Pharmaceutisch weekblad

, Volume 1, Issue 1, pp 1483–1486 | Cite as

A simple urine-test to prove the intake of disulfiram

  • L. M. L. Stolk
  • P. E. Kamp
Short Communications


Disulfiram is rapidly metabolised in the human body. Therefore the tests, proposed in the literature, always depend upon the detection of one of the metabolites. This paper describes a simple urine-test, based on the detection of the S-glucuronide of diethyldithiocarbamate. Carbon disulphide is liberated from this glucuronide by acid hydrolysis and is then detected by a colorimetric method. The test is positive until 24 hours after oral intake of a normal dose of disulfiram (200–250 mg, once a day).


Disulfiram Diethylamine Reagent Solution Diethyldithiocarbamate Carbon Disulphide 
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  1. Cobby, J., M. Mayersohn andS. Selliah (1977)J. Pharmacol. Exp. Therap. 202, 724–731.Google Scholar
  2. Elenbaas, R. (1977)Am. J. Hosp. Pharm. 34, 827–831.PubMedGoogle Scholar
  3. Kaslander, J. (1963)Biochem. Biophys. Acta 71, 730–732.CrossRefPubMedGoogle Scholar
  4. Kraml, M. (1973)Can. Med. Ass. J. 109, 578.PubMedPubMedCentralGoogle Scholar
  5. Macolm, M., J. Madden andA. Williams (1974)Brit. J. Psychiat. 125, 485–489.CrossRefGoogle Scholar
  6. Massmann, W., andK. Strenge (1956)Arch.Toxicol. 16, 58–63.Google Scholar
  7. Merlevede, E., andH. Casier (1961)Arch. Intern. Pharmacodyn. 132, 427–453.Google Scholar
  8. Neiderhiser, D., R. Fuller, L. Hejduk andH. Roth (1976)J. Chromatog. 117, 187–192.CrossRefGoogle Scholar
  9. Tompsett, S. (1964)Acta Pharmacol. Toxicol. 21, 20–24.CrossRefGoogle Scholar
  10. Strömme, J. (1965)Biochem. Pharmacol. 14, 393–410.CrossRefGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1979

Authors and Affiliations

  • L. M. L. Stolk
    • 1
  • P. E. Kamp
    • 1
  1. 1.Pharmacy department of the University hospital of AmsterdamAmsterdam

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