Molecular Breeding

, Volume 1, Issue 3, pp 295–301

Seed specific expression of the 2S albumin gene from Brazil nut (Bertholletia excelsa) in transgenicVicia narbonensis

  • Thomas Pickardt
  • Isolde Saalbach
  • David Waddell
  • Martin Meixner
  • Klaus Müntz
  • Otto Schieder
Short Communication

DOI: 10.1007/BF02277429

Cite this article as:
Pickardt, T., Saalbach, I., Waddell, D. et al. Mol Breeding (1995) 1: 295. doi:10.1007/BF02277429

Abstract

In order to evaluate the possibility of enhancing the sulphur amino acids in grain legumes, we have transferred the gene for the methionine-rich 2S albumin from Brazil nut (Bertholletia excelsa) controlled by a seed-specific promoter intoVicia narbonensis. The coding region of the 2S albumin gene that has been completely synthesized was fused to the seed-specific leguminB4 promoter fromVicia faba. Transgenic lines ofV. narbonensis were generated viaAgrobacterium-mediated gene analysis of leaves, stems, roots and seeds of four R0 plants revealed that the expression of the foreign 2S albumin gene occurred in a seed-specific manner and that the foreign protein is present at levels ranging from 1% to 4.8% of total SDS-soluble seed protein. Since the current protocol for transformation ofV. narbonensis has not yet been published, a detailed description of the method is given.

Key words

Brazil nut protein, grain legumes, leguminB4 promoter seed storage protein sulphur amino acid deficiency transgenic plants Vicia narbonensis 

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Thomas Pickardt
    • 1
  • Isolde Saalbach
    • 2
  • David Waddell
    • 2
  • Martin Meixner
    • 1
  • Klaus Müntz
    • 2
  • Otto Schieder
    • 1
  1. 1.Institut für Angewandte GenetikFreie Universität BerlinBerlinGermany
  2. 2.Institut für Pflanzengenetik und KulturpflanzenforschungGaterslebenGermany

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