Chromatographia

, Volume 25, Issue 10, pp 875–880

Evaluation of instrumentation used for high performance thin-layer chromatography of aflatoxins

  • R. D. Coker
  • K. Jewers
  • K. I. Tomlins
  • G. Blunden
Originals

Summary

Instrumentation used for high performance thin layer chromatography (HPTLC) of aflatoxins has been evaluated. Optimum sensitivity, accuracy and precision resulted when a combination of a fully automated TLC sampler, an unsaturated conventional TLC glass chamber and a monochromatic densitometer were employed. Benzene: acetonitrile (98: 2 v/v) was found to be the most suitable sample application solvent. These findings are being used to develop a rapid, simple and reproducible method for the analysis of aflatoxins.

Key Words

Thin layer chromatography Sample application Densitometry Development chambers Aflatoxins 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • R. D. Coker
    • 1
  • K. Jewers
    • 1
  • K. I. Tomlins
    • 1
  • G. Blunden
    • 2
  1. 1.Overseas Development Natural Resources InstituteLondonUK
  2. 2.School of Pharmacy & Biomedical SciencesPortsmouth PolytechnicPortsmouthUK

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