive tract. A comparison with tritiated thymidine.Gastroenterology 100, 259–62.PubMedGoogle Scholar
  230. Lagra, F., Raptopoulou-Gigi, M., Orphanou-Koumerkeridou, H. &Goulis, G. (1989) In vitro effect of interferon alpha-2b on T lymphocyte transformation and leukocyte migration inhibition in patients with chronic brucellosis.Immunopharmacol. Immunotoxicol. 11, 223–32.PubMedGoogle Scholar
  231. Lane, H. A., Fernandez, A., Lamb, N. J. &Thomas, G. (1993) p70s6k function is essential for G1 progression.Nature 363, 170–2.PubMedCrossRefGoogle Scholar
  232. Lantz, O., Alard, P., Chavanel, G., Fries, D., Senik, A. &Charpentier, B. (1991) EBV cell-lines (LCL) and E-cells as stimulator cells for limiting dilution analysis (LDA) of alloreactive IL-2-secreting cells (IL-2-SC) and cytotoxic precursors (CTLp). Comparison of their stimulator capacity and ability to generate specific alloreactivity.J. Immunol. Methods 137, 121–32.PubMedCrossRefGoogle Scholar
  233. Lardon, F., Van Bockstaele, D. R., Snoeck, H. W. &Peetermans, M. E. (1993) Quantitative cell-cycle progression analysis of the first three successive cell cycles of granulocyte colony-stimulating factor and/or granulocyte-macrophage colony-stimulating factor-stimulated human CD34+ bone marrow cells in relation to their colony formation.Blood 81, 3211–16.PubMedGoogle Scholar
  234. Larison, K. D. &Bremiller, R. (1990) Early onset of phenotype and cell patterning in the embryonic zebrafish retina.Development 109, 567–76.PubMedGoogle Scholar
  235. Larramendy, M. L. &Knuutila, S. (1990) Immunophenotype and sister chromatid differentiation: a combined methodology for analyzing cell proliferation in unfractionated lymphocyte cultures.Exp. Cell Res. 188, 209–13.PubMedCrossRefGoogle Scholar
  236. Larson, J. L., Wolf, D. C. &Butterworth, B. E. (1993) Acute hepatotoxic and nephrotoxic effects of chloroform in male F-344 rats and female B6C3F1 mice.Fundam. Appl. Toxicol. 20, 302–15.PubMedGoogle Scholar
  237. Latos-Bielenska, A. &Vogel, W. (1990) Frequency and distribution of chiasmata in Syrian hamster spermatocytes studied by the BrdU antibody technique.Chromosoma 99, 267–72.PubMedCrossRefGoogle Scholar
  238. Latos-Bielenska, A. &Vogel, W. (1992) Demonstration of replication patterns in the last premeiotic S-phase of male Chinese hamsters after BrdU pulse labelling.Chromosoma 101, 279–83.PubMedGoogle Scholar
  239. Latos-Bielenska, A., Hameister, H. &Vogel, W. (1987) Detection of BrdUrd incorporation in mammalian chromosomes by a BrdUrd antibody. III. Demonstration of replication patterns in highly resolved chromosomes.Hum. Genet. 76, 293–5.PubMedCrossRefGoogle Scholar
  240. Latt, S. A. (1973) Microfluorimetric detection of deoxyribonucleic acid replication in human metaphase chromosomes.Proc. Natl. Acad. Sci. USA.70, 3395–9.PubMedGoogle Scholar
  241. Lau, Y. F. &Arrighi, F. E. (1981) Studies of mammalian chromosome replication. II. Evidence for the existence of defined chromosome replicating units.Chromasoma 83, 721–41.Google Scholar
  242. Layer, P. G. &Alber, R. (1990) Patterning of chick brain vesicles as revealed by peanut agglutinin and cholinesterases.Development 109, 613–24.PubMedGoogle Scholar
  243. Lee, D. Y., Chapkin, R. S., Lupton, J. R. (1993) Dietary fat and fiber modulate colonic cell proliferation in an interactive sitespecific manner.Nutr. Cancer 20, 107–18.PubMedGoogle Scholar
  244. Lehrnbecher, T., Merz, H., Sebald, W. &Poot, M. (1991) Interleukin 4 drives phytohemagglutinin-activated T cells through several cell cycles: no synergism between interleukin 2 and interleukin 4.Cytokine 3, 593–7.PubMedCrossRefGoogle Scholar
  245. Lehtinen, M., Kulomaa, P., Kallioniemi, O. P., Paavonen, J. &Leinikki, P. (1989) Analysis of DNA synthesis in herpes simplex virus infected cells by dual parameter flow cytometry.Arch. Virol. 107, 215–23.PubMedCrossRefGoogle Scholar
  246. Lett, J. T., Klucis, E. A. &Sun, C. (1970) On the size of the DNA in the mammalian chromosome.Biophys. J. 10, 277–82.PubMedGoogle Scholar
  247. Levin, B. (1992) Nutrition and colorectal cancer.Cancer 70, 1723–6.PubMedGoogle Scholar
  248. Ley, R. D., Applegate, L. A., Deriel, J. K. &Henderson, E. E. (1989) Excision repair characteristics of DENV-transformed Xeroderma pigmentosum cells.Mutat. Res. 217, 101–7.PubMedGoogle Scholar
  249. Likhachev, A. J., Tomatis, L. &Margison, G. P. (1983) Incorporation and persistence of 5-bromodeoxyuridine in newborn rat tissue DNA.Chem. Biol. Interact. 46, 31–8.PubMedGoogle Scholar
  250. Loizidou, M. C., Lawrance, R. J., Holt, S., Carty, N. J., Cooper, A. J., Alexander, P. &Taylor, I. (1991) Facilitation by partial hepatectomy of tumor growth within the rat liver following intraportal injection of syngeneic tumor cells.Clin. Exp. Metastasis 9, 335–49.PubMedCrossRefGoogle Scholar
  251. Lopez-Girona, A., Colomer, J., Pujol, M. J., Bachs, O. &Agell, N. (1992) Calmodulin regulates DNA polymerase alpha activity during proliferative activation of NRK cells.Biochem. Biophys. Res. Commun. 184, 1517–23.PubMedGoogle Scholar
  252. Lorenzi, M., Nordberg, J. A. &Toledo, S. (1987) High glucose prolongs cell-cycle traversal of cultured human endothelial cells.Diabetes 36, 1261–7.PubMedGoogle Scholar
  253. Love, T. C. &Tucker, D. C. (1993) Possible paracrine facilitation of ventricular growth in oculo by atrial tissue.Am. J. Physiol. 265, 1082–8.Google Scholar
  254. Lucas, B., Vasseur, F. &Penit, C. (1993) Normal sequence of phenotypic transitions in one cohort of 5-bromo-2-deoxyuridine-pulse-labeled thymocytes. Correlation with T cell receptor expression.J. Immunol. 151, 4574–82.PubMedGoogle Scholar
  255. Lukomska, B., Pienkowska, B., Andrzejewski, W. &Olszewski, W. L. (1991) Liver sinusoidal cytotoxic cells are recruited from blood and divide locally.J. Hepatol. 12, 332–5.PubMedCrossRefGoogle Scholar
  256. Lundy, M. W., Hendrix, T., Wergedal, J. E. &Baylink, D. J. (1991) Growth factor-induced proliferation of osteoblasts measured by bromodeoxyuridine immunocytochemistry.Growth Factors 4, 257–64.PubMedGoogle Scholar
  257. Lutzow-Holm, C., De Angelis, P. &Clausen, O. P. (1992) Retinoic acid provokes a regeneration-like proliferative response in murine epidermis. A bivariate DNA/bromodeoxyuridine flow cytometric study.Arch. Dermatol. Res. 284, 418–23.PubMedGoogle Scholar
  258. Ma, C., Leu, T. H. &Hamlin, J. L. (1990) Multiple origins of replication in the dihydrofolate reductase amplicons of a methotrexate-resistant chinese hamster cell line.Mol. Cell. Biol. 10, 1338–46.PubMedGoogle Scholar
  259. Ma, W., Behar, T., Maric, D., Maric, I. &Barker, J. L. (1992) Neuroepithelial cells in the rat spinal cord express glutamate decarboxylase immunoreactivity in vivo and in vitro.J. Comp. Neurol. 325, 257–70.PubMedGoogle Scholar
  260. Mackay, C. R., Marston, W. L. &Dudler, L. (1990) Naive and memory T cells show distinct pathways of lymphocyte recirculation.J. Exp. Med. 171, 801–17.PubMedCrossRefGoogle Scholar
  261. Maggio-Price, L., Grossmann, A., Shiota, F. &Engel, D. (1993) Increased proliferative capacity of CD4+ and CD8+T lymphocytes from mutant sphha/sphha mice is associated with increased IL-2 receptor expression.Cell. Immunol. 148, 346–56.PubMedCrossRefGoogle Scholar
  262. Mah, D. C. W., Dijkwel, P. A., Todd, A., Klein, V., Price, G. B. &Zannis-Hadjopoulos, M. (1993)ors12, a mammalian autonomously replicating DNA sequence, associates with the nuclear matrix in a cell cycle-dependent manner.J. Cell Sci. 105, 807–18.PubMedGoogle Scholar
  263. Mancini, R., Jezequel, A. M., Benedetti, A., Paolucci, F., Trozzi, L. &Orlandi, F. (1992) Quantitative analysis of proliferating sinusoidal cells in dimethylnitrosamine-induced cirrhosis. An immunohistochemical study.J. Hepatol. 15, 361–6.PubMedCrossRefGoogle Scholar
  264. Manders, E. M. M., Stap, J., Brakenhoff, G. J., Van Driel, R. &Aten, J. (1992) Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy.J. Cell Sci. 103, 857–62.PubMedGoogle Scholar
  265. Maniotis, A. &Schliwa, M. (1991) Microsurgical removal of centrosomes blocks cell reproduction and centriole generation in BSC-1 cells.Cell 67, 495–504.PubMedCrossRefGoogle Scholar
  266. Manson, M. M., Green, J. A., Wright, B. J. &Carthew, P. (1992) Degree of ethoxyquin-induced nephrotoxicity in rat is dependent on age and sex.Arch. Toxicol. 66, 51–6.PubMedCrossRefGoogle Scholar
  267. Mark, M. P., Bloch-Zupan, A. &Ruch, J. V. (1992) Effects of retinoids on tooth morphogenesis and cytodifferentiations, in vitro.Int. J. Dev. Biol. 36, 517–26.PubMedGoogle Scholar
  268. Marshall, M. J. &Davie, M. W. (1991) An immunocytochemical method for studying the kinetics of osteoclast nuclei on intact mouse parietal bone.Histochem. J. 23, 402–8.PubMedCrossRefGoogle Scholar
