Calcified Tissue Research

, Volume 2, Issue 1, pp 253–261 | Cite as

Calcium binding by chondroitin sulfate

  • Marshall R. Urist
  • Donald P. Speer
  • Kenneth J. Ibsen
  • Basil S. Strates
Original Papers

Abstract

The binding of calcium by chondroitin sulfate C was studied by employing biological and physicochemical methods for the quantitative determination of ionic calcium. Mean values for logKj, the logarithm of the uncorrected formation constant for a calcium-chondroitin sulfate complex, were 1.64 with the frog heart technique, 1.55 with the murexide method, 1.39 with ultrafiltration and 1.04 with the calcium selective electrode. The magnitude of these values suggests that chondroitin sulfate has a relatively high binding capacity for calcium, but calculated values for an exchange parameterKp indicate that calcium has a low affinity for the polysaccharide.

Key words

Calcium Ion Binding Chondroitin Cartilage 

Résumé

La liaison du calcium par le sulfate de chondroitin C a été étudiée en utilisant des méthodes biologiques et physicochimiques pour la détermination quantitative du calcium ionique. Les valeurs moyennes pour logKj, le logarithme de la constante de formation non corrigée d'un complexe calcium-sulfate de chondroitin C, sont 1,64 par la technique de cœur de grenouille, 1,55 par la méthode de murexide, 1,39 par l'ultrafiltration et 1,04 par l'électrode sélective du calcium. L'ordre de grandeur de ces valeurs suggèrent que le sulfate de chondroitin C a une capacité de liaison pour calcium relativement élevée, mais les valeurs calculées pour un paramètre d'échangeKp indiquent que le calcium a une affinité faible pour le polysaccharide.

Zusammenfassung

Die Bindung von Calcium mit Chondroitinsulfat C wurde untersucht mittels biologischer und physikalisch-chemischer Methoden für die quantitative Bestimmung des ionischen Calciums. Im Durchschnitt ergab logKj, der Logarithmus der unkorrigierten Formationskonstante für einen Calciumchondroitinsulfatkomplex, 1,64 mit der Technik des Froschherzens, 1,55 mit der Murexidmethode, 1,39 mittels Ultrafiltration und 1,04 mit den Calciumselektiven Elektroden. Die Größe dieser Werte zeigt, daß Chondroitinsulfat eine relativ hohe Bindungskapazität für Calcium hat, die berechneten Werte für einen AustauschparameterKp jedoch, daß Calcium eine kleine Affinität für Polysaccharide hat.

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Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • Marshall R. Urist
    • 1
  • Donald P. Speer
    • 1
  • Kenneth J. Ibsen
    • 1
  • Basil S. Strates
    • 1
  1. 1.Bone Research Laboratory, School of MedicineUniversity of CaliforniaLos Angeles

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