Chromatographia

, Volume 27, Issue 1–2, pp 67–70 | Cite as

Evaluation of non-polar bonded-phases for the clean-up of maize extracts prior to aflatoxin assay by HPTLC

  • K. I. Tomlins
  • K. Jewers
  • R. D. Coker
Originals

Summary

The ability of a PH non-polar bonded phase to extract aflatoxin from aqueous solutions (1% v/v) containing 5, 10, 20 and 30% acetone, acetonitrile or methanol was determined. The amount of aflatoxin extracted by the cartridge from aqueous solutions remained almost constant (97–102%) as the concentration of methanol increased, but decreased rapidly when the amount of acetone or acetonitrile increased beyond 10%. Examination of the ability of a number of bonded phases (C2, C8, C18, CH and PH) to extract aflatoxin from aqueous methanol extracts of maize revealed that the PH bonded phase was the most efficient. Aflatoxin is eluted from the cartridge by chloroform (7 ml). An additional clean-up step involving bi-directional development was required when quantifying low levels of aflatoxin in maize by HPTLC.

Key Words

Aflatoxin Non-polar bonded phase extraction cartridges High performance thin layer chromatography 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    R. D. Coker, K. Jewers, K. I. Tomlins, G. Blunden, Chromatographia25, 875 (1988).Google Scholar
  2. [2]
    K. I. Tomlins, K. Jewers, R. D. Coker, M. J. Nagler, Chromatographia666 (1989).Google Scholar
  3. [3]
    L. Stoloff, in “Off. Meth. of Anal. of the Assoc. of Off. Anal. Chem. 14th Ed.”S. Williams, ed., Assoc. Off. Anal. Chem. Inc., Virginia USA, 1984, p. 477.Google Scholar
  4. [4]
    Anon, in “Sorbent Extraction Technol”,K. C. Vanhorne, ed., Analytichem Int. Inc., Harbor City, USA, 1985.Google Scholar
  5. [5]
    M. W. Truckness, W. C. Brumley, N. Stanley, J. Assoc. Off. Anal. Chem.67, 973 (1984).Google Scholar
  6. [6]
    D. L. Orti, R. H. Hill, J. A. Liddle, L. L. Neelham, J. Anal. Toxicol.10, 41 (1986).Google Scholar
  7. [7]
    J. E. Thean, E. R. Lorenz, D. M. Wilson, K. Rodgers, R. C. Gueldner, J. Assoc. Off. Anal. Chem.63, 631 (1980).Google Scholar
  8. [8]
    H. Cohen, M. Lapointe, J. Assoc. Off. Anal. Chem.64, 1372 (1981).Google Scholar
  9. [9]
    W. O. Hurst, K. P. Snyder, R. A. Martin, Peanut Sci.11, 21 (1984).Google Scholar
  10. [10]
    N. Takeda, J. Chromatogr.288, 484 (1984).Google Scholar
  11. [11]
    G. E. Rottinghaus, B. Olesen, G. V. Osweiler, Am. Assn. Vet. Lab. Diagnosticians. 25th Annual Proc. 485 (1982).Google Scholar
  12. [12]
    G. Quan, G. C. Yang, J. Agric. Food Chem.32, 1071 (1984).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • K. I. Tomlins
    • 1
  • K. Jewers
    • 1
  • R. D. Coker
    • 1
  1. 1.Overseas Development Natural Resources InstituteLondonUK

Personalised recommendations