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Antonie van Leeuwenhoek

, Volume 51, Issue 2, pp 167–177 | Cite as

Improvement of sporulation in the yeastYarrowia lipolytica

  • Gerold Barth
  • Herbert Weber
Physiology and Growth
Accepted:

Abstract

Strains ofYarrowia lipolytica forming exclusively spherical ascospores were developed through inbreeding. These strains are more suitable for micromanipulation than other inbred strains forming helm-shaped ascospores. External factors affecting sporulation frequency and tetrad formation in this yeast were investigated. Optimal formation of complete tetrads occurred at a narrow range of pH values around 6.0. Citrate was found to stimulate sporulation strongly. A synthetic medium containing citrate was developed to obtain standard conditions for maximum sporulation.

Keywords

Citrate Standard Condition Narrow Range External Factor Inbred Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Kluwer Academic Publishers 1985

Authors and Affiliations

  • Gerold Barth
    • 1
  • Herbert Weber
    • 1
  1. 1.Akademie der Wissenschaften der DDRForschungszentrum für Molekularbiologie und Medizin, Zentralinstitut für Mikrobiologie und experimentelle TherapieJenaGermany

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