, Volume 27, Issue 1–2, pp 67–70 | Cite as

Evaluation of non-polar bonded-phases for the clean-up of maize extracts prior to aflatoxin assay by HPTLC

  • K. I. Tomlins
  • K. Jewers
  • R. D. Coker


The ability of a PH non-polar bonded phase to extract aflatoxin from aqueous solutions (1% v/v) containing 5, 10, 20 and 30% acetone, acetonitrile or methanol was determined. The amount of aflatoxin extracted by the cartridge from aqueous solutions remained almost constant (97–102%) as the concentration of methanol increased, but decreased rapidly when the amount of acetone or acetonitrile increased beyond 10%. Examination of the ability of a number of bonded phases (C2, C8, C18, CH and PH) to extract aflatoxin from aqueous methanol extracts of maize revealed that the PH bonded phase was the most efficient. Aflatoxin is eluted from the cartridge by chloroform (7 ml). An additional clean-up step involving bi-directional development was required when quantifying low levels of aflatoxin in maize by HPTLC.

Key Words

Aflatoxin Non-polar bonded phase extraction cartridges High performance thin layer chromatography 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • K. I. Tomlins
    • 1
  • K. Jewers
    • 1
  • R. D. Coker
    • 1
  1. 1.Overseas Development Natural Resources InstituteLondonUK

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