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Plant Cell, Tissue and Organ Culture

, Volume 1, Issue 1, pp 25–32 | Cite as

Eradication of cassava mosaic disease from Nigerian cassava clones by meristem-tip culture

  • Gabriel O. Adejare
  • Robert H. A. Coutts
Article

Abstract

Methods previously established for the propagation of cassava plants free from cassava mosaic disease have been applied to Nigerian clones. Meristem tips from diseased plants subjected to heat treatment for not less than 30 days at 35°–38°C were cultured on modified Murashige-Skoog medium. Concentration ranges of benzyladenine in combination with α-naphthalene acetic acid and gibberellic acid were investigated and, at optimal levels, 36% of the meristems regenerated. Regenerants, with callus and shoots only, were rooted with 80% efficiency by sub-culturing following a dip in a hormone rooting powder. All plants raised from heat-treated meristems were free of the disease as judged by visual inspection of the leaves, rooted explants and assay for the suspected pathogenic agent of the disease.

Key words

cassava mosaic disease meristem-tip culture 

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References

  1. 1.
    Adejare GO (1981) Nigerian isolates of cassava latent visus. M Phil thesis, University of LondonGoogle Scholar
  2. 2.
    Beck BDA, Chant SR (1958) Tropical Agriculture 35: 59–64Google Scholar
  3. 3.
    Berbee FM, Berbee JG, Hildebrandt AC (1973) In Vitro [Abstr] 8: 421Google Scholar
  4. 4.
    Bock KR, Guthrie EJ (1978) Plant Dis Rep 62: 580–581Google Scholar
  5. 5.
    Chant SR (1959) Emp J of Exp Agric 27: 55–58Google Scholar
  6. 6.
    Kaiser WJ, Teemba LR (1979) Plant Dis Rep 63:780–784Google Scholar
  7. 7.
    Kartha KK, Gamborg OL, Constabel F, Shyluk JP (1974) Plant Sci Lett 2: 107–113Google Scholar
  8. 8.
    Kartha KK, Gamborg OL (1975) Phytopathology 65: 826–828Google Scholar
  9. 9.
    Murashige T, Skoog F (1962) Physiol Plant 15: 473–497Google Scholar
  10. 10.
    Nair NG, Kartha KK, Gamborg OL (1979) Z Pflanzenphysiol 95: 51–56Google Scholar
  11. 11.
    Quak F (1972) In: Proc 10th Int Hort Cong 3: 12–25Google Scholar
  12. 12.
    Shahin EA, Shepard JF (1980) Plant Sci Lett 17: 459–465Google Scholar
  13. 13.
    Storey MH, Nichols RFW (1938) Ann Appl Biol 25: 790–806Google Scholar
  14. 14.
    Zeigler RS, Powell LE, Thurston HD (1980) Phytopathology 70: 589–593Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1981

Authors and Affiliations

  • Gabriel O. Adejare
    • 1
  • Robert H. A. Coutts
    • 1
  1. 1.Plant Pathology Laboratories, The Department of BotanyImperial College of Science and TechnologyLondonUK

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