Pharmaceutisch Weekblad

, Volume 2, Issue 1, pp 833–839 | Cite as

Novel Chromatographic methods for monitoring quaternary ammonium compounds in biological fluids

  • J. E. Greying
  • J. H. G. Jonkman
  • H. G. M. Westenberg
  • R. A. De Zeeuw


Three novel Chromatographic methods for the determination of quaternary ammonium compounds with biomedical applications are presented and their sensitivity, selectivity, reproducibility and applicability for different groups of the compounds are compared.

The first one is a gas Chromatographie method for those compounds with three identical short-chain alkyl groups attached to the nitrogen atom and describes a reproducible dealkylation in the injection port of the gas Chromatograph to their respective tertiary amines. An alkali-flame ionisation detector is used as a sensitive and selective detector.

The second one is a method for quaternary ammonium compounds with an ester function and is based on hydrolysis and derivatisation of the resulting acids with pentafluorobenzyl bromide, followed by gas chromatography with electron capture detection.

The third method is based on ion-pair adsorption high performance liquid chromatography by adding an inorganic counter-ion to the mobile phase.


High Performance Liquid Chromatography Nitrogen Atom Alkyl Group Electron Capture Biological Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Bohn, Scheltema & Holkema 1980

Authors and Affiliations

  • J. E. Greying
    • 1
  • J. H. G. Jonkman
    • 1
  • H. G. M. Westenberg
    • 1
  • R. A. De Zeeuw
    • 1
  1. 1.Department of Toxicology, Laboratory for Pharmaceutical and Analytical ChemistryState Universityaw GroningenThe Netherlands

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