  269. Marucci, I., Baroni, G. S., Mancini, R., Benedetti, A., Jezequel, A. M. &Orlandi, F. (1993) Cell proliferation following extrahepatic biliary obstruction. Evaluation by immunohistochemical methods.J. Hepatol 17, 163–9.PubMedCrossRefGoogle Scholar
  270. Marushige, Y., Marushige, K. &Koestner, A. (1992) Growth inhibition of anaplastic glioma cells by nerve growth factor.Anticancer Res. 12, 2069–73.PubMedGoogle Scholar
  271. Mashiba, H., Matsunaga, K., Yagawa, K., Nomura, Y., Yoshinaga, H., Furusawa, M. &Jimi, S. (1981) Elevated cytostatic activity of monocytes from cancer patients.Gann 72, 848–53.PubMedGoogle Scholar
  272. Mason, R. J., Apostolou, S., Power, J. &Robinson, P. (1990) Human alveolar type II cells: stimulation of DNA synthesis by insulin and endothelial cell growth supplement.Am. J. Respir. Cell Mol. Biol. 3, 571–7.PubMedGoogle Scholar
  273. Matsumoto, Y., Kawai, K. &Fujiwara, M. (1988) Hemorrhagic autoimmune encephalomyelitis induced by adoptive transfer of activated semiallogeneic spleen cells into irradiated rats.Am. J. Pathol. 133, 306–15.PubMedGoogle Scholar
  274. Matsumoto, Y., Hanawa, H., Tsuchida, M. &Abo, T. (1993) In situ inactivation of infiltrating T cells in the central nervous system with autoimmune encephalomyelitis. The role of astrocytes.Immunology 79, 381–90.PubMedGoogle Scholar
  275. Matsunaga, S., Ijiri, K., Sakou, T. &Morimoto, N. (1993) Immunohistochemical study of activating osteoblasts from undifferentiated mesenchymal cells by electrical stimulation.Bone 14, 327–32.PubMedCrossRefGoogle Scholar
  276. Matsuno, K., Ezaki, T. &Kotani, M. (1989) Splenic outer periarterial lymphoid sheath (PALS): an immunoproliferative microenvironment constituted by antigen-laden marginal metallophils and ED2-positive macrophages in the rat.Cell Tissue Res. 257, 459–70.PubMedCrossRefGoogle Scholar
  277. Mayron, R. &Wise, D. (1976). Random distribution of centromere regions at mitosis in cultured cells ofMuntiacus muntjak.Chromosoma 55, 69–74.PubMedCrossRefGoogle Scholar
  278. Mazure, A. &Grierson, I. (1992) In vitro studies of the contractility of cell types involved in proliferative vitreoretinopathy.Invest. Ophthalmol. Vis Sci. 33, 3407–16.PubMedGoogle Scholar
  279. Mazzotti, G., Rizzoli, R., Galanzi, A., Papa, S., Vitale, M., Falconi, M., Neri, L. M., Zini, N. &Maraldi, N. M. (1990) Highresolution detection of newly synthesized DNA by antibromodeoxyuridine antibodies identifies specific chromatin domains.J. Histochem. Cytochem. 38, 13–22.PubMedGoogle Scholar
  280. Mcclure, S. J. &Hein, W. R. (1989) Functional characteristics of 197+ CD4- CD8- sheep T lymphocytes: expansion and differentiation of peripheral T cells.Immunol. Cell Biol. 67, 223–31.PubMedGoogle Scholar
  281. Mcculloch, C. A. &Knowles, G. (1991) Discrimination of two fibroblast progenitor populations in early explant cultures of hamster gingiva.Cell Tissue Res. 264, 87–94.PubMedCrossRefGoogle Scholar
  282. Mcdermott, K. W. G. &Lantos, P. L. (1990) Cell proliferation in the subependymal layer of the postnatal marmoset,Callithrix jacchus.Dev. Brain Res. 57, 269–77.CrossRefGoogle Scholar
  283. Mcdowell, E. M., Desanti, A. M. &Strum, J. M. (1989) Correlations between blood glucose levels and bromodeoxyuridine labelling indices of pancreatic islet cells following streptozotocin administration to pregnant Syrian golden hamsters.Virchows Arch. B Cell Pathol. 57, 223–30.Google Scholar
  284. Mcdowell, E. M., Zhang, X. M. &De Santi, A. M. (1990) Localization of healing tracheal wounds using bromodeoxyuridine immunohistochemistry.Stain Technol. 65, 25–9.PubMedGoogle Scholar
  285. Mcnicol, A. M. &Duffy, A. E. (1987) A study of cell migration in the adrenal cortex of the rat using bromodeoxyuridine.Cell Tissue Kinet. 20, 519–26.PubMedGoogle Scholar
  286. Medina, K. L., Smithson, G. &Kincade, P. W. (1993) Suppression of B lymphopoiesis during normal pregnancy.J. Exp. Med. 178, 1507–15.PubMedCrossRefGoogle Scholar
  287. Mencherini, F., Khochbin, S., Chabanas, A. &Lawrence, J.-J. (1992) Seric factors influence cell cycle modifications during commitment of murine erythroleukemia cells to differentiate.Cytometry 13, 518–24.PubMedCrossRefGoogle Scholar
  288. Miething, A. (1993) Proliferative activity of the developing seminiferous epithelium during prespermatogenesis in the golden hamster testis measured by bromodeoxyuridine labelling.Anat. Embryol. (Berl.) 187, 249–58.CrossRefGoogle Scholar
  289. Miller, M. A., Bokhari, S. A., Qadir, K. &Raza, A. (1992) Simultaneous assessment of TGFB and cell cycle kinetics using IUdR/BrdU infusions in human neoplasms from plastic-embedded tissue.J. Histochem. Cytochem. 40, 427–30.PubMedGoogle Scholar
  290. Miller, M. W. (1992) Circadian rhythm of cell proliferation in the telencephalic ventricular zone: effect of in utero exposure to ethanol.Brain Res. 595, 17–24.PubMedCrossRefGoogle Scholar
  291. Miller, M. W. &Nowakowski, R. S. (1988) Use of bromodeoxyuridine-immunohistochemistry to examine the proliferation, migration and time of origin of cells in the central nervous system.Brain Res. 457, 44–52.PubMedCrossRefGoogle Scholar
  292. Minabe, M. (1989) [Regeneration of the rat submandibular glands after duct ligation]Kanagawa Shigaku 24, 484–500.PubMedGoogle Scholar
  293. Miura, M., Domon, M., Sasaki, T. &Takasaki, Y. (1992) Induction of proliferating cell nuclear antigen (PCNA) complex formation in quiescent fibroblasts from a xeroderma pigmentosum patient.J. Cell. Physiol. 150, 370–6.PubMedCrossRefGoogle Scholar
  294. Miyashita, M., Basa, S. &Suzuki, H. (1992) Localized proliferative effect of preadipocytes on cultured human epidermal keratinocytes.Acta Derm. Venereol. (Stockh) 72, 413–17.Google Scholar
  295. Miyata, K., Murao, M., Sawa, M. &Tanishima, T. (1989) [Kinetic study of cell proliferation in a new wound healing model using tissue cultured corneal endothelial cells]Nippon Ganka Gakkai Zasshi 93, 287–93.PubMedGoogle Scholar
  296. Miyata, K., Murao, M., Sawa, M. &Tanishima, T. (1990) New wound-healing model using cultured bovine corneal endothelial cells. 2. Role of migration and mitosis studied by immunohistochemistry.Jpn. J. Ophthalmol. 34, 267–74.PubMedGoogle Scholar
  297. Modlin, I. M., Kumar, R., Nangia, A., Soroka, C. J., Pasikhov, D. &Goldenring, J. R. (1992) Gastrin-dependent inhibitory effects of octreotide on the genesis of gastric ECLomas.Surgery 112, 1048–58.PubMedGoogle Scholar
  298. Moore, G. D., Kopf, G. S. &Schultz, R. M. (1993) Complete mouse egg activation in the absence of sperm by stimulation of an exogenous G protein-coupled receptor.Dev. Biol. 159, 669–78.PubMedCrossRefGoogle Scholar
  299. Moretto, G., Yoo, A. &Kim, S. U. (1993) Human astrocytes and cytokines: tumour necrosis factor alpha and interferon gamma do not promote astrocytic proliferation.Neurosci. Lett. 151, 17–20.PubMedCrossRefGoogle Scholar
  300. Morimoto, Y., Saga, K., Bando, M. &Takahashi, M. (1991) In-vitro DNA synthesis of keratinocytes in normal human skin, psoriasis, seborrhoeic keratosis, Bowen's disease and basal cell carcinoma.Br. J. Dermatol. 125, 9–13.PubMedGoogle Scholar
  301. Morshead, C. M. &Van Der Kooy, D. (1992) Postmitotic death is the fate of constitutively proliferating cells in the subependymal layer of the adult mouse brain.J. Neurosci. 12, 249–56.PubMedGoogle Scholar
  302. Mullenders, L. H., Hazekamp-van Dokkum, A. M., Kalle, W. H., Vrieling, H., Zdzienicka, M. Z. &Van Zeeland, A. A. (1993) UV-induced photolesions, their repair and mutations.Mutat. Res. 299, 271–6.PubMedGoogle Scholar
  303. Munaut, C., Dombrowicz, D. &Matagne, R. F. (1990) Detection of chloroplast DNA by using fluorescent monoclonal anti-bromodeoxyuridine antibody and analysis of its fate during zygote formation inChlamydomonas reinhardtii.Curr. Genet. 18, 259–63.PubMedCrossRefGoogle Scholar
  304. Murakoshi, M., Tagawa, M., Inada, R. &Suzuki, M. (1992) Effects of testosterone, and testosterone plus estrogen, in the castrated rat ventral prostate-histopathological and immunocytochemical studies.Tokai J. Exp. Clin. Med. 17, 133–7.PubMedGoogle Scholar
  305. Murata, T., Wara, R., Abe, H., Kuwabara, N. &Fukuda, Y. (1991) [Studies of morphometric analysis and cell cycle of gastric epithelia of rat administered minimal mixed bile acids].Nippon Shokakibyo Gakkai Zasshi 88, 1532–8.PubMedGoogle Scholar
  306. Murate, T., Saga, S., Hotta, T., Asano, H., Ito, T., Kato, K., Tsushita, K., Kinoshita, T., Ichikawa, A., Yoshida, S. &Saito, H. (1993) The close relationship between DNA replication and the selection of differentiation lineages of human erythroleukemia cell lines K562, HEL, and TF1 into either erythroid or megakaryocytic lineages.Exp. Cell Res. 208, 35–43.PubMedCrossRefGoogle Scholar
