Human Genetics

, Volume 94, Issue 1, pp 91–94

A human cDNA coding for the Leydig insulin-like peptide (Ley I-L)

  • E. Burkhardt
  • I. M. Adham
  • U. Hobohm
  • D. Murphy
  • C. Sander
  • W. Engel
Short Communication

Abstract

cDNA clones for the human Leydig insulin-like peptide (Ley I-L) have been isolated and characterized. The nucleotide sequence of the 743-bp cDNA includes an incomplete 7-bp 5′-noncoding region, an open reading frame of 393 bp, and a 343-bp 3′-noncoding region. By primer extension analysis, the transcription start site was determined as being 14-bp upstream of the translation start site. The underlying gene is expressed in the testis but not in other organs. From the cDNA sequence, it can be deduced that the Ley I-L protein is synthesized as a 131-amino-acid (aa) preproprotein and that it contains a 24-aa signal peptide. Comparison of the pro Ley I-L protein with members of the insulin-like hormone superfamily predicts that the biologically active hormone, after proteolytic processing of the C peptide, consists of a 31-aa long B chain and a 26-aa long A chain, and that it has a molecular weight of 6.25 kDa.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • E. Burkhardt
    • 1
  • I. M. Adham
    • 1
  • U. Hobohm
    • 2
  • D. Murphy
    • 1
  • C. Sander
    • 2
  • W. Engel
    • 1
  1. 1.Institut für Humangenetik der Universität GödttingenGöttingenGermany
  2. 2.European Molecular Biology Laboratory HeidelbergGermany

Personalised recommendations