, Volume 20, Issue 5, pp 289–292 | Cite as

The rapid quantitative analysis of codeine phosphate drug substance by reversed-phase high-performance liquid chromatography

  • W. R. Sisco
  • C. T. Rittenhouse
  • W. M. Maggio


A rapid procedure for the evaluation of codeine phosphate drug substance derived from poppy straw or opium concentrate is described. The gradient HPLC procedure employs a pH 2.5 phosphate buffer, methanol and acetonitrile mobile phase at 40°C and a μBondapak C-18 column. Eight opium alkaloids, including the four major alkaloids, are separated from each other. The paper describes details of the assay procedure and presents data documenting the linearity, specificity, precision, and sensitivity of the method.

Key Words

Column liquid chromatography Reversed-phase Codeine phosphate drug substance Opium alkaloids 


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  1. [1]
    K. Genest, C. G. Farmilo, Anal. Chem.,34, 1964 (1962).CrossRefGoogle Scholar
  2. [2]
    D. J. Reuland, W. A. Trinler, For. Sci.,11, 195 (1978).Google Scholar
  3. [3]
    R. V. D. Rondina, A. L. Bandoni, J. D. Coussio, J. Pharm. Sci.,62, 502 (1973).Google Scholar
  4. [4]
    K. E. Rasmussen, J. Chromatogr.,120, 491 (1976).Google Scholar
  5. [5]
    S. Y. Yeh, J. Pharm. Sci.,62, 1827 (1973).Google Scholar
  6. [6]
    S. J. Mule, Anal. Chem.,36, 1907 (1964).CrossRefGoogle Scholar
  7. [7]
    T. H. Beasley, D. W. Smith, H. W. Ziegler, R. L. Charles, J. Am. Off. Anal. Chem.,57, 85 (1974).Google Scholar
  8. [8]
    P. G. Vincent, B. F. Engelke, J. Am. Off. Anal. Chem.,62, 310 (1979).Google Scholar
  9. [9]
    C. Y. Wu, J. J. Wittick, Anal. Chem.,49, 359 (1977).Google Scholar
  10. [10]
    Y. Nobuhara, S. Hirano, K. Namba, M. Hashimoto, J. Chromatogr.,190, 251 (1980).CrossRefGoogle Scholar
  11. [11]
    I. Lurie, J. Am. Off. Anal. Chem.,60, 1035 (1977).Google Scholar
  12. [12]
    C. Olieman, L. Maat, K. Waleszewski, H. C. Beyerman, J. Chromatogr.,133, 382 (1977).CrossRefGoogle Scholar
  13. [13]
    B. C. Pettitt, C. E. Damon, J. Chromatogr.,242, 189 (1982).CrossRefGoogle Scholar
  14. [14]
    L. W. Doner, A. Hsu, J. Chromatogr.,253, 120 (1982).CrossRefGoogle Scholar
  15. [15]
    W. R. Sisco, R. K. Gilpin, J. Chromatogr. Sci.,18, 41 (1980).Google Scholar
  16. [16]
    R. K. Gilpin, W. R. Sisco, J. Chromatogr.,194, 285 (1980).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1985

Authors and Affiliations

  • W. R. Sisco
    • 1
  • C. T. Rittenhouse
    • 1
  • W. M. Maggio
    • 1
  1. 1.Analytical Development DepartmentMcNeil PharmaceuticalSpring HouseUSA

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