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Two intragenic polymorphisms of the APC-gene detected by PCR and enzymatic digestion

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Summary

The gene causing adenomatous polyposis coli (APC) has recently been cloned. Three intragenic polymorphisms were reported to be detectable by single-strand conformation polymorphism analysis. Here, we describe an assay using polymerase-chain-reaction-based amplification and subsequent enzymatic digestion of genomic sequences to identify polymorphic sites at nucleotide positions 1458 and 5037 of the APC gene.

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References

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Author information

Correspondence to Wolfgang G. Ballhausen.

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Kraus, C., Ballhausen, W.G. Two intragenic polymorphisms of the APC-gene detected by PCR and enzymatic digestion. Hum Genet 88, 705–706 (1992). https://doi.org/10.1007/BF02265305

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Keywords

  • Nucleotide
  • Internal Medicine
  • Genomic Sequence
  • Metabolic Disease
  • Enzymatic Digestion