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Chromatographia

, Volume 41, Issue 9–10, pp 581–584 | Cite as

Separation of saponins and determination of quinovic acid 3-O-α-L-rhamnopyranoside fromNauclea diderrichii (de Wild) Merr. Bark by high performance liquid chromatography

  • M. Lamidi
  • T. Martin-Lopez
  • E. Ollivier
  • F. Crespin-Maillard
  • L. Nze Ekekang
  • G. Balansard
Originals

Summary

A reversed-phase HPLC determination of 11 saponins and an alkaloid glycoside inNauclea diderrichii bark is described. Quantitative analysis of quinovic acid 3-O-α-L-rhamnopyranoside which is one of the main saponins was determined, using Chrysanthelline A as internal standard. The complete separation was achieved in 47 minutes. The method of quantification was validated.

Key Words

Column liquid chromatography Nauclea diderrichii (de Wild) Merr Saponins Alkaloid glycoside Chrysanthelline A 

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References

  1. [1]
    A. Walker, R. Silans, “Les plantes utiles du Gabon”, Paul Lechevalier, Paris, 1961, p. 373.Google Scholar
  2. [2]
    A. Bouquet, “Feticheurs et Medecines traditionnelles du Congo” (Brazzaville), ORSTOM, Paris, 1969, p. 212.Google Scholar
  3. [3]
    S. McLean, D. G. Murray, Can. J. Chem.48, 867 (1970).Google Scholar
  4. [4]
    S. McLean, D. G. Murray, Can. J. Chem.50, 1496 (1972).Google Scholar
  5. [5]
    M. Lamidi, E. Ollivier, R. Faure, L. Debrauwer, L. Nze-Ekekang, G. Balansard, Pharma. and Pharmaco. Letters5, 1, 8 (1995).Google Scholar
  6. [6]
    M. Lamidi, E. Ollivier, R. Faure, L. Debrauwer, L. Nze-Ekekang, G. Balansard, Phytochem.38, 1, 209 (1995).Google Scholar
  7. [7]
    M. Lamidi, E. Ollivier, R. Faure, L. Debrauwer, L. Nze-Ekekang, G. Balansard, Planta Medica61, 280 (1995)Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1995

Authors and Affiliations

  • M. Lamidi
    • 1
    • 2
    • 3
  • T. Martin-Lopez
    • 2
  • E. Ollivier
    • 1
  • F. Crespin-Maillard
    • 1
  • L. Nze Ekekang
    • 3
  • G. Balansard
    • 1
  1. 1.Laboratory of PharmacognosyFaculty of PharmacyMarseille Cedex 5France
  2. 2.Laboratory of PharmacognosyFaculty of PharmacyMadridSpain
  3. 3.IPHAMETRA (Cenarest)LibrevilleGabon

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