Pharmaceutisch weekblad

, Volume 1, Issue 1, pp 417–424 | Cite as

Determination of tricyclic antidepressants and some of their metabolites in serum by straight phase HPLC

  • D. R. A. Uges
  • P. Bouma
Original Articles


The correlation between blood levels of tricyclic antidepressants and clinical effect is still conflicting. Nevertheless in toxicology, clinical pharmacy and pharmacology a rapid, selective and sensitive assay for these drugs and their metabolites is needed.

AHplc-method is presented for the rapid determination of amitriptyline, nortriptyline, clomipramine, desmethylclomipramine, imipramine, desipramine, doxepin, desmethyldoxepin, protriptyline, trimipramine and maprotiline. The determination is completed within 10 minutes after one single extraction (14 minutes with 10-hydroxynorthriptyline). Using this method a mean coefficient of variation of 3.3% (n=10 per drug, at 100μg/l serum) was found. The determination is easy to combine with an assay for anticonvulsant drugs on the same column (47), and suitable for routine monitoring of the concentration of many major tranquillizers.


Imipramine Amitriptyline Clomipramine Nortriptyline Prussian Blue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Bohn, Scheltema & Holkema 1979

Authors and Affiliations

  • D. R. A. Uges
    • 1
  • P. Bouma
    • 1
  1. 1.Laboratory for Toxicology and Clinical Pharmacy, Department of PharmacyState University HospitalGroningenThe Netherlands

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