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Pharmaceutisch weekblad

, Volume 1, Issue 1, pp 695–700 | Cite as

Comparison of the determination of β-lactam antibiotic drugs in plasma of rabbits by means of HPLC and of a microbiological assay

  • Y. A. Hekster
  • A. M. Baars
  • T. B. Vree
  • B. Van Klingeren
  • A. Rutgers
Original Articles

Abstract

The present study compares the determination of the plasma concentrations of ampicillin, amoxycillin and cefuroxime in rabbits, by means of high performance liquid chromatography and the microbiological assay. The correlation coefficient between the data obtained by these methods are for ampicillin 0.991, amoxycillin 0.994 and cefuroxime 0.991.

Plasma constituents interfering with amoxycillin in theHplc determinations, were not encountered in the plasma of man.

Keywords

High Performance Liquid Chromatography Cefuroxime Amoxycillin Netilmicin High Performance Liquid Chromatography Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anhalt, J. P. (1977) Assay of gentamicin in serum by high pressure liquid chromatography,Antimicrob. Agents Chemotherap. II, 651–655.CrossRefGoogle Scholar
  2. Buhs, R. P., T. E. Maxim, N. Allen, T. A. Jacob andF. J. Wolf (1974) Analysis of cefoxitin, cephalothin and their deacylated metabolites in human urine by high performance liquid chromatography,J. Chromatog. 99 609–618.CrossRefGoogle Scholar
  3. Butterfield, A. G., D. W. Hughes, N. J. Pound andW. L. Wilson (1973) Separation and detection of tetracyclines by high speed chromatography,Antimicrob. Agents Chemotherap. 4, 11–15.CrossRefGoogle Scholar
  4. Dalen, R. Van, T. B. Vree, J. C. M. Hafkenscheid andJ. S. F. Gimbrère (1979) Determination of plasma and renal clearance of cefuroxime and its pharmacokinetics in renal insufficiency,Antimicrob. Chemotherap., in press.Google Scholar
  5. Diasio, R. B., M. E. Wilburn, S. Shadomy andA. Espinelingroff (1978) Rapid determination of serum 5-Fluorocytosine levels by High Performance Liquid Chromatography,Antimicrob. Agents Chemotherap. 13, 500–504.CrossRefGoogle Scholar
  6. Lötscher, K. M., B. Brander andH. Kern (1974)Liquid chromatographic analysis of antibiotics. Application note no. 6, Varian.Google Scholar
  7. Maitra, S. K., T. T. Yoshikawa, J. L. Hansen, I. Nilsson-Eh-Le, W. J. Palin, M. C. Schotz andL. B. Guze (1977) Serum gentamicin Assay by High Performance Liquid Chromatography,Clin. Chem. 23, 2275–2278.PubMedGoogle Scholar
  8. Nilsson-Ehle, I. (1977) High Pressure liquid chromatography as a tool for determination of antibiotics in biological fluids,Acta Pathol. Microbiol. Scand, Section B. Supplement259, 61–66.Google Scholar
  9. Nilsson-Ehle, I., T. T. Yoshikawa, M. C. Schotz andL. B. Guze (1978a) Quantitation of antibiotics by High-Pressure Liquid Chromatography: Cephalotin,Antimicrobiol. Agents Chemotherap. 13, 221–227.CrossRefGoogle Scholar
  10. Nilsson-Ehle, I., G. Kahlmeter andP. Nilsson-Ehle (1978b) Determination of chloramphenicol in serum and cerebrospinal fluid with high pressure liquid chromatography,Antimicrobial. Chemother. 4, 169–176.CrossRefGoogle Scholar
  11. Peng, G. W., G. G. Jackson andW. L. Chiou (1977) High-Pressure Liquid chromatography Assay of Netilmicin in plasma,Antimicrobiol. Agents Chemotherap. 12, 707–709.CrossRefGoogle Scholar
  12. Rolewics, T. F., B. L. Mirkin, M. J. Cooper andH. W. Anders (1977) Metabolic disposition of cephalotin and deacetylcephalotin in children and adults: comparison of high performance liquid chromatographic and microbial assay procedures,Clin. Pharmacol. Therap. 22, 928–935.CrossRefGoogle Scholar
  13. Vree, T. B., Y. A. Hekster, A. M. Baars andE. Van Der Kleijn (1978a) Rapid determination of amoxycillin (Clamoxyl®) and ampicillin (Penbritin®) in body fluids of man, by means of high performance liquid chromatography,J. Chromatog. Biomed. Appl. 145, 496–501.CrossRefGoogle Scholar
  14. Vree, T. B., Y. A. Hekster, A. M. Baars, J. E. Damsma andE. Van Der Kleijn (1978b) Determination of trimethoprim and sulfamethoxazole (Co-trimoxazole) in body fluids of man by means of high performance liquid chromatography,J. Chromatog. Biomed. Appl. 146, 103–112.CrossRefGoogle Scholar
  15. Wheeler, L. A., M. De Meo, M. Halula, L. George andP. Heseltine (1978) Use of high-pressure liquid chromatography to determine plasma levels of metronidazole and metabolites after Intravenous administation,Antimicrob. Agents Chemotherap. 13, 205–209.CrossRefGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1979

Authors and Affiliations

  • Y. A. Hekster
    • 1
  • A. M. Baars
    • 1
  • T. B. Vree
    • 1
  • B. Van Klingeren
    • 2
  • A. Rutgers
    • 2
  1. 1.Department of Clinical Pharmacy, St. Radboud HospitalUniversity of NijmegenThe Netherlands
  2. 2.Laboratory of ChemotherapyNational Institute of Public HealthBilthovenThe Netherlands

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