  307. Nagai, A., Matsumiya, H., Sakamoto, K., Yamaguchi, E., Aoshiba, K. &Takizawa, T. (1991) [Consequences of DNA damage and the relationship between respiratory diseases in childhood and in adult hood].Nippon Kyobu Shikkan Gakkai Zasshi 29, 7–10.PubMedGoogle Scholar
  308. Nagano, M., Ohkubo, T., Arino, T., Tsuchiya, M. &Nagai, M. (1993) Growth factor for cardiac hypertrophy.Mol. Cell. Biochem. 119, 17–22.PubMedCrossRefGoogle Scholar
  309. Nakamura, H. (1991) Immunohistochemical observations of dividing cells in olfactory epithelium using anti-BrdU antibody.Eur. Arch. Otorhinolaryngol. 248, 298–301.PubMedCrossRefGoogle Scholar
  310. Nakamura, H., Morita, T. &Sato, C. (1986) Structural organizations of replicon domains during DNA synthetic phase in the mammalian nucleus.Exp. Cell Res. 165, 291–7.PubMedCrossRefGoogle Scholar
  311. Nakamura, T. &Hotchi, M. (1992) Changes in DNA strand breaks in non-parenchymal cells following hepatocyte regeneration in CCl4-induced rat liver injury.Virchows Arch. B. Cell Pathol. 63, 11–16.Google Scholar
  312. Nakayasu, H. &Berezney, R. (1989) Mapping replicational sites in the eucaryotic cell nucleus.J. Cell Biol. 108, 1–11.PubMedCrossRefGoogle Scholar
  313. Navone, A. &Raza, A. (1987) The detection of DNA repair in bromodeoxyuridine versus thymidine containing DNA of individual cells.Anticancer Res. 7, 13–16.PubMedGoogle Scholar
  314. Neff, M. W. &Burke, D. J. (1991) Random segregation of chromatids at mitosis inSaccharomyces cerevisiae.Genetics 127, 463–73.PubMedGoogle Scholar
  315. Neri, L. M., Mazzotti, G., Capitani, S., Maraldi, N. M., Cinti, C., Baldini, N., Rana, R. &Martelli, A. M. (1992) Nuclear matrix-bound replicational sites detected in situ by 5-bromodeoxyuridine.Histochemistry 98, 19–32.PubMedCrossRefGoogle Scholar
  316. Niedobitek, G., Finn, T., Herbst, H., Bornhoft, G., Gerdes, J. &Stein, H. (1988) Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes.Am. J. Pathol. 131, 1–4.PubMedGoogle Scholar
  317. Niedobitek, G., Finn, T., Herbst, H. &Stein, H. (1989) Detection of viral genomes in the liver byin situ hybridization using 35S-, bromodeoxyuridine-, and biotin-labeled probes.Am. J. Pathol. 134, 633–9.PubMedGoogle Scholar
  318. Nielsen, J. H., Linde, S., Welinder, B. S., Billestrup, N. &Madsen, O. D. (1989) Growth hormone is a growth factor for the differentiated pancreatic beta-cell.Mol. Endocrinol. 3, 165–73.PubMedGoogle Scholar
  319. Nishino, A., Suzuki, M., Ohtani, H., Motohashi, O., Umezawa, K., Nagura, H. &Yoshimoto, T. (1993) Thrombin may contribute to the pathophysiology of central nervous system injury.J. Neurotrauma 10, 167–79.PubMedGoogle Scholar
  320. Nishizaki, T., Orita, T., Abiko, S., Aoki, H. &Ito, H. (1990) Subependymal giant cell astrocytoma associated with tuberous sclerosis: with special reference to cell kinetic studies—case report.Neurol. Med. Chir. (Tokyo) 30, 695–7.Google Scholar
  321. Nitta, K., Uchida, K., Murai, K., Horita, S., Tsutsui, T., Ozu, H., Kawashima, A., Yumura, W. &Nihei, H. (1993) [Role of glomerular mesangial cells in the regulation of glomerular endothelial cell growth]Nippon Jinzo Gakkai Shi 35, 663–9.PubMedGoogle Scholar
  322. Noll, E. &Miller, R. H. (1993) Oligodendrocyte precursors originate at the ventral ventricular zone dorsal to the ventral midline region in the embryonic rat spinal cord.Development 118, 563–73.PubMedGoogle Scholar
  323. Nomura, A., Maruyama, Y. K. &Yoneda, M. (1991) Initiation of DNA replication cycle in fertilized eggs of the starfish,Asterina pectinifera.Dev. Biol. 143, 289–96.PubMedCrossRefGoogle Scholar
  324. Nomura, A., Yoneda, M. &Tanaka, S. (1993) DNA replication in fertilized eggs of the starfish,Asterina pectinifera.Dev. Biol. 159, 288–97.PubMedCrossRefGoogle Scholar
  325. Ohara, K., Itakura, K. &Ibaraki, N. (1992) Anterior capsule opacification: a cell culture model.Acta Ophthalmol. (suppl.) 29–33.Google Scholar
  326. Ohshio, I. (1992) [Quantitative analysis of DNA synthesis and cell condensation in the oligodactylic rat hand plate]Hokkaido Igaku Zasshi 67, 322–41.PubMedGoogle Scholar
  327. Oinuma, T., Kawano, J-I., &Suganuma, T. (1992) Bromodeoxyuridine-immunohistochemistry on cellular differentiation and migration in the fundic gland ofXenopus laevis during development.Cell Tissue Res. 269, 205–12.PubMedCrossRefGoogle Scholar
  328. Olivo, M., Bhardwaj, R., Schulze-Osthoff, K., Sorg, C., Jacob, H. J. &Flamme, I. (1992) A comparative study on the effects of tumour necrosis factor-alpha (TNF-alpha), human angiogenic factor (h-AF) and basic fibroblast growth factor (bFGF) on the chorioallantoic membrane of the chick embryo.Anat. Rec. 234, 105–15.PubMedCrossRefGoogle Scholar
  329. Orita, T., Nishizaki, T., Kamiryo, T., Harada, K. &Aoki, H. (1988) Cerebral microvascular architecture following experimental cold injury.J. Neurosurg. 68, 608–12.PubMedGoogle Scholar
  330. Orita, T., Akimura, T., Kamiryo, T., Nishizaki, T., Furutani, Y., Harada, K., Ikeyama, Y. &Aoki, H. (1989) Cerebral endothelial regeneration following experimental brain injury. Variation in the regeneration process according to the severity of injury.Acta Neuropathol (Berl.) 77, 397–401.CrossRefGoogle Scholar
  331. Ormerod, M. G., Titley, J. C., Smith, T. A., Tombs, A. L. &Eccles, S. (1993) Proliferative behaviour of an oestrogen sensitive rat mammary tumour: evidence for a paracrine interaction between tumour and stroma.Br. J. Cancer 67, 107–11.PubMedGoogle Scholar
  332. Orton, E. C., La Rue, S. M., Ensley, B. &Stenmark, K. (1992) Bromodeoxyuridine labelling and DNA content of pulmonary arterial medial cells from hypoxia-exposed and nonexposed healthy calves.Am. J. Vet. Res. 53, 1925–30.PubMedGoogle Scholar
  333. Ozawa, S., Ueda, M., Ando, N., Abe, O. &Shimizu, N. (1988) Epidermal growth factor receptors in cancer tissues of esophagus, lung, pancreas, colorectum, breast and stomach.Jpn. J. Cancer Res. 79, 1201–7.PubMedGoogle Scholar
  334. Painter, R. B. &Cleaver, J. E. (1969) Repair replication, unscheduled DNA synthesis and the repair of mammalian DNA.Radiat. Res. 37, 451–66.PubMedGoogle Scholar
  335. Pan, S. A., Okazaki, Y. &Takemoto, T. (1990) In vivo cell kinetics studies of the duodenum during and after healing of cysteamine-induced duodenal ulcer in rats using bromodeoxyuridine incorporation.J. Gastroenterol. Hepatol 5, 281–7.PubMedGoogle Scholar
  336. Patel, S., Rew, D. A., Taylor, I., Potten, C. S., Owen, C. &Roberts, S. A. (1993) Study of the proliferation in human gastric mucosa after in vivo bromodeoxyuridine labelling.Gut 34, 893–6.PubMedGoogle Scholar
  337. Pawlikowski, M., Zerek-Melen, G. &Winczyk, K. (1992) Thyroliberin (TRH) increases thymus cell proliferation in rats.Neuropeptides 23, 199–202.PubMedCrossRefGoogle Scholar
  338. Pawlikowski, M., Zerek-Melen, G., Winczyk, K., Stepien, H., Lachowicz, A. &Janecka, A. (1993) Comparison of the antiproliferative effects of colon mitosis inhibitor, thyroliberin and the somatostatin analog octreotide on rat colonic mucosal epithelial cells.Cytobios 73, 25–30.PubMedGoogle Scholar
  339. Peltomaki, T. &Hakkinen, L. (1992) Growth of the ribs at the costochondral junction in the rat.J. Anat. 181, 259–64.PubMedGoogle Scholar
  340. Penit, C. (1986) In vivo thymocyte maturation. BUdR labelling of cycling thymocytes and phenotypic analysis of their progeny support the single lineage model.J. Immunol. 137, 2115–20.PubMedGoogle Scholar
  341. Penit, C. (1988) Localization and phenotype of cycling and post-cycling murine thymocytes studied by simultaneous detection of bromodeoxyuridine and surface antigens.J. Histochem. Cytochem. 36, 473–8.PubMedGoogle Scholar
  342. Penit, C. &Ezine, S. (1989) Cell proliferation and thymocyte subset reconstitution in sublethally irradiated mice: compared kinetics of endogenous and intrathymically transferred progenitors.Proc. Natl. Acad. Sci. USA 86, 5547–51.PubMedGoogle Scholar
  343. Penit, C. &Vasseur, F. (1988) Sequential events in thymocyte differentiation and thymus regeneration revealed by a combination of bromodeoxyuridine DNA labelling and antimitotic drug treatment.J. Immunol. 140, 3315–23.PubMedGoogle Scholar
  344. Penit, C., Lucas, B. &Vasseur, R. (1995) Cell expansion and growth arrest phases during the transition from precursor (CD4-8-) to immature (CD4+8+) thymocytes in normal and genetically modified mice.J. Immunol. 154, 5103–13.PubMedGoogle Scholar
  345. Perros, P. &Kendall-Taylor, P. (1992) Biological activity of autoantibodies from patients with thyroid-associated ophthalmopathy: in vitro effects on porcine extraocular myoblasts.Q. J. Med. 84, 691–706.PubMedGoogle Scholar
  346. Phillips, R. G. &Whittle, J. R. (1993)wingless expression mediates determination of peripheral nervous system elements in late stages ofDrosophila wing disc development.Development 118, 427–38.PubMedGoogle Scholar
