Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Assignment of the gene encoding the catalytic subunit Cβ of CAMP-dependent protein kinase to the p36 band on chromosome

Summary

A cDNA for the human catalytic subunit (Cβ) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for Cβ as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kbXbaI fragment, which hybridized with the Cβ probe, was concordant with the presence of human chromosome 1.In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the Cβ gene of PKA to the p36 band on chromosome 1.

This is a preview of subscription content, log in to check access.

References

  1. Adavani SR, Schwarz M, Showers MO, Maurer RA, Hemmings BA (1987) Multiple mRNA species code for the catalytic subunit of the cAMP-dependent protein kinase from LLC-PK1 cells. Eur J Biochem 167:221–226

  2. Bale SJ, Dracopoli NC, Tucker MA, Clark WH Jr, Fraser MC, Stanger BZ, Green P, Donis-Keller H, Housman DE, Greene MH (1989) Mapping the gene for hereditary cutaneous malignant melanoma-dysplastic nevus to chromosome 1p. N Engl J Med 320:1367–1372

  3. Beebe SJ, Corbin JD (1986) Cyclic nucleotide-dependent protein kinases. In: Boyer PD, Krebs EG (eds) The enzymes, vol 17A. Academic Press, Orlando London, p 43–111

  4. Beebe SJ, Øyen O, Sandberg M, Frøysa A, Hansson V, Jahnsen T (1990) