, Volume 27, Issue 1–2, pp 49–52 | Cite as

A bi-directional HPTLC development method for the detection of low levels of aflatoxin in maize extracts

  • K. I. Tomlins
  • K. Jewers
  • R. D. Coker
  • M. J. Nagler


A bi-directional high performance thin layer chromatography (HPTLC) method for the determination of low levels of aflatoxin in extracts of maize has been evaluated. The method, using diethylether and chloroform:xylene:acetone (6∶3∶1) as the two developing solvents was found to have a limit of detection of 0.8 (B1), 0.4 (B2), 1.7 (G1) and 0.4 (G2) μg/kg respectivety, with CVs equal or less than 1.7% (B1), 2.5% (B2), 3.1% (G1), and 3.1% (G2). Inter-plate variation was significantly greater than intra-plate variation.

Compared with two-dimensional HPTLC methods, a 15 to 30 fold improvement in the sample capacity per plate is achieved and positioning errors are much reduced during densitometry.

Key Words

Thin layer chromatography Bi-directional development Pre-chromatographic clean-up Aflatoxin 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • K. I. Tomlins
    • 1
  • K. Jewers
    • 1
  • R. D. Coker
    • 1
  • M. J. Nagler
    • 1
  1. 1.Overseas Development Natural Resources InstituteLondonUK

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