, Volume 23, Issue 4, pp 292–300 | Cite as

A critical review of some liquid chromatography systems for the separation of sugars

  • M. Verzele
  • G. Simoens
  • F. Van Damme
Review Paper


This contribution to the problems of carbohydrate (sugar) column liquid chromatography, evaluates several currently used systems (reversed phase octadecylated silica gel, amino modified silica gel, cation exchangers on polystyrene and on silica gel basis, polyol derivatised silica gel and anion exchange systems). The elution pattern, analysis time, column efficiency and column life time expectancy are considered to be the important points for this comparison. The application of silica gel-based cation exchanger and of polyol derivatised silica gel is new to this field. The comparison and/or evaluation tries to be critical and to come to a conclusion for the choice of a recommended system.

Key Words

Column liquid chromatography Separation of sugars Review 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    A. Heyraud, M. Rinaudo, J. Liq. Chromatog. Vol. 4 Suppl.2, 175 (1981).Google Scholar
  2. [2]
    K. Robards, M. Whitelaw, J. Chromatog.373, 81 (1986).CrossRefGoogle Scholar
  3. [3]
    J. Di Cesare, Chromatography News Letter Perkin Elmer,8, 52 (1980).Google Scholar
  4. [4]
    L. Verhaar, B. Kuster, H. Claessens, J. Chromatog.284, 1 (1984).CrossRefGoogle Scholar
  5. [5]
    P. Orth, H. Engelhardt, Chromatographia,15, 91 (1982).CrossRefGoogle Scholar
  6. [6]
    M. Boumahraz, V. Davidov, A. Kiselev, Chromatographia,15, 751 (1982).CrossRefGoogle Scholar
  7. [7]
    K. Aitzetmuller, J. Chromatog.156, 354 (1978).Google Scholar
  8. [8]
    B. Wheals, P. White, J. Chromatog.176, 421 (1979).Google Scholar
  9. [9]
    H. Muller, V. Siepe, Chromatographia13, 437 (1980).Google Scholar
  10. [10]
    M. Yang, L. Milligan, G. Mathison, J. Chromatog.209, 316 (1981).CrossRefGoogle Scholar
  11. [11]
    K. Aitzetmuller, Chromatographia13, 432 (1980).Google Scholar
  12. [12]
    P. Shaw, C. Wilson, J. Chromatog. Science20, 209 (1982).Google Scholar
  13. [13]
    C. Brons, C. Olieman, J. Chromatog.259, 79 (1983).CrossRefGoogle Scholar
  14. [14]
    D. Hendrix, R. Lee, J. Baust, H. James, J. Chromatog.210, 45 (1981).CrossRefGoogle Scholar
  15. [15]
    H. Scobell, K. Brobst, J. Chromatog.212, 51 (1981).CrossRefGoogle Scholar
  16. [16]
    L. Verhaar, B. Kuster, J. Chromatog.210, 279 (1980).Google Scholar
  17. [17]
    F. Wentz, A. Marcy, M. Gray, J. Chromatog. Science20, 349 (1982).Google Scholar
  18. [18]
    T. Kawamoto, E. Okada, J. Chromatog.258, 284 (1983).CrossRefGoogle Scholar
  19. [19]
    S. Honda, S. Suzuki, K. Kakehi, J. Chromatog.291, 317 (1984).CrossRefGoogle Scholar
  20. [20]
    J. Schmidt, M. John, C. Wandrey, J. Chromatog.213, 151 (1981).CrossRefGoogle Scholar
  21. [21]
    G. McBee, N. Maness, J. Chromatog.264, 474 (1983).CrossRefGoogle Scholar
  22. [22]
    W. Blaschek, J. Chromatog.256, 157 (1983).CrossRefGoogle Scholar
  23. [23]
    J. Baust, R. Lee, R. Rojas, D. Hendrix, D. Friday, H. James, J. Chromatog.261, 65 (1983).CrossRefGoogle Scholar
  24. [24]
    J. Leonard, F. Guyon, P. Fabiani, Chromatographia18, 600 (1984).CrossRefGoogle Scholar
  25. [25]
    M. Verzele, F. Van Damme, J. Chromatog.362, 23 (1986).Google Scholar
  26. [26]
    C. Lochmuller, W. Hill, J. Chromatog.264, 215 (1983).Google Scholar
  27. [27]
    R. Rocklin, C. Pohl, J. Liq. Chromatog.6, 1577 (1983).Google Scholar
  28. [28]
    K. Mopper, Anal. Biochem.85, 528 (1978).CrossRefGoogle Scholar
  29. [29]
    F. Nevejans, M. Verzele, J. Chromatog. Submitted.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • M. Verzele
    • 1
  • G. Simoens
    • 1
  • F. Van Damme
    • 1
  1. 1.Laboratory of Organic ChemistryState University of GhentGentBelgium

Personalised recommendations