  347. Placzek, M., Jessell, T. M. &Dodd, J. (1993) Induction of floor plate differentiation by contact-dependent, homeogenetic signals.Development 117, 205–18.PubMedGoogle Scholar
  348. Plickert, G. &Kroiher, M. (1988) Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique.Development 103, 791–4.PubMedGoogle Scholar
  349. Poot, M., Hoehn, H., Runger, T. M. &Martin, G. M. (1992) Impaired S-phase transit of Werner syndrome cells expressed in lymphoblastoid cell lines.Exp. Cell Res. 202, 267–73.PubMedCrossRefGoogle Scholar
  350. Potten, C. S., Hume, W. J., Reid, P. &Cairns, J. (1978) Segregation of DNA in stem cells.Cell 15, 899–906.PubMedCrossRefGoogle Scholar
  351. Potten, C. S., Kellett, M., Rew, D. A. &Roberts, S. A. (1992a) Proliferation in human gastrointestinal epithelium using bromodeoxyuridine in vivo: data for different sites, proximity to a tumour, and polyposis coli.Gut 33, 524–9.PubMedGoogle Scholar
  352. Potten, C. S., Kellett, M., Roberts, S. A., Rew, D. A. &Wilson, G. D. (1992b) Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine.Gut 33, 71–8.PubMedGoogle Scholar
  353. Prokop, A. &Technau, G. M. (1991) The origin of postembryonic neuroblasts in the ventral nerve cord ofDrosophila melanogaster.Development 111, 79–88.PubMedGoogle Scholar
  354. Rahman, S. A. &Tsuyama, S. (1993) Immunohistochemical study of cell proliferation and differentiation in epidermis of mice after administration of cholera toxin.Arch. Dermatol. Res. 285, 27–31.PubMedCrossRefGoogle Scholar
  355. Rasmussen, R. E. &Painter, R. B. (1964) Evidence for repair of ultraviolet damaged deoxyribonucleic acid in cultured mammalian cells.Nature 203, 1360–2.PubMedGoogle Scholar
  356. Reddy, K. S., Savage, J. R. &Papworth, D. G. (1988) Replication kinetics of X chromosomes in fibroblasts and lymphocytes.Hum. Genet. 79, 44–8.PubMedCrossRefGoogle Scholar
  357. Regan, J. D. &Setlow, R. B. (1971) Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.Cancer Res. 34, 3318–25.Google Scholar
  358. Reimschuessel, R., Bennett, R. O., May, E. B. &Lipsky, M. M. (1990) Development of newly formed nephrons in the goldfish kidney following hexachlorobutadiene-induced nephrotoxicity.Toxicol. Pathol. 18, 32–8.PubMedGoogle Scholar
  359. Rein, G. &Karasek, M. A. (1992) Factors affecting the growth and maintenance of human skin mast cells in cell culture.Int. Arch. Allergy Immunol. 98, 211–19.PubMedGoogle Scholar
  360. Reitsma, P. H., Rothberg, P. J., Astrin, S. M., Trial, J., Bar-Shavit, Z., Hall, A., Teitelbaum, S. L. &Kahn, A. J. (1983) Regulation of myc gene expression in HL-60 leukemia cells by vitamin D metabolism.Nature 206, 848–50.Google Scholar
  361. Ren, K. Y., Brentjens, J., Chen, Y. X., Brodkin, M. &Noble, B. (1991) Glomerular macrophage proliferation in experimental immune complex nephritis.Clin. Immunol. Immunopathol. 60, 384–98.PubMedCrossRefGoogle Scholar
  362. Repka, A. M. &Adler, R. (1992a) Accurate determination of the time of cell birth using a sequential labelling technique with [3H]-thymidine and bromodeoxyuridine (“window labelling”).J. Histochem. Cytochem. 40, 947–53.PubMedGoogle Scholar
  363. Repka, A. &Adler, R. (1992b) Differentiation of retinal precursor cells born in vitro.Dev. Biol. 153, 242–9.PubMedCrossRefGoogle Scholar
  364. Risio, M., Lipkin, M., Candelaresi, G., Bertone, A., Coverlizza, S. &Rossini, F. P. (1991) Correlations between rectal mucosa cell proliferation and the clinical and pathological features of nonfamilial neoplasia of the large intestine.Cancer Res. 51, 1971–21.Google Scholar
  365. Rizzoli, R., Baratta, B., Maraldi, N. M., Falconi, M., Galanzi, A., Papa, S., Vitale, M., Rizzi, E., Manzoli, L. &Mazzotti, G. (1992). DNA synthesis progression in 3T3 synchronized fibrolasts: a high resolution approach.Histochemistry 97, 181–7.PubMedCrossRefGoogle Scholar
  366. Roberson, D. F., Weisleder, P., Bohrer, P. S. &Rubel, E. W. (1992) Ongoing production of sensory cells in the vestibular epithelium of the chick.Hearing Res. 57, 166–74.CrossRefGoogle Scholar
  367. Robertson, T. A., Maley, M. A., Grounds, M. D. &Papadimitrou, J. M. (1993) The role of macrophages in skeletal muscle regeneration with particular reference to chemotaxis.Exp. Cell Res. 207, 321–31.PubMedCrossRefGoogle Scholar
  368. Rocha, B., Penit, C., Baron, C., Vasseur, F., Dautigny, N. &Freitas, A. A. (1990) Accumulation of bromodeoxyuridine-labeled cells in central and peripheral lymphoid organs: minimal estimates of production and turnover rates of mature lymphocytes.Eur. J. Immunol. 20, 1697–709.PubMedGoogle Scholar
  369. Rode, A., De Taisne, C. &Hartmann, C. (1983) Incorporation of 5-bromodeoxyuridine in the total and ribosomal DNA of synchronously dividing chick embryo fibroblasts.Experientia 39, 1134–6.PubMedCrossRefGoogle Scholar
  370. Rosenstein, B. S. &Rosenstein, R. B. (1992) Survival, excision repair and inhibition of DNA synthesis in normal human skin fibroblasts exposed to simulated sunlight.J. Photochem. Photobiol. B. 16, 247–55.PubMedCrossRefGoogle Scholar
  371. Rosenstein, B. S. &Setlow, R. B. (1980) DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis.Biophys. J. 31, 195–205.PubMedGoogle Scholar
  372. Rosenstein, B. S., Setlow, R. B. &Ahmed, F. E. (1980) Use of the dye Hoechst 33258 in a modification of the bromodeoxyuridine photolysis technique for the analysis of DNA repair.Photochem. Photobiol. 31, 215–22.PubMedGoogle Scholar
  373. Rosette, C. D., De Teresa, P. S., &Pallavicini, M. G. (1990) Simultaneous flow cytometric detection of cellular c-myc protein, incorporated bromodeoxyuridine, and DNA.Cytometry 11, 547–51.PubMedCrossRefGoogle Scholar
  374. Roy, B., Fujimoto, N. &Ito, A. (1990) Growth-promoting effect of retinoic acid in transplantable pituitary tumor of rat.Jpn. J. Cancer Res. 81, 878–83.PubMedGoogle Scholar
  375. Rudulph, N. S. &Latt, S. A. (1989) Flow cytometric analysis of X-ray sensitivity in ataxia telangiectasia.Mutat. Res. 211, 31–41.Google Scholar
  376. Rudolph, N. S., Nagasawa, H., Little, J. B. &Latt, S. A. (1989) Identification of ataxia telangiectasia heterozygotes by flow cytometric analysis of X-ray damage.Mutat. Res. 211, 19–29.PubMedGoogle Scholar
  377. Russev, G. &Boulikas, T. (1992) Repair of transcriptionally active and inactive genes during S and G2 phases of the cell cycle.Eur. J. Biochem. 204, 267–72.PubMedCrossRefGoogle Scholar
  378. Saeter, G., Schwarze, P. E., Nesland, J. M., Juul, N., Pettersen, E. &Seglen, P. O. (1988) The polyploidizing growth pattern of normal rat liver is replaced by divisional, diploid growth in hepatocellular nodules and carcinomas.Carcinogenesis 9, 939–45.PubMedGoogle Scholar
  379. Sakamoto, H., Traincard, F., Vo-Quang, T., Ternynck, T., Guesdon, J. L. &Avrameas, S. (1987) 5-Bromodeoxyuridine in vivo labelling of M13 DNA, and its use as a non-radioactive probe for hybridization experiments.Mol. Cell. Probes 1, 109–20.PubMedCrossRefGoogle Scholar
  380. Sakamoto, S., Mori, T., Singtripop, T., Kawashima, S., Suzuki, S., Kudo, H., Sawaki, K. &Nagasawa, H. (1992) Increase of DNA synthesis in uterine adenomyosis in mice with ectopic pituitary isograft.Acta Anat. (Basel) 145, 162–6.Google Scholar
  381. Sandberg, G. &Ernstrom, U. (1991) In vivo stimulation of thymocyte proliferation by thymocyte growth peptide (TGP).Int. J. Immunopharmacol. 13, 649–54.PubMedCrossRefGoogle Scholar
  382. Sanders, E. J., Prasad, S. &Hu, N. (1993a) The involvement of TGF beta 1 in early avian development: gastrulation and chondrogenesis.Anat. Embryol. (Berl.) 187, 573–81.CrossRefGoogle Scholar
  383. Sanders, E. J., Varedi, M., &French, A. S. (1993b) Cell proliferation in the gastrulating chick embryo: a study using BrdU incorporation and PCNA localization.Development 118, 389–99.PubMedGoogle Scholar
  384. Santavenere, E., Cataldi, A., Rana, R., Vitale, M., Lisio, R., Di Domenicantonio, L., Zamai, L., Trubiani, O. &Miscia, S. (1991) Nuclear metabolic changes induced by tumor necrosis factor in Daudi lymphoma cells; a multiparametric analysis.Cell Biol. Int. Rep. 15, 1235–42.PubMedCrossRefGoogle Scholar
  385. Sapino, A., Macri, L., Gugliotta, P. &Bussolati, G. (1990) Evaluation of proliferating cell types in human and mouse mammary gland by a double immunostaining procedure.Acta Histochem. Suppl. 40, 81–4.PubMedGoogle Scholar
  386. Sasaki, K., Murakami, T., Ogino, T., Takahashi, M. &Kawasaki, S. (1986) Flow cytometric estimation of cell cycle parameters using a monoclonal antibody to bromodeoxyuridine.Cytometry 7, 391–5.PubMedCrossRefGoogle Scholar
  387. Sato, M. &Ahnen, D. J. (1992) Regional variability of colonocyte growth and differentiation in the rat.Anat. Rec. 233, 409–14.PubMedCrossRefGoogle Scholar
