The Histochemical Journal

, Volume 28, Issue 8, pp 531–575 | Cite as

Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites andin situ hybridization

  • Frank Dolbeare


This paper is a continuation of parts I (history, methods and cell kinetics) and II (clinical applications and carcinogenesis) published previously (Dolbeare, 1995Histochem. J.27, 339, 923). Incorporation of bromodeoxyuridine (BrdUrd) into DNA is used to measure proliferation in normal, diseased and injured tissue and to follow the effect of growth factors. Immunochemical detection of BrdUrd can be used to determine proliferative characteristics of differentiating tissues and to obtain birth dates for actual differentiation events. Studies are also described in which BrdUrd is used follow the order of DNA replication in specific chromasomes, DNA replication sites in the nucleus and to monitor DNA repair. BrdUrd incorporation has been used as a tool forin situ hybridization experiments.


Growth Factor Clinical Application Diagnostic Tool Differentiation Event Hybridization Experiment 
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© Chapman & Hall 1996

Authors and Affiliations

  • Frank Dolbeare
    • 1
  1. 1.Biology and Biotechnology Program, Lawrence Livermore National LaboratoryUniversity of CaliforniaLivermoreUSA

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