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Chromatographia

, Volume 34, Issue 5–8, pp 235–240 | Cite as

Determination of partition coefficients by automatic equilibrium headspace gas chromatography by vapor phase calibration

  • B. Kolb
  • C. Welter
  • C. Bichler
Article

Summary

Equilibrium headspace gas chromatography has been applied to the determination of the partition coefficients of volatile compounds in water-air systems. Only techniques that are suited to a fully automatic headspace procedure using the pneumatic headspace sampling-technique have been considered. Particularly simple is the technique of “vapor phase calibration —VPC” where an external vapor standard is used to calibrate the concentration of the volatile analyte in the headspace, while the concentration in the sample is found from the difference in the total amount in the vial. This technique is described in detail for 2-butanone in water. Finally, the water-air partition coefficients of several selected volatile compounds at different temperatures are listed together with their temperature functions.

Key Words

Gas chromatography Headspace sampling Partition coefficients Multiple headspace extraction 

List of Symbols and Abbreviations

AC

Peak area of analyte from external vapor standard

AS

Peak area of analyte from headspace sample

aC

slope of regression of analyte from external vapor standard

aS

slope of regression of analyte from sample vial

CS

Concentration of analyte in sample

CG

Concentration of analyte in gas phase of sample vial

CGC

Vapor concentration of analyte in external vapor standard

K

Partition coefficient

mo

Total amount of analyte in vial

mG

Amount of analyte in gas phase

mS

Amount of analyte in sample

Pa

Atmospheric pressure

Ph

Headspace pressure in pressurized vial (Ph=Pa+PGC) with PGC as excess pressure given by pressure of GC

VG

Volume of gas phase (headspace) in vial

VS

Volume of sample in vial

VV

Volume of headspace vial

β

Phase ratio (β=VG/VS)

@

Calibration factor

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References

  1. [1]
    L.S. Ettre, B. Kolb, Chromatographia32, 5 (1991).Google Scholar
  2. [2]
    L. Rohrschneider, Anal. Chem.45, 1241 (1973).Google Scholar
  3. [3]
    W.J. Jones, M.J. Egoville, E.O. Strolle, E.S. Dellamonica, J. Chromatogr.455, 45 (1988).Google Scholar
  4. [4]
    T.G. Kieckbusch, C.J. King, J. Chromatogr. Sci.17, 273 (1978).Google Scholar
  5. [5]
    C.A. McAuliffe, Chem Tech, 46–51, Jan. 1971.Google Scholar
  6. [6]
    A.G. Vitenberg, B.V. Ioffe, Z.S. Dimitrova, I.L. Butaeva, J. Chromatogr.112, 319 (1975).Google Scholar
  7. [7]
    B.V. Ioffe, A.G. Vitenberg, Head-Space Analysis and Related Methods in Gas Chromatography. John Wiley & Sons, New York, NY, 1984.Google Scholar
  8. [8]
    R. Guitart, A. Puigdemont, M. Arboix, J. Chromatogr.491, 271 (1989).Google Scholar
  9. [9]
    P. Pospisil, B. Kolb, paper presented at the “Anwender-Kolloquim über die Gaschromatographische Dampfraum-analyse”, Bad Nauheim, 3.–4. October 1983.Google Scholar
  10. [10]
    B. Kolb, L.S. Ettre, Chromatographia32, 505 (1991).Google Scholar
  11. [11]
    B. Kolb, J. Chromatogr.,112, 287 (1975).Google Scholar
  12. [12]
    B. Kolb, in: B. Kolb (Ed.), Applied Headspace Gas Chromatograpy. Heyden & Son Ltd., New York, NY, pp. 1–11 (1980).Google Scholar
  13. [13]
    K. Schoene, J. Steinhanses, A. König, J. Chromatogr.455, 57 (1988).Google Scholar
  14. [14]
    N. Onda, A. Shinohara, H. Ishii, A. Sato, HRC&CC14, 357 (1991).Google Scholar
  15. [15]
    P.E. Porter, C.H. Deal, F.H. Stross, J. Am Chem. Soc.78, 2999 (1956).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1992

Authors and Affiliations

  • B. Kolb
    • 1
  • C. Welter
    • 1
  • C. Bichler
    • 1
  1. 1.Bodenseewerk Perkin-Elmer GmbHÜberlingenGermany

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