Chromatographia

, Volume 25, Issue 5, pp 430–436 | Cite as

Indirect detection in reversed-phase liquid chromatography: Ion-pair retention models for ionic compounds on μ Bondapak phenyl

  • E. Arvidsson
  • L. Hackzell
  • G. Schill
  • D. Westerlund
Originals
Received:
Accepted:

Summary

Retention models for ionic compounds in reversed-phase ion-pair chromatographic systems have been developed on the basis of the capacity ratios of solutes and ionic mobile phase components using indirect detection. The binding to the adsorbent, μBondapak Phenyl, has been expressed by ion-pair distribution constants and tested in the retention models by non-linear regression.

All the ionic compounds followed adsorption models of the Langmuir type comprising two kinds of binding sites with widely different adsorption capacities and binding constants. The results indicate certain differences between the two sites regarding the binding of cationic and anionic components, respectively.

Key Words

Ion-pair retention models μBondapak Phenyl Indirect detection 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    A. Tilly-Melin, Y. Askemark, K.-G. Wahlund, G. Schill, Anal. Chem.,51, 976 (1979).CrossRefGoogle Scholar
  2. [2]
    A. Sokolowski, K.-G. Wahlund, J. Chromatogr.,189, 299 (1980).CrossRefGoogle Scholar
  3. [3]
    S. O. Jansson, I. Andersson, B. A. Persson, J. Chromatogr.,203, 93 (1980).Google Scholar
  4. [4]
    A. Tilly-Melin, M. Ljungcrantz, G. Schill, J. Chromatogr.,185, 25 (1979).Google Scholar
  5. [5]
    A. Sokolowski, Chromatographia,22, 168 (1986).Google Scholar
  6. [6]
    A. Sokolowski, T. Fornstedt, D. Westerlund, J. Liq. Chromatogr.10, 1629 (1987).Google Scholar
  7. [7]
    E. Arvidsson, J. Crommen, G. Schill, D. Westerlund, Chromatographia,24, 460 (1987).Google Scholar
  8. [8]
    M. Denkert, L. Hackzell, G. Schill, E. Sjögren, J. Chromatogr.,218, 31 (1981).Google Scholar
  9. [9]
    L. Hackzell, G. Schill, Chromatographia,15, 437 (1982).CrossRefGoogle Scholar
  10. [10]
    M. Schröder-Nielsen, Acta Pharm. Suec.,13, 439 (1976).Google Scholar
  11. [11]
    F. Helfferich, G. Klein, Multicomponent Chromatography, Dekker, New York, 1970.Google Scholar
  12. [12]
    F. Riedo, E. Kovats, J. Chromatogr.,239, 1 (1982).Google Scholar
  13. [13]
    J. Crommen, G. Schill, L. Hackzell, D. Westerlund, Chromatographia,24, 252 (1987).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • E. Arvidsson
    • 1
  • L. Hackzell
    • 1
  • G. Schill
    • 1
  • D. Westerlund
    • 1
  1. 1.Dept. of Analytical Pharmaceutical ChemistryUppsala University Biomedical CenterUppsalaSweden

Personalised recommendations