Bud proliferation and plant regeneration in liquid-cultured philodendron treated with ancymidol and paclobutrazol
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Abstract
Philodendron plants propagated in liquid shake or bioreactor cultures proliferated profusely in the presence of paclobutrazol (PAC) and to a lesser extent in the presence of ancymidol (ANC). The growth retardants inhibited leaf development and induced the formation of bud clusters. Short transient treatments with low concentrations (1.7–3.4 μM) of the growth retardants limited leaf growth and proliferation to a lesser extent than higher concentrations (6.8–17 μM). The growth retardants had a carryover dwarfing effect in the semi-solid hardening medium, which was more pronounced at the higher concentrations or prolonged exposure periods. Regenerated plants resumed normal growth 3–6 weeks after transplanting. Treatment with growth retardants may become a useful method in the prevention of abnormal leaf growth in large-scale liquid cultures, as well as in enhancing bud proliferation.
Keywords
Growth Retardant Paclobutrazol Bioreactor Culture Ancymidol Total Fresh WeightReferences
- Ariel T (1987) The effect of culture condition on development and acclimatization of philodendron ‘Burgundy’ andDianthus caryophyllus. MSc Thesis submitted to the Hebrew University of Jerusalem (In Hebrew with an English summary)Google Scholar
- Ammirato PV, Styer DJ (1985) Strategies for large scale manipulation of somatic embryos in suspension culture. In: Zaitlin M, Day P, Hollander A (eds) Biotechnology in plant science: Relevance to agriculture in the eighties. Academic Press, New York, pp 161–178Google Scholar
- Fletcher RA, Arnold V (1986) Stimulation of cytokinins and chlorophyll synthesis in cucumber cotyledons by triadimefon. Physiol Plant 66:197–201CrossRefGoogle Scholar
- Graebe JE (1987) Gibberellin biosynthesis and control. Ann Rev Plant Physiol 38:419–465CrossRefGoogle Scholar
- Grossmann K (1990). Plant growth retardants as tools in physiological research. Physiol Plant 78:640–648CrossRefGoogle Scholar
- Grossmann K, Hauser C, Sauerbrey E, Fritsch H, Schmidt O, Jung J (1989). Plant growth retardants as inhibitors of ethylene production. J Plant Physiol 134:538–543Google Scholar
- Grossmann K, Kwiatkowski H, Siebecker H, Jung J (1987) Regulation of plant morphology by growth retardants. Effects on phytohormone levels in soybean seedlings determined by immunoassay. Plant Physiol 84:1018–1021PubMedCrossRefGoogle Scholar
- Izumi K, Nakagasa S, Kobayashi M, Oshio H, Sakurai A, Takahashi N (1988) Levels of IAA, cytokinins, ABA and ethylene in rice plants affected by a gibberellin biosynthesis inhibitor, uni-conazole-P. Plant Cell Physiol 29:97–104Google Scholar
- Jung J, Luib M, Sauter H, Zeeh B, Rademacher W (1987) Growth regulation in crop plants with new types of triazole compounds. J Agron Crop Sci 158:324–332CrossRefGoogle Scholar
- Levin R, Gaba V, Tal B, Hirsch S, Denola D, Vasil IK (1988) Automated plant tissue culture for mass propagation. Biotechnology 6:1035–1040CrossRefGoogle Scholar
- Levin R, Vasil IK (1989) Progress in reducing the cost of micropropagation. IAPTC Newsletter 59:473–479Google Scholar
- Luib M, Koehle H, Hoeppnern P, Rademacher W (1987). Further results with BAS 111 04 W, a new growth regulator for use in oilseed rape. Plant growth regulators for agricultural and amenity use. In: Hawkins AF, Stead AD, Pinfield NJ (eds) British Crop Protection Council, Monograph No. 36, Thornton Heath pp 37–43Google Scholar
- Murashige T (1977) Plant cell and organ culture as horticultural practices. Acta Hortic 78:17–30Google Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479CrossRefGoogle Scholar
- Nadel BL, Altman A, Ziv M (1990) Regulation of large scale somatic embryogenesis in celery. Acta Hortic 280:75–82Google Scholar
- Pierik RLM (1990) Commercial micropropagation in Europe. In: Debergh P, Zimmerman R (eds) Micropropagation. Kluwer Academic Publishers, Dordrech (in press)Google Scholar
- Rademacher W (1989) New plant growth retardants: Biochemical background and possibilities for practical application. Acta Hortic 239:477–484Google Scholar
- Steinitz B, Lilien-Kipnis H (1989) Control of precociousGladiolus corm and cormel formation in tissue culture. J Plant Physiol 135:495–500Google Scholar
- Stuart DG, Strickland G, Walker KA (1987) Bioreactor production of alfalfa somatic embryos. Hortscience 22:800–809Google Scholar
- Ziv M (1986)In vitro hardening and acclimatization of tissue culture plants. In: Whithers LA, Alderson PG (eds) Plant tissue culture and its agricultural applications. Butterworths, London, pp 187–196Google Scholar
- Ziv M (1989) Enhanced shoot and cormlet proliferation in liquid culturedGladiolus buds by growth retardants. Plant Cell Tissue Organ Culture 17:101–110CrossRefGoogle Scholar
- Ziv M (1990a) The effect of growth retardants on shoot proliferation and morphogenesis in liquid culturedGladiolus plants. Acta Hortic 280:207–214Google Scholar
- Ziv M (1991b) Morphogenic patterns of plants micropropagated in shaken flasks or large scale bioreactor cultures. Israel J Bot 40:145–153Google Scholar
- Ziv M (1990c) Vitrification. In: Debergh P, Zimmerman R (eds) Micropropagation. Kluwer Academic Press, Durdrecht (in press)Google Scholar
- Ziv M, Hadar A (1991a) Morphogenic pattern ofNephrolepis extalta ‘Bostoniesis’ in agar or liquid cultures—Implication for mass propagation. Israel J Bot 40:7–16Google Scholar
- Ziv M, Yogev T, Krebs O (1986) Effects of paclobutrazol and chlormequat on growth pattern and shoot proliferation of normal and variantAechmea faciata ‘Baker’ plants regeneratedin vitro. Israel J Bot 35:175–182Google Scholar