Journal of Biological Physics

, Volume 6, Issue 1–2, pp 69–86 | Cite as

Effects of high frequency electric fields on mammalian chromosomes in vitro

  • Michael J. Andrews
  • Joseph A. McClure
Article

Abstract

The effect of an alternating electric field in the frequency range 2 to 50 MHz on human and Chinese hamster chromosomes in liquid suspension was studied. Three distinct types of behavior were observed. Above certain threshold field strengths the chromosomes orient themselves with their long axes along the field direction. The dependence of this threshold on frequency was measured and was found to be much larger at low than at high frequencies. At higher field strengths the chromosomes move together and form end-to-end chains along the field direction. When the field is intense and spacially inhomogeneous, translational motion of the chromosomes occurs. There was no marked difference between the response of the human and hamster chromosomes.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Teixeira-Pinto, A.A., Nejelski, L.L., Cutler, J.L. and Heller, J. H. (1960)Exp. Cell Res. 20, 548–564.Google Scholar
  2. 2.
    Heller, J. H. and Teixeira-Pinto, A.A. (1959)Nature 183, 905–906.Google Scholar
  3. 3.
    Furedi, A.A. and Valentine, R.C. (1962)Biochim. Biophys. Acta 56, 33–42.Google Scholar
  4. 4.
    Furedi, A.A. and Ohad, I. (1964)Biochim. Biophys. Acta 79, 1–8.Google Scholar
  5. 5.
    Sher, L.D. (1963) Mechanical Effects of AC Fields on Particles Dispersed in a Liquid; Biological Implications, Ph.D. thesis. University of Pennsylvania, Philadelphia. University Microfilms No. 63-7086.Google Scholar
  6. 6.
    Schwan, H.P. and Sher, L.D. (1969)J. Electrochem. Soc. 116, 22C-25C.Google Scholar
  7. 7.
    Sher, L.D., Kresch, E. and Schwan, H.P. (1970)Biophysical Jour. 10, 970–979.Google Scholar
  8. 8.
    Sher, L.D. (1968)Nature 220, 695–696.Google Scholar
  9. 9.
    Salzman, N.P., Moore, D.E. and Mendelsohn, J. (1966)Proc. Natl. Acad. Sci. U.S. 56, 1449–1456.ADSGoogle Scholar
  10. 10.
    Moorehead, P.S., Nowell, P.C., Mellman, W.J., Battips, D.M., and Hungerford, D.A. (1960)Exp. Cell Res. 20, 613–620.Google Scholar
  11. 11.
    Technical Information TC Chromosome Culture Kit 5B42.Google Scholar
  12. 12.
    Maio, J.J. and Schildkraut, C.L. (1969)J. Mol. Biol. 40, 203–216.CrossRefGoogle Scholar
  13. 13.
    Saito, M. and Schwan, H.P. (1961) inBiological Effects of Microwave Radiation (Peyton, M.F., ed), Vol. 1, pp. 85–97.Google Scholar
  14. 14.
    Pohl, H.A. (1951)J. Appl. Phys. 22, 869–871.CrossRefGoogle Scholar
  15. 15.
    Krasny-Ergen, W. (1936)Hochfreq. u. Electroak 48, 126–135.Google Scholar
  16. 16.
    Krasny-Ergen, W. (1936)Ann. der Physik 27, 459–464.MATHGoogle Scholar
  17. 17.
    Krasny-Ergen, W. (1937)Hochfreq. u. Electroak 49, 195–201.Google Scholar
  18. 18.
    Saito, M. (1960)A Model for Pearl Chain Formation, Internal Report, The Moore School of Electrical Engineering, University of Pennsylvania.Google Scholar
  19. 19.
    Stratton, J.A. (1941)Electromagnetic Theory, McGraw-Hill, New York, p. 206.Google Scholar
  20. 20.
    Ogawa, T. (1961)J. Appl. Phys. 32, 583–592.CrossRefGoogle Scholar
  21. 21.
    Schwarz, G., Saito, M. and Schwan, H.P. (1965)J. Chem. Phys. 43, 3562–3569.CrossRefGoogle Scholar
  22. 22.
    Landel, A.M., Aloni, Y., Raftery, M.A. and Attardi, G. (1972)Biochem. 11, 1654–1663.Google Scholar
  23. 23.
    Ledley, R.S. and Ruddle, R.H. (1966)Scientific American 214, 40–46.Google Scholar

Copyright information

© Physical Biological Sciences Ltd 1979

Authors and Affiliations

  • Michael J. Andrews
    • 1
  • Joseph A. McClure
    • 1
  1. 1.Department of PhysicsGeorgetown UniversityWashington, D.C.

Personalised recommendations