Summary
Phencyclidine (PCP) was found to be extractable by headspace solid-phase microextraction (SPME) from human whole blood and urine. Sample solutions were heated at 90°C in the presence of NaOH and K2CO3, and an SPME fiber was exposed in the headspace of a vial for 30 min. Immediately after withdrawal of the fiber, it was analyzed by gas chromatography with surface ionization detection (GC-SID). Recoveries of PCP were approximately 9.3–10.8% and 39.8–47.8% for whole blood and urine samples, respectively. The calibration curve for PCP showed good linearity in the range 2.5–100 ng mL−1 whole blood and 0.5–100 ng mL−1 urine. The detection limits were approximately 1.0 ng mL−1 for whole blood and 0.25 ng mL−1 for urine.
References
J. H. Jaffe, in “The Pharmacological Basis of Therapeutics”, A. G. Gilman, T. W. Rall, A. S. Nies, P. Taylor, eds., Pergamon Press, New York, 1991, p. 557.
S. R. Zukin, R. S. Zukin, in “Substance Abuse: A Comprehensive Textbook”, J. H. Lowinson, P. Ruiz, R. B. Millman, eds., William & Willkins, Baltimore, 1992, p. 290.
C. Arthur, J. Pawliszyn, Anal. Chem.62, 2165 (1990).
S. B. Hawthorne, D. J. Miller, J. Pawliszyn, C. L. Arthur, J. Chromatogr.603, 185 (1992).
D. J. Potter, J. Pawliszyn, J. Chromatogr.625, 247 (1992).
B. D. Page, G. Lacroix, J. Chromatogr.648, 199 (1993).
T. Kumazawa, X.-P. Lee, M.-C. Tsai, H. Seno, A. Ishii, K. Sato, Jpn J. Forensic Toxicol.13, 25 (1995).
X.-P. Lee, T. Kumazawa, K. Sato, Int. J. Legal Med.107, 310 (1995).
T. Kumazawa, X.-P. Lee, K. Sato, H. Seno, A. Ishii, O. Suzuki, Jpn J. Forensic Toxicol.13, 182 (1995).
T. Kumazawa, K. Watanabe, K. Sato, H. Seno, A. Ishii, O. Suzuki, Jpn J. Forensic Toxicol.13, 207 (1995).
H. Seno, T. Kumazawa, A. Ishii, M. Nishikawa, H. Hattori, O. Suzuki, Jpn J. Forensic Toxicol.13, 211 (1995).
M. Yashiki, N. Nakagawa, T. Kojima, T. Miyazaki, Y. Iwasaki, Jpn J. Forensic Toxicol.13, 17 (1995).
T. Fujii, H. Arimoto, Anal. Chem.57, 2625 (1985).
T. Fujii, H. Arimoto, in “Detectors for capillary Chromatography”, Chemical Analysis Series Vol. 121, H. H. Hill, D. G. McMinn, eds., John Wiley & Sons, Chichester, 1992, p. 169.
D. O. Clardy, R. H. Cravey, B. J. MacDonald, S. J. Wiersema, D. S. Pearce, J. L. Reagle, J. Anal. Toxicol.3, 238 (1979).
A. C. Moffat, J. V. Jackson, M. S. Moss, B. Windrop, eds., “Clarke's Isolation and Identification of Drugs”, Pharmaceutical Press, London, 1986, p. 874.
A. Ishii, H. Seno, T. Kumazawa, M. Nishikawa, K. Watanabe, O. Suzuki, Int. J. Legal Med.,108, 244 (1996).
D. Louch, S. Mortlaugh, J. Pawliszyn, Anal. Chem.64, 1187 (1992).
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Ishii, A., Seno, H., Kumazawa, T. et al. Simple extraction of phencyclidine from human body fluids by headspace solid-phase microextraction (SPME). Chromatographia 43, 331–333 (1996). https://doi.org/10.1007/BF02271005
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DOI: https://doi.org/10.1007/BF02271005