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Inflammation Research

, Volume 45, Issue 10, pp 494–498 | Cite as

Proteoglycan degrading activity in granulomatous inflammation: Comparison between the C57bl/6 and C57bg/bg mouse

  • D. Prigent
  • M. M. Trancart
  • M. P. Seed
  • D. A. Willoughby
Original Research Papers

Abstract

Objective and Design:

Proteoglycan (GAG) and collagen are lost from cartilage juxtaposed to murine granulomatous tissue in both control and C57bg/bg (elastase deficient mice). The objective was to extract and characterise proteoglycan degrading activity within granulomas of both strains.

Materials:

15 animals (female C57bl/6 and C57bg/bg mice) per group were used.

Treatment:

Cotton-wrapped rat femoral head cartilages were implanted subcutaneously into the dorsum of the mice and the granulomas excised fourteen days later.

Methods:

Granuloma and granuloma cell-granule preparations were fractionated within a detergent-based buffer and tested for their abilities to degrade cartilage in vitro in the presence and absence of enzyme inhibitors. Elastase and cathepsin G activities were also assessed using specific substrates. Statistical significance was calculated using Student's t-test.

Results:

Extracts from both strains induced the loss of cartilage GAG. This was correlated with cathepsin G activity (r=0.96) and was inhibited by a specific cathepsin-G inhibitor (95%, p<0.001), but not specific elastase or metalloproteinase inhibitors. Elastase activity but not that of cathepsin G was absent in the beige mice, whilst both enzymes were active in the controls.

Conclusions:

It appears that neutrophil cathepsin G may play an important role in the degradation of cartilage proteoglycan in the murine cotton-pellet granuloma in both C57bl/6 and C57bg/bg.

Key words

Cartilage degradation model Beige mouse Proteoglycan Cathepsin G Elastase 

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Copyright information

© Birkhäuser Verlag 1996

Authors and Affiliations

  • D. Prigent
    • 1
  • M. M. Trancart
    • 1
  • M. P. Seed
    • 2
  • D. A. Willoughby
    • 2
  1. 1.Laboratoires Jacques LOGEAISTrappesFrance
  2. 2.Experimental PathologySaint Bartholomew's Hospital Medical CollegeLondonUK

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