Human Genetics

, Volume 97, Issue 5, pp 655–658 | Cite as

Human chromosome 1 localization of the gene for a prostaglandin F, receptor negative regulatory protein

  • David O. Orlicky
  • Rebecca Berry
  • James M. Sikela
Original Investigation

Abstract

A protein that copurifies with the bovine prostaglandin F receptor (FP) has been isolated and the corresponding rat cDNA has been cloned. Transfection experiments suggest that this protein inhibits binding of [3H]prostaglandin F ([3H]PGF) to FP. Histologically, this protein (FP regulatory protein or FPRP) shows a distribution coinciding well with those cells and tissues that respond to PGF. A portion of the 3′ untranslated region of the human homolog to fprp was subcloned, sequenced, and oligonucleotide primers chosen that allow polymerase chain reaction (PCR) amplification specifically of the human fprp sequence. These primers were then used in a PCR-based mapping protocol. The human fprp gene was first localized through human/rodent somatic cell hybrids to human chromosome 1 (100% concordance), and further through yeast artificial chromosome (YAC) pools to region 1p13.1-q21.3 (level 1 mapping). In view of the specific histologic localization of this negative regulator, possible pathological conditions are mentioned that may cosegregate with this chromosomal region.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Berry R, Stevens TJ, Walter NAR, Wilcox AS, Rubano T, Hopkins JA, Weber J, Goold R, Bento Soares M, Sikela JM (1995) Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map. Nat Genet 10:415–423Google Scholar
  2. Boie Y, Rushmore T, Darmon-Goodwin A, Grygorczyk R, Slipetz D, Metters K, Abramovitz M (1994) Cloning and expression of a cDNA for the human prostanoid IP receptor. J Biol Chem 269:12173–12178Google Scholar
  3. Cohen D, Chumakov I, Weissenbach J (1993) A first generation physical map of the human genome. Nature 366:698–701Google Scholar
  4. Coleman RA, Kennedy I, Humphrey PPA, Bunce K, Lumley P (1989) Prostanoids and their receptors. In: Hansch C, et al (eds) Comprehensive medical chemistry, vol 3. Pergamon Press, Oxford, pp 643–714Google Scholar
  5. Duncan AMV, Anderson LL, Funk CD, Abramovitz M, Adam M (1995) Chromosomal localization of the human prostanoid receptor gene family. Genomics 25:740–742Google Scholar
  6. Funk CD (1993) Molecular biology in the eicosanoid field. Prog Nucleic Acid Res Mol Biol 45:67–98Google Scholar
  7. Mendez M, Erickson P, Scott P, Drabkin HA, Gemmill RM (1994) Isolation of YAC clones from genomic libraries by Southern blot hybridization. In: Nelson D, Brownstein B (eds) YAC's: a users guide. Nelson/Freeman, New York, NY, pp 57–92Google Scholar
  8. Moncada S, Flower RJ, Vane JR (1985) Prostaglandins, prostacyclin, thromboxane A2, and leukotrienes. In: Goodman AG, et al (eds) The pharmacological basis of therapeutics, 7th edn. Macmillan, New York, pp 660–673Google Scholar
  9. Niswender GD, Nett TM (1988) The corpus luteum and its control. In: Knobil E, Neill J (eds) The physiology of reproduction. Raven Press, New York, pp 489–525Google Scholar
  10. Orlicky D (1996) Negative regulatory activity of a prostaglandin F receptor associated protein (FPRP). Prostaglandins Leukot Essent Fatty Acids (in press)Google Scholar
  11. Orlicky D, Nordeen S (1996) Cloning, sequencing and proposed structure for a prostaglandin F receptor regulatory protein. Prostaglandins Leukot Essent Fatty Acids (in press)Google Scholar
  12. Orlicky DJ, Miller GJ, Evans RM (1990) Identification and purification of a bovine corpora luteal membrane glycoprotein with [3H]prostaglandin F binding properties. Prostaglandins Leukot Essent Fatty Acids 91:51–61Google Scholar
  13. Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison: Genbank Bionet FASTA search. Proc Natl Acad Sci USA 85:2444–2448Google Scholar
  14. Piper PJ (1973) Distribution and metabolism. In: Cuthbert MF (ed) The prostaglandins: pharmacological and therapeutic advances. Lippincott, Philadelphia, pp 125–150Google Scholar
  15. Robertson RP (1975) Prostaglandins, thromboxanes, and eicosanoids: arachidonic acid metabolites relevant to medicine. Am J Physiol 228:68–70Google Scholar
  16. Smith WL, Marnett LJ, DeWitt DL (1991) Prostaglandin and thromboxane biosynthesis. Pharmacol Ther 49:153–179Google Scholar
  17. Wilcox A, Khan A, Hopkins J, Sikela J (1991) Use of 3′ untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implication for an expression map of the genome. Nucleic Acids Res 19:1837–1843Google Scholar
  18. Wiltbank M, Shiao T, Bergfelt D, Giuther D (1995) Prostaglandin F receptors in the early bovine corpus luteum. Biol Reprod 52:74–78Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • David O. Orlicky
    • 1
  • Rebecca Berry
    • 2
    • 3
  • James M. Sikela
    • 2
    • 3
  1. 1.Department of Pathology and Colorado Cancer CenterUniversity of Colorado Health Sciences CenterDenverUSA
  2. 2.Molecular Biology ProgramUniversity of Colorado Health Sciences CenterDenverUSA
  3. 3.Department of PharmacologyUniversity of Colorado Health Sciences CenterDenverUSA

Personalised recommendations