Chromatographia

, Volume 39, Issue 11–12, pp 673–681 | Cite as

High-speed chiral separation of beta-blockers by supercritical fluid chromatography on ChyRoSine-A

  • N. Bargmann-Leyder
  • D. Thiébaut
  • F. Vergne
  • A. Bégos
  • A. Tambuté
  • M. Caude
Article
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Summary

High-speed separation of β-blockers have been successfully obtained (analysis time < 2 min) using a short column packed with ChyRoSine-A CSP. Peculiar chromatographic behaviour of β-blockers on tyrosinederived CSPs under supercritical conditions was evidenced by studying the curves of hversus the linear velocity u.

Specific solvation of solutes by supercritical carbon dioxide is responsible for the particular conformation of the molecular of β-blocker which enhances chiral discrimination. This was evidenced by using the reciprocality concept leading to a new CSP derived from a propranolol analogue. Thus, it was demonstrated that SFC can be used for the fast chiral separation of β-blockers.

Key Words

Supercritical fluid chromatography ß-Blockers Solvation with supercritical CO2 New chiral phase Chiral separation Chiral resolution 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • N. Bargmann-Leyder
    • 1
  • D. Thiébaut
    • 1
  • F. Vergne
    • 2
  • A. Bégos
    • 2
  • A. Tambuté
    • 2
  • M. Caude
    • 1
  1. 1.Laboratoire de Chimie Analytique de l'Ecole Supérieure de Physique et Chimie Industrielles de ParisUnité de recherche No. 437 associée au CNRS)Paris Cedex 05France
  2. 2.Centre d'Etudes du BouchetVert-le-PetitFrance

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