  388. Sato, Y., Tsukada, K., Matsumoto, Y. &Abo, T. (1993) Interferongamma inhibits liver regeneration by stimulating major histocompatibility complex class II antigen expression by regenerating liver.Hepatology 18, 3406–9.CrossRefGoogle Scholar
  389. Savage, M. L., Olah, I., &Scott, T. R. (1992) Plasma cell proliferation in the chicken Harderian gland.Cell Prolif. 25, 337–44.PubMedGoogle Scholar
  390. Savelli, V., Rizzoli, R., Rizzi, E., Galanzi, A., Buffa, A., Rana, R., Lattanzi, G. &Baratta, B. (1991) Cell kinetics of vocal fold epithelium in rats.Boll Soc. Ital. Biol. Sper 67, 1081–8.PubMedGoogle Scholar
  391. Saxe, A. &Gibson, G. (1993) Effect of lithium on incorporation of bromodeoxyuridine and tritiated thymidine into human parathyroid cells.Arch. Surg. 128, 865–9.PubMedGoogle Scholar
  392. Scarpini, E., Kreider, B. Q., Lisak, R. P. &Pleasure, D. E. (1988) Establishment of Schwann cell cultures from adult rat peripheral nerves.Exp. Neurol. 102, 167–76.PubMedCrossRefGoogle Scholar
  393. Scarpini, E., Doronzo, R., Baron, P., Moggio, M., Basellini, A. &Scarlato, G. (1992) Phenotypic and proliferative properties of Schwann cells from nerves of diabetic patients.Int. J. Clin. Pharmacol. Res. 12, 211–15.PubMedGoogle Scholar
  394. Scharfmann, R., Basmaciogullari, A. &Czernichow, P. (1990) Effect of growth hormone and glucose on rat islet cells replication using 5-bromo-2-deoxyuridine incorporation.Diabetes Res. 15, 137–41.PubMedGoogle Scholar
  395. Scheres, H. M., Schutte, B., De Goij, A. F. &Bosman, F. T. (1990) Estrogen and progesterone receptor expression and proliferation in breast cancer cells, studied with flow cytometry.Acta Histochem. Suppl.40, 71–6.Google Scholar
  396. Scheven, B. A., Damen, C. A., Hamilton, N. J., Verhaar, H. J. &Duursma, S. A. (1992) Stimulatory effects of estrogen and progesterone on proliferation and differentiation of normal human osteoblast-like cells in vitro.Biochem. Biophys. Res. Commun. 186, 54–60.PubMedCrossRefGoogle Scholar
  397. Schiffer, D., Giordana, M. T., Vigliani, M. C. &Cavalla, P. (1991) Relationship between glial reaction to a stab wound and tumour development after receiving transplacental ethylnitrosourea in the rat.Acta Neuropathol (Berl.) 83, 30–8.CrossRefGoogle Scholar
  398. Schiffer, D., Giordana, M. T., Cavalla, P., Vigliani, M. C. &Attanasio, A. (1993) Immunohistochemistry of glial reaction after injury in the rat: double stainings and markers of cell proliferation.Int. J. Dev. Neurosci. 11, 269–80.PubMedGoogle Scholar
  399. Schiffers, P. M. H., Janssen, G. M. J., Fazzi, G. E., Struijker-Boudier, H. A. J. &De Mey, J. G. R. (1992) Endothelial modulation of DNA synthesis in isolated arteries of the rat.J. Cardiovasc. Pharmacol. 20, Suppl 12, S124–7.Google Scholar
  400. Schindler, D., Kubbies, M., Priest, R. E., Hoehn, H. &Rabinovitch, P. S. (1988) The phytohemagglutinin response of human peripheral blood lymphocytes as a function of donor age: a re-examination using BrdU-Hoechst flow cytometry.Mech. Ageing Dev. 44, 253–63.PubMedCrossRefGoogle Scholar
  401. Schittek, B. &Rajewsky, K. (1990) Maintenance of B-cell memory by long-lived cells generated from proliferating precursors.Nature 346, 749–51.PubMedCrossRefGoogle Scholar
  402. Schittek, B., Rajewsky, K. &Forster, I. (1991) Dividing cells in bone marrow and spleen incorporate bromodeoxyuridine with high efficiency.Eur. J. Immunol. 21, 235–8.PubMedGoogle Scholar
  403. Schmidt, M. &Migeon, B. R. (1990) Asynchronous replication of homologous loci on human active and inactive X chromosomes.Proc. Natl. Acad. Sci. USA 87, 3685–9.PubMedGoogle Scholar
  404. Schultz-Hector, S. &Haghayegh, S. (1993) Beta-fibroblast growth factor expression in human and murine squamous cell carcinomas and its relationship to regional endothelial cell proliferation.Cancer Res. 53, 144–9.Google Scholar
  405. Schutte, B., Reynders, M. M., Bosman, F. T. &Blijham, G. H. (1987) Studies with anti-bromodeoxyuridine antibodies: II. Simultaneous immunocytochemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa.J. Histochem. Cytochem 35, 371–374.PubMedGoogle Scholar
  406. Scippo, M. L., Dombrowicz, D., Igout, A., Closset, J. &Hennen, G. (1989) A non-radioactive method to detect RNA or DNA using an oligonucleotide probe with bromodeoxyuridine free ends, a monoclonal antibody against bromodeoxyuridine and immunogold silver staining.Arch. Int. Physiol. Biochim. 97, 279–84.PubMedGoogle Scholar
  407. Scudiero, D., Henderson, E., Norin, A. &Strauss, B. (1975). The measurement of chemically-induced DNA repair synthesis in human cells by BND cellulose chromatography. Mutat. Res.29, 473–9.PubMedGoogle Scholar
  408. Seifert, R., Zhao, B. &Christ, B. (1992) Cytokinetic studies on the aortic endothelium and limb bud vascularization in avian embryos.Anat. Embryol. (Berl.) 186, 601–10.CrossRefGoogle Scholar
  409. Sejvar, J. J., Landis, S. C. &Hall, A. K. (1993) SA-1 antigen expression in small intensely fluorescent cells is associated with proliferation.Dev. Biol. 157, 547–52.PubMedCrossRefGoogle Scholar
  410. Seki, T. &Arai, Y. (1993) Highly polysialylated neural cell adhesion molecule (NCAM-H) is expressed by newly generated granule cells in the dentate gyrus of the adult rat.J. Neurosci. 13, 2351–8.PubMedGoogle Scholar
  411. Selden, J. R., Dolbeare, F., Clair, J. H., Nichols, W. W., Miller, J. E., Kleemeyer, K. M., Hyland, R. J. &De Luca, J. G. (1993) Statistical confirmation that immunofluorescent detection of DNA repair in human fibroblasts by measurement of bromodeoxyuridine incorporation is stoichiometric and sensitive.Cytometry 14, 154–67.PubMedCrossRefGoogle Scholar
  412. Selden, J. R., Dolbeare, F., Clair, J. H., Miller, J. E., Mcgettigan, K., Dijohn, J. A., Dysart, G. R. &Deluca, J. G. (1994) Validation of a flow cytometricin vitro rat hepatocyte DNA repair (UDS) assay in rat hepatocytes.Mutat. Res. 315, 147–67.PubMedGoogle Scholar
  413. Selig, S., Okumura, K., Ward, D. C. &Cedar, H. (1992) Delineation of DNA replication time zones by fluorescence in situ hybridization.EMBO J. 11, 1217–25.PubMedGoogle Scholar
  414. Selleck, S. B. &Steller, H. (1991) The influence of retinal innervation on neurogenesis in the first optic ganglion ofDrosophila.Neuron 6, 83–99.PubMedCrossRefGoogle Scholar
  415. Selleck, S. B., Gonzalez, C., Glover, D. M. &White, K. (1992) Regulation of the G1-S transition in postembryonic neuronal precursors by axon ingrowth.Nature 355, 253–5.PubMedCrossRefGoogle Scholar
  416. Sellheyer, K., Bickenbach, J. R., Rothnagel, J. A., Bundman, D., Longley, M. A., Krieg, T., Roche, N. S., Roberts, A. B. &Roop, D. R. (1993) Inhibition of skin development by overexpression of transforming growth factor beta 1 in the epidermis of transgenic mice.Proc. Natl. Acad. Sci. USA 90, 5237–41.PubMedGoogle Scholar
  417. Senger, G., Ragoussis, J., Trowsdale, J. &Sheer, D. (1993) Fine mapping of the human MHC class II region within chromosome band 6p21 and evaluation of probe ordering using interphase fluorescence in situ hybridization.Cytogenet. Cell Genet. 64, 49–53.PubMedGoogle Scholar
  418. Seyschab, H., Friedl, R., Schindler, D., Hoehn, H., Rabinovitch, P. S. &Chen, U. (1989) The effects of bacterial lipopolysaccharide, anti-receptor antibodies and recombinant interferon on mouse B cell cycle progression using 5-bromo-2′-deoxyuridine/Hoechst 33258 dye flow cytometry.Eur. J. Immunol. 19, 1605–12.PubMedGoogle Scholar
  419. Sfondrini, G., Gandini, P., Bovari, M., &Bottone, M. G. (1989) [Histological evaluation of tooth movement using immunocytochemical research]Mondo Ortod. 14, 697–702.PubMedGoogle Scholar
  420. Shimizu, A., Tarao, K., Takemiya, S., Harada, M., Inoue, T. &Ono, T. (1988) S-phase cells in diseased human liver determined by an in vitro BrdU-anti-BrdU method.Hepatology 8, 1535–9.PubMedGoogle Scholar
  421. Shinomiya, T. &Ina, S. (1993) DNA replication of histone gene repets inDrosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.Mol. Cell. Biol. 13, 4098–110.PubMedGoogle Scholar
  422. Sieradzan, K. &Vrbova, G. (1989) Replacement of missing motoneurons by embryonic grafts in the rat spinal cord.Neuroscience 31, 115–30.PubMedCrossRefGoogle Scholar
  423. Sieradzan, K. &Vrbova, G. (1993) Replacement of missing motoneurons by embryonic grafts in the rat spinal cord.Neuroscience 31, 115–30.Google Scholar
  424. Sieradzan, K. &Vrbova, G. (1993) Observations on the survival of grafted embryonic motoneurons in the spinal cord of developing rats.Exp. Neurol. 122, 223–31.PubMedGoogle Scholar
  425. Sinha, Y. N. &Sorenson, R. L. (1993) Differential effects of glycosylated and nonglycosylated prolactin on islet cell division and insulin secretion.Proc. Soc. Exp. Biol. Med. 203, 123–6.PubMedGoogle Scholar
  426. Sinkus, A., Wachtler, F. &Schwarzacher, H. G. (1980) Random position of human heterochromatin-bearing chromosomes in first and third mitoses of lymphocyte cultures.Hum. Genet. 54, 125–6.PubMedCrossRefGoogle Scholar
  427. Sinnett, D., Flint, A. &Lalande, M. (1993) Determination of DNA replication kinetics in synchronized human cells using a PCR-based assay.Nuclei Acids Res. 21, 3227–32.Google Scholar
  428. Skaer, H. (1992) Cell proliferation and rearrangement in the development of the Malpighian tubules of the hemipteran,Rhodnius prolixus.Dev. Biol. 150, 372–80.PubMedCrossRefGoogle Scholar
  429. Smith, D. A., Townsend, L. E., Glover, J. L., Seymour, M. L. &Reitz-Vick, D. M. (1992) Atrial myocytes cultured from explanted human adult myocardium demonstrate DNA replication.Am. Surg. 58, 577–82.PubMedGoogle Scholar
  430. Smith, P. J., Leech, C. A. &Treherne, J. E. (1984) Glial repair in an insect central nervous system; effects of selective glial disruption.J. Neurosci. 4, 2698–711.PubMedGoogle Scholar
  431. Smith, P. J., Howe, E. A. &Treherne, J. E. (1987) Mechanisms of glial regeneration in an insect central nervous system.J. Exp. Biol. 132, 59.PubMedGoogle Scholar
  432. Smith, P. J., Howes, E. A. &Treherne, J. E. (1990) Cell proliferation in the repairing adult insect central nervous system: incorporation of the thymidine analogue 5-bromo-2-deoxyuridine in vivo.J. Cell Sci. 95, 599–604.PubMedGoogle Scholar
  433. Sobczak-Thepot, J., Harper, F., Florentin, Y., Zindy, F., Brechot, C. &Puvion, E. (1993) Localization of cyclin A at the sites of cellular DNA replication.Exp. Cell Res. 206, 43–8.PubMedCrossRefGoogle Scholar
  434. Soler, A. P., Laughlin, K. V. &Mullin, J. M. (1993) Effects of epidermal growth factor versus phorbol ester on kidney epithelial (LLC-PK1) tight junction permeability and cell division.Exp. Cell Res. 207, 398–406.PubMedCrossRefGoogle Scholar
  435. Sorscher, D. H., Barrett, J. C. &Cordeiro-Stone, M. (1993) Timing of replication of differentially transcribed genes in Syrian hamster embryo fibroblasts.Mol. Carcinogen. 8, 28–33.Google Scholar
  436. Spack, E. G., Lewis, E. D., Paradowski, B., Schimke, R. T. &Jones, P. P. (1992) Temporal order of DNA replication in the H-2 major histocompatibility complex of the mouse.Mol. Cell. Biol. 12, 5174–88.PubMedGoogle Scholar
  437. Srivastava, V., Miller, S. &Busbee, D. (1993) Immunofluorescent evaluation of DNA repair synthesis using interactive laser cytometry.Cytometry 14, 144–53.PubMedCrossRefGoogle Scholar
  438. Staquet, M. J., De Fraissinette, A., Dezutter-Dambuyant, C., Schmitt, D. &Thivolet, J. (1989) A combined method for detection of cell surface marker expression and bromodeoxyuridine (BrdU) uptake by epidermal cells in suspension.J. Immunol. Methods 116, 287–92.PubMedCrossRefGoogle Scholar
  439. Stenius, U. (1993) Different inhibition of DNA synthesis by transforming growth factor beta and phenobarbital on GST-P-positive and GST-P-negative hepatocytes.Carcinogenesis 14, 159–61.PubMedGoogle Scholar
  440. Stephenson, C. F., Desai, Z. R., &Bridges, J. M. (1991) The proliferative activity of B-chronic lymphocytic leukaemia lymphocytes prior to and after stimulation with TPA and PHA.Leukemia. Res. 15, 1005–12.CrossRefGoogle Scholar
  441. Stevenson, A. P., Crissman, H. A. &Stewart, C. C. (1985) Macrophage-induced cytostasis: kinetic analysis of bromodeoxyuridine-pulsed cells.Cytometry 6, 578–83.PubMedCrossRefGoogle Scholar
  442. Stewart, H. J., Eccleston, P. A., Jessen, K. R. &Mirsky, R. (1991) Interaction between cAMP elevation, identified growth factors, and serum components in regulating Schwann cell growth.J. Neurosci. Res. 30, 346–52.PubMedCrossRefGoogle Scholar
  443. Stofer, W. D. &Horn, J. P. (1993) Neurogenesis and differentiation of sympathetic B and C cells in the bullfrog tadpole.J. Neurosci. 13, 801–7.PubMedGoogle Scholar
  444. Studzinski, G. P. (1989) Oncogenes, growth, and the cell cycle: an overview.Cell Tissue Kinet. 22, 405–24.PubMedGoogle Scholar
  445. Suzuki, Y. &Takeda, M. (1991) Basal cells in the mouse olfactory epithelium after axotomy: immunohistochemical and electron-microscopic studies.Cell Tissue Res. 266, 239–45.PubMedGoogle Scholar
  446. Suzuki, Y. &Takeda, M. (1993) Basal cells in the mouse olfactory epithelium during development: immunohistochemical and electron-microscopic studies.Dev. Brain Res. 73, 107–13.CrossRefGoogle Scholar
  447. Svensson, M. &Aldskogius, H. (1993) Infusion of cytosinearabinoside into the cerebrospinal fluid of the rat brain inhibits the microglial cell proliferation after hypoglossal nerve injury.Glia 7, 286–98.PubMedCrossRefGoogle Scholar
  448. Swales, L. S. &Smith, P. J. (1990) Immunohistochemical localization of the thymidine analogue 5-bromo-2-deoxyuridine in insect tissue: preservation of cellular ultrastructure.Tissue Cell 22, 311–17.PubMedCrossRefGoogle Scholar
  449. Swales, L. S., Howes, E. A. &Smith, P. J. (1992) Cell specific DNA-labelling in the repairing blood-brain barrier of the insectPeriplaneta americana.Cell Tissue Res. 267, 535–43.PubMedCrossRefGoogle Scholar
  450. Tabibzadeh, S. S., Satyaswaroop, P. G. &Rao, P. N. (1988) Antiproliferative effect of interferon-gamma in human endometrial epithelial cells in vitro: potential local growth modulatory role in endometrium.J. Clin. Endocrinol. Metab. 67, 131–8.PubMedGoogle Scholar
  451. Tabibzadeh, S., Bhat, U. G. &Sun, X. (1991) Generation of non-radioactive bromodeoxyuridine labeled DNA probes by polymerase chain reaction.Nucleic Acids Res. 19, 2783.PubMedGoogle Scholar
  452. Tachibana, H., Inoue, S., Oki, H., Andou, H., Katagiri, T. (1993) Sequence in DNA synthetic phase ratio of spontaneous hypertensive rat (SHR) myocardium — study on development of cardiomegaly.Jpn. Circ. J. 57, 442–8.PubMedGoogle Scholar
  453. Takahashi, T., Nowakowski, R. S., &Caviness, V. S., Jr (1992) BUdR as an S-phase marker for quantitative studies of cytokinetic behaviour in the murine cerebral ventricular zone.J. Neurocytol. 21, 185–97.PubMedCrossRefGoogle Scholar
  454. Takahashi, T., Nowakowski, R. S. &Caviness, V. S. Jr (1993) Cell cycle parameters and patterns of nuclear movement in the neocortical proliferative zone of the fetal mouse.J. Neurosci. 13, 820–33.PubMedGoogle Scholar
  455. Takamiya, Y., Kohsaka, S., Toya, S., Otani, M. &Tsukada, Y. (1988) Immunohistochemical studies on the proliferation of reactive astrocytes and the expression of cytoskeletal proteins following brain injury in rats.Brain Res. 466, 201–10.PubMedGoogle Scholar
  456. Takemoto, O., Yoshimine, T., Hayakawa, T., Fujita, T., Nakajima, S., Taneda, M. &Mogami, H. (1989) [Astrocytic proliferation in the brain adjacent to infarcted lesion: immunohistochemical study of astroprotein (GFAP) and bromodeoxyuridine (BrdU)]No To Shinkei 41, 361–5.PubMedGoogle Scholar
  457. Takeno, S., Hamai, Y., Tatsukawa, T., Hamamura, N., Hirakawa, K. &Harada, Y. (1993) [Tissue culture of guinea pig middle ear epithelium-morphological characterization and proliferative activities of cultured cells on fibroblastreorganized collagen gels]Nippon Jibiinkoka Gakkai Kaiho 96, 41–7.PubMedGoogle Scholar
  458. Tanaka, H., Sukhova, G. K., Swanson, S. J. Clinton, S. K., Ganz, P., Cybulsky, M. I. &Libby, P. (1993) Sustained activation of vascular cells and leukocytes in the rabbit aorta after balloon injury.Circulation 88, 1788–804.PubMedGoogle Scholar
  459. Tanaka, N., Tatemoto, A., Urabe, T., Ono, M., Hizuta, A., Naomoto, Y., Gotoh, K., Moreira, L. F. &Orita, K. (1993a) Inhibition of liver regeneration in mice following extended hepatectomy by transfusion of lymphokine activated killer cells.Acta Med. Okayama 47, 21–28.PubMedGoogle Scholar
  460. Tanaka, N., Yamamoto, H., Tatemoto, A., Urabe, T. &Orita, K. (1993b) Regulation of liver regeneration by interleukin-2 and its inhibitors: cyclosporine A and FK 506.Int. J. Immunopharmacol. 15, 211–18.PubMedCrossRefGoogle Scholar
  461. Tanaka, Y., Mak, K. M. &Lieber, C. S. (1990) Immunohistochemical detection of proliferating lipocytes in regenerating rat liver.J. Pathol. 160, 129–34.PubMedCrossRefGoogle Scholar
  462. Tanaka, Y., Funaki, N., Mak, K. M., Kim, C. &Lieber, C. S. (1991a) Effects of ethanol and hepatic vitamin A on the proliferation of lipocytes in regenerating rat liver.J. Hepatol. 12, 344–50.PubMedCrossRefGoogle Scholar
  463. Tanaka, Y., Nouchi, T., Yamane, M., Irie, T., Miyakawa, H., Sato, C. &Marumo, F. (1991b) Phenotypic modulation in lipocytes in experimental liver fibrosis.J. Pathol. 164, 273–8.PubMedCrossRefGoogle Scholar
  464. Tarao, K., Shimizu, A., Harada, M., Kuni, Y., Ito, Y., Tamai, S., Iimori, K., Sugimasa, Y., Takemiya, S., Okamoto, T., Motohashi, H., Sairenji, M., Inoue, T. &Kanisawa, M. (1989) Difference in thein vitro uptake of bromodeoxyuridine between liver cirrhosis with and without hepatocellular carcinoma.Cancer 64, 104–9.PubMedGoogle Scholar
  465. Tarao, K., Shimizu, A., Ohkawa, S., Harada, M., Ito, Y., Tamai, S., Kuni, Y., Nagaoka, T. &Hoshino, H. (1991) Increased uptake of bromodeoxyuridine by hepatocytes from early stage of primary biliary cirrhosis.Gastroenterology 100, 725–30.PubMedGoogle Scholar
  466. Taylor, J. H. (1960) Asynchronous duplication of chromosomes in cultured cells of Chinese hamster.J. Biophys. Biochem. Cytol. 7, 455–64.PubMedGoogle Scholar
  467. Tazzari, P. L., Cipone, M., Tassi, C., Bontadini, A., Cremonini, A. M., Gobbi, M., Gentile, G. &Tura, S. (1989) S-phase evaluation with bromodeoxyuridine in lymphocytes from cattle infected with bovine leukemia virus (BLV).Zentralbl. Veterinarmed. [B] 36, 691–8.Google Scholar
  468. Teragawa, C. K. &Bode, H. R. (1990) Spatial and temporal patterns of interstitial cell migration inHydra vulgaris.Dev. Biol. 138, 63–81.PubMedCrossRefGoogle Scholar
  469. Tezuka, M. (1990) [Cell kinetic study in generating hair tissues in using anti-bromodeoxyuridine monoclonal antibodyl].Nippon Hifuka Gakkai Zasshi 100, 153–61.PubMedGoogle Scholar
  470. Tezuka, M., Ito, M., Ito, K. &Sato, Y. (1990) Cell kinetic study of human and mouse hair tissues using anti-bromodeoxyuridine monoclonal antibody.J. Dermatol. Sci. 1, 335–46.PubMedGoogle Scholar
  471. Tezuka, M., Ito, M., Ito, K., Tazawa, T., &Sato, Y. (1991) Investigation of germinative cells in generating and renewed anagen hair apparatus in mice using anti- bromodeoxyuridine monoclonal antibody.J. Dermatol. Sci. 2, 434–43.PubMedCrossRefGoogle Scholar
  472. Thiry, M. (1988) Immunoelectron microscope localization of bromodeoxyuridine incorporated into DNA of Ehrlich tumour cell nucleoli.Exp. Cell Res. 179, 204–13.PubMedCrossRefGoogle Scholar
  473. Thiry, M. (1992a) Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues.J. Histochem. Cytochem. 40, 411–19.PubMedGoogle Scholar
  474. Thiry, M. (1992a) Ultrastructural detection of DNA within the nucleolus by sensitive molecular immunocytochemistry.Exp. Cell Res. 200, 135–44.PubMedGoogle Scholar
  475. Thiry, M. &Dombrowicz, D. (1988) Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.Biol. Cell 62, 99–102.PubMedCrossRefGoogle Scholar
  476. Tischler, A. S., McClain, R. M., Childers, H. &Downing, J. (1991) Neurogenic signals regulate chromaffin cell proliferation and mediate the mitogenic effect of reserpine in the adult rat adrenal medulla.Lab. Invest. 65, 374–6.PubMedGoogle Scholar
  477. Tischler, A. S., Ruzicka, L. A. &Riseberg, J. C. (1992) Immunocytochemical analysis of chromaffin cell proliferation in vitro.J. Histochem. Cytochem. 40, 1043–5.PubMedGoogle Scholar
  478. Treves, A. J., Barak, V. &Fuks, Z. (1980) Characterization of human lymphocytes which proliferate “spontaneously” in vitro.Eur. J. Immunol. 10, 883–7.PubMedGoogle Scholar
  479. Truman, J. W. &Bate, M. (1988) Spatial and temporal patterns of neurogenesis in the central nervous system ofDrosophila melanogaster.Dev. Biol. 125, 145–57.PubMedCrossRefGoogle Scholar
  480. Tsuji, H., Fuse, Y., Kawamoto, K., Fujino, H. &Kodama, T. (1989) Healing process of experimental esophageal ulcers induced by acetic acid in rats.Scand. J. Gastroenterol. Suppl.162, 6–10.Google Scholar
  481. Tsurushima, H., Yuasa, S., Kawamura, K. &Nose, T. (1993) [Migration of donor Purkinje cells in the host adult rat cerebellum]No To Shinkei 45, 255–62.PubMedGoogle Scholar
  482. Tucker, J. D., Aulett, A., Cimino, M. C., Dearfield, K. L., Jacobsonkram, D., Tice, R. R. &Carrano, A. V. (1993) Sister-chromatid exchange: second report of the Gene-tox Program.Mutat. Res. 297, 101–80.PubMedGoogle Scholar
  483. Usson, Y. &Humbert, C. (1992) Methods for topographical analysis of intra-nuclear BrdUrd-tagged fluorescence.Cytometry 13, 595–602.PubMedCrossRefGoogle Scholar
  484. Uyama, S., Kaufmann, P. M., Takeda, T. &Vacanti, J. P. (1993) Delivery of whole liver-equivalent hepatocyte mass using polymer devices and hepatotrophic stimulation.Transplantation 55, 932–5.PubMedGoogle Scholar
  485. Vambutas, A., Di Lorenzo, T. P. &Steinberg, B. M. (1993) Laryngeal papilloma cells have high levels of epidermal growth factor receptor and respond to epidermal growth factor by a decrease in epithelial differentiation.Cancer Res. 53, 910–14.PubMedGoogle Scholar
  486. van Dekken H., Schervish, E. W., Pizzolo, J. G., Fair, W. R. &Melamed, M. R. (1991) Simultaneous detection of fluorescent in situ hybridization and in vivo incorporated BrdU in a human bladder tumour.J. Pathol. 164, 17–22.PubMedGoogle Scholar
  487. van Dierendonck, J. H., Keijzer, R., Van De Veld, C. J. H. &Cornelisse, C. J. (1989) Subdivision of S-phase by analysis of nuclear 5-bromodeoxyuridine staining patterns.Cytometry 10, 143–50.PubMedGoogle Scholar
  488. Van Dierendonck, J. H., Keijzer, R., Cornelisse, C. J. &Van De Velde, C. J. (1991) Surgically induced cytokinetic responses in experimental rat mammary tumor models.Cancer 68, 759–67.PubMedGoogle Scholar
  489. Van Haaster, L. H., Dejong, F. H., Docter, R. &Derooij, D. G. (1993) High neonatal triiodothyronine levels reduce the period of Sertoli cell proliferation and accelerate tubular lumen formation in the rat testis, and increase serum inhibin levels.Endocrinology 133, 755–60.PubMedGoogle Scholar
  490. Van Noorden, C. J. (1995) Effects of n-3 and n-6 polyunsaturated fatty acid-enriched diets on lipid metabolism in periportal and pericentral compartments of female rat liver lobules and the consequences for cell proliferation after partial hepatectomy.J. Lipid Res. 36, 1708–20.PubMedGoogle Scholar
  491. Vassilev, L. &Johnson, E. M. (1989) Mapping initiation sites of DNA replication in vivo using polymerase chain reaction amplification of nascent strand segments.Nucleic Acids Res. 17, 7693–7712.PubMedGoogle Scholar
  492. Vassilev, L. &Russev, G. (1988) Purification of nascent DNA chains by immunoprecipitation with anti-BrdU antibodies.Nucleic Acids Res. 16, 10397.PubMedGoogle Scholar
  493. Ved Brat, S., Verma, R. S. &Dosik, H. (1981) Sister chromatid differentiation and isolabeling of chromosomes.Hum. Genet. 56, 305–8.Google Scholar
  494. Verstijnen, C. P., Kate, J. T., Arends, J. W., Schutte, B. &Bosman, F. T. (1988) Xenografting of normal colonic mucosa in athymic mice.J. Pathol. 155, 77–85.PubMedCrossRefGoogle Scholar
  495. Vinogradov, A. E., Ezhevsky, S. A., Rosanov, J. M., Kazhdan, I. A. &Zweibach, A. S. (1991) Loosening of cell cycle controls of human lymphocytes under the action of tumour promoter TPA.Cell Prolif. 24, 493–505.PubMedGoogle Scholar
  496. Vitale, M., Rizzoli, R., Mazzotti, G., Zamai, L., Galanzi, A., Rizzi, E., Manzoli, L., Matteucci, A. &Papa, S. (1991) Characterization and cell cycle kinetics of hepatocytes during rat liver regeneration: in vivo BrdUrd incorporation analysed by flow cytometry and electron microscopy.Cell Prolif. 24, 331–8.PubMedGoogle Scholar
  497. Vogel, W. &Speit, G. (1986) Cytogenetic replication studies with short thymidine pulses in bromodeoxyuridine-substituted chromosomes of different mouse cell lines.Hum. Genet. 72, 63–7.PubMedGoogle Scholar
  498. Vogel, W., Boldin, S., Reisacher, A. &Speit, G. (1985) Characterization of chromosome replication during S-phase with bromodeoxyuridine labelling in Chinese hamster ovary and HeLa cells.Chromosoma 92, 363–8.PubMedCrossRefGoogle Scholar
  499. Vogel, W., Autenrieth, M. &Speit, G. (1986) Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns.Hum. Genet. 72, 129–32.PubMedGoogle Scholar
  500. Vogel, W., Autenrieth, M. &Mehnert, K. (1989) Analysis of chromosome replication by a BrdU antibody technique.Chromosoma 98, 335–41.PubMedCrossRefGoogle Scholar
  501. Vogel, W., Mehnert, K. &Pentz, S. (1990) Demonstration of chromosome replication by BrdU antibody technique and electron microscopy.Hum. Genet. 84, 237–40.PubMedCrossRefGoogle Scholar
  502. Vroemen, J. P., Buurman, W. A., Schutte, B., Maessen, J. G., Van Der Linden, C. J. &Kootstra, G. (1988) The cytokinetic behavior of donor hepatocytes after syngenic hepatocyte transplantation into the spleen.Transplantation 45, 600–7.PubMedGoogle Scholar
  503. Wagenaar, G. T. M., Chamuleau, R. A. F. M., Pool, C. W., De Haan, J. G., Maas, M. A. W., Korfage, H. A. M. &Lamers, W. H. (1993) Distribution and activity of glutamine synthase and carbamoylphosphate synthase upon enlargement of the liver lobule by repeated partial hepatectomies.J. Hepatol. 17, 397–407.PubMedCrossRefGoogle Scholar
  504. Wanders, S. L., Ten Kate J., Van Der Linden, E., Derhaag, J., Dinjens, W. N. &Bosman, F. T. (1992) Does ex vivo labelling of proliferating cells in colonic and vaginal mucosa reflect the S-phase fraction in vivo?Histochemistry 98, 267–70.PubMedCrossRefGoogle Scholar
  505. Warchol, M. E., Lambert, P. R., Goldstein, B. J., Forge, A. &Corwin, J. T. (1993) Regenerative proliferation in inner ear sensory epithelia from adult guinea pigs and humans.Science 259, 1619–22.PubMedGoogle Scholar
  506. Ward, J. M., Henneman, J. R., Osipova, G. &Anisimov, V. N. (1991). Persistence of 5-bromo-2′-deoxyuridine in tissues of rats after exposure in early life.Toxicology 70, 345–52.PubMedCrossRefGoogle Scholar
  507. Watanabe, T. &Raff, M. C. (1990) Rod photoreceptor development in vitro: intrinsic properties of proliferating neuroepithelial cells change as development proceeds in the rat retina.Neuron 4, 461–7.PubMedCrossRefGoogle Scholar
  508. Weissman, S. S., Char, D. H., Herbort, C. P., Ostler, H. B. &Kaleta-Michaels, S. (1992) Alteration of human conjunctival epithelial proliferation.Arch. Ophthalmol. 110, 357–9.PubMedGoogle Scholar
  509. Welberg, J. W., De Vries, E. G., Hardonk, M. J., Mulder, N. H., Harms, G., Grond, J., Zwart, N., Koudstaal, J., De Ley, L. &Kleibeuker, J. H. (1990) Proliferation rate of colonic mucosa in normal subjects and patients with colonic neoplasms: a refined immunohistochemical methods.J. Clin. Pathol. 43, 453–6.PubMedGoogle Scholar
  510. Wemme, H., Pfeifer, S., Heck, R. &Muller-Quernheim, J. (1992) Measurement of lymphocyte proliferation: critical analysis of radioactive and photometric methods.Immunobiology 185, 78–89.PubMedGoogle Scholar
  511. Westermann, J., Ronneberg, S., Fritz, F. J. &Pabst, R. (1989a) Proliferation of lymphocyte subsets in the adult rat: a comparison of different lymphoid organs.Eur. J. Immunol. 19, 1087–93.PubMedGoogle Scholar
  512. Westermann, J., Ronneberg, S., Fritz, F. J. &Pabst, R. (1989b) Proliferation of macrophage subpopulations in the adult rat: comparison of various lymphoid organs.J. Leukocyte Biol. 46, 263–9.PubMedGoogle Scholar
  513. Wheeler, J., Robertson, H., Morley, A. R. &Appleton, D. R. (1993) Anti-glomerular basement membrane glomerulonephritis (anti-GBM GN) in the mouse: BrdU-labelling indices and histological damage.Int. J. Exp. Pathol. 74, 9–19.PubMedGoogle Scholar
  514. White, R. A., Fallon, J. F. &Savage, M. P. (1992) On the measurement of cytokinetics by continuous labelling with bromodeoxyuridine with applications to chick wing buds.Cytometry 13, 553–6.PubMedGoogle Scholar
  515. White, S. R., Hershenson, M. B., Sigrist, K. S., Zimmermann, A. &Solway, J. (1993) Proliferation of guinea pig tracheal epithelial cells induced by calcitonin gene-related peptide.Am. J. Respir. Cell Mol. Biol. 8, 592–6.PubMedGoogle Scholar
  516. Wiens, D. J., Mann, T. K., Fedderson, D. E., Rathmell, W. K. &Franck, B. H. (1992) Early heart development in the chick embryo: effects of isotretinoin on cell proliferation, alpha-actin synthesis, and development of contractions.Differentiation 51, 105–12.PubMedGoogle Scholar
  517. Willbold, E. &Layer, P. G. (1992) Formation of neuroblastic layers in chicken retinospheroids: the fibre layer of Chievitz secludes AchE-positive cells from mitotic cells.Cell Tissue Res. 268, 401–8.PubMedCrossRefGoogle Scholar
  518. Wilson, R. G., Smith, A. N. &Bird, C. C. (1990) Immunohistochemical detection of abnormal cell proliferation in colonic mucosa of subjects with polyps.J. Clin. Pathol. 43, 744–7.PubMedGoogle Scholar
  519. Witzig, T. E., Gonchoroff, N. J., Katzmann, J. A., Therneau, T. M., Kyle, R. A. &Greipp, P. R. (1988) Peripheral blood B cell labelling indices are a measure of disease activity in patients with monoclonal gammopathies.J. Clin. Oncol. 6, 1041–6.PubMedGoogle Scholar
  520. Wolf, H. K. &Michalopoulos, G. K. (1992) Hepatocyte regeneration in acute fulminant and nonfulminant hepatitis: a study of proliferating cell nuclear antigen expression.Hepatology 15, 707–13.PubMedGoogle Scholar
  521. Wolf, J. R., Hirschhorn, R. R. &Steiner, S. M. (1992) Growth factor responsiveness: role of MyoD and myogenin.Exp. Cell Res. 202, 105–12.PubMedCrossRefGoogle Scholar
  522. Wood, J. G., Martin, S. &Price, D. J. (1992) Evidence that the earliest generated cells of the murine cerebral cortex form a transient population in the subplate and marginal zone.Dev. Brain Res. 66, 137–40.CrossRefGoogle Scholar
  523. Wurm, F. M., Gwinn, K. A. &Kingston, R. E. (1986) Amplification and inducible overproduction of the mouse c-myc protein in mammalian cells.Proc. Natl. Acad. Sci. USA 83, 5414–18.PubMedGoogle Scholar
  524. Yamaguchi, K., Imanishi, K., Maruno, K. Miyake, Y., Shimosato, &Abe, K. (1989) Lung cancer and autocrine growth factors.Chest 96, 9S-31S.Google Scholar
  525. Yamamoto, M., Fujita, K., Shinkai, T., Yamamoto, K. &Noumura, T. (1992) Identification of the phenotypic modulation of rabbit arterial smooth muscle cells in primary culture by flow cytometry.Exp. Cell Res. 198, 43–51.PubMedCrossRefGoogle Scholar
  526. Yang, G., Matocha, M. F. &Rapoport, S. I. (1990) Increased numbers of extra-adrenal chromaffin cells in the abdominal paraganglia of senescent F344 rats: a possible role for the glucocorticoid receptor.Cell Tissue Res. 259, 233–8.PubMedCrossRefGoogle Scholar
  527. Yao, C. H., Kitazawa, S., Fujimori, T. &Maeda, S. (1993) In situ hybridization at the electron microscopic level using a bromodeoxyuridine labeled DNA probe.Biotech. Histochem. 68, 169–74.PubMedGoogle Scholar
  528. Ye, J., Wang, H. M., Ogden, T. E. &Ryan, S. J. (1993) Allotransplantation of rabbit retinal pigment epithelial cells double-labelled with 5-bromodeoxyuridine (BrdU) and natural pigment.Curr. Eye Res. 12, 629–39.PubMedGoogle Scholar
  529. Yen, A., Samuel, V. &Forbes, M. (1992) Regulation of cell proliferation: late down-regulation of c-myb preceding myelomonocytic cell differentiation.J. Cell. Physiol. 153, 147–56.PubMedCrossRefGoogle Scholar
  530. Yokoi, N., Okamoto, S., Akagi, Y., Terubayashi, H., Ibaraki, N., Tsutsumi, M. &Itoi, M. (1988) [Investigation, of the movement and proliferation of basal cells by BrdU immunohistochemistry in control group of diabetic corneal epitheliopathy]Nippon Ganka Gakkai Zasshi 92, 1112–16.PubMedGoogle Scholar
  531. Yong, V. W., Kim, S. U., Kim, M. W. &Shin, D. H. (1988) Growth factors for human glial cells in culture.Glia 1, 113–23.PubMedCrossRefGoogle Scholar
  532. Yong, V. W., Tejada-Berges, T., Goodyer, C. G., Antel, J. P. &Yong, F. P. (1992) Differential proliferative response of human and mouse astrocytes to gamma-interferon.Glia 6, 269–80.PubMedCrossRefGoogle Scholar
  533. Young, W. G., Zhang, C. Z., Li, H., Osborne, P. &Waters M. J. (1992) The influence of growth hormone on cell proliferation in odontogenic epithelia by bromodeoxyuridine immunocytochemistry and morphometry in the Lewis dwarf rat.J. Dent. Res. 71, 1807–11.PubMedGoogle Scholar
  534. Young, W. G., Zhang, C. Z., Li, H., Lobie, P. E. &Waters, M. J. (1993). A bromodeoxyuridine immunocytochemical and morphometric study of the influence of growth hormone on cell proliferation in odontogenic mesenchyme of the Lewis dwarf rat.Arch. Oral Biol. 38, 207–14.PubMedGoogle Scholar
  535. Yu, A. C., Lee, Y. L. &Eng, L. F. (1993) Astrogliosis in culture: I. The model and the effect of antisense oligonucleotides on glial fibrillary acidic protein synthesis.J. Neurosci. Res. 34, 295–303.PubMedCrossRefGoogle Scholar
  536. Yuasa, K., Arai, T. &Yamada, S. (1990) [Histochemical study of proliferating cells in the spleen in mice with acute hepatic injury].Nippon Shokakibyo Gakkai Zasshi 87, 989–96.PubMedGoogle Scholar
  537. Yuasa, C., Tomita, Y., Shono, M., Ishimura, K. &Ichihara, A. (1993) Importance of cell aggregation for expression of liver functions and regenration demonstrated with primary cultured hepatocytes.J. Cell. Physiol. 156, 522–30.PubMedCrossRefGoogle Scholar
  538. Zelle, B., Berends, F. &Lohman, P. H. (1980) Repair of damage by ultraviolet radiation in xeroderma pigmentosum cell strains of complementation groups E and F.Mutat. Res. 73, 157–69.PubMedGoogle Scholar
  539. Zeymer, U., Fishbein, M. C., Forrester, J. S. &Cercek, B. (1992) Proliferating cell nuclear antigen immunohistochemistry in rat aorta after balloon denudation. Comparison with thymidien and bromodeoxyuridine labelling.Am. J. Pathol. 141, 685–90.PubMedGoogle Scholar
  540. Zhou, T., Bluethmann, H., Eldridge, J., Berry, K. &Mountz, J. D. (1993) Origin of CD4-CD8-B220+ T cells in MRL-lpr/lpr mice. Clues from a T cell receptor beta transgenic mouse.J. Immunol. 150, 3651–67.PubMedGoogle Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Frank Dolbeare
    • 1
  1. 1.Biology and Biotechnology Program, Lawrence Livermore National LaboratoryUniversity of CaliforniaLivermoreUSA

Personalised recommendations