Qualitative and quantitative analysis of uric acid, creatine and creatinine together with carbohydrates in biological material by HPTLC
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In clinical diagnosis creatine, creatinine and uric acid are important parameters for the evaluation of renal diseases, and are partially responsible for gout and the formation of renal calculi. The determination of various carbohydrates, especially glucose, in urine and serum serves as an indicator of diabetes and other carbohydrate anomalies. A simple, rapid and economic method for the simultaneous screening and quantitation of these compounds is presented. No sample preparation is necessary for the determination in urine or in serum. The proposed method consists of separations on an amino modified HPTLC precoated plate. The detection of all relevant substances is reproducibly performed by simply heating the chromatogram to give stable fluorescent derivatives. The detection limits in all cases lie significantly below the physiological range.
Key WordsThin-layer chromatography Amino modified silica Creatine, creatinine, uric acid and carbohydrates Fluorescence detection In-situ densitometry
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- I.S. Menzies, J.N. Mount, Med. Lab. Technol.32, 269 (1975).Google Scholar
- J.R. Alonso-Fernandez, M.D. Boveda, C. Parrado, J. Pena, J.M. Fraga, J. Chromatogr.217, 357 (1981).Google Scholar
- Z. Zilic, N. Blau, M. Knob, J. Chromatogr.164, 91 (1979).Google Scholar
- M. Winkelmann, W. Schrader, L. Thomas, Medica-Fortbildungskurs 22.-23.11.1978 (Diagnostica Merck company brochure).Google Scholar
- D.N. Buchanan, S.K. Tait, E.F. Domino, J. Chromatogr.163, 212 (1979).Google Scholar
- E. Mussini, L. Colombo, G. De Ponte, F. Marcucci, J. Chromatogr.305, 450 (1984).Google Scholar
- A. Yoshida, Y. Yamaguchi, M. Yoshioka, J. Chromatogr.307, 399 (1984).Google Scholar
- L.E. Webb, J. Chromatogr.381, 406 (1986).Google Scholar
- H.R. Lam, F. Tarding, J. Chromatogr.426, 358 (1988).Google Scholar
- H. Murakita, J. Chromatogr.431, 471 (1988).Google Scholar
- R. Klaus, W. Fischer, in:W.A. Wood, S.T. Kellogg, eds., “Methods in Enzymology”, Vol. 160 Biomass Part A., Academic Press, San Diego, 1988; p. 159.Google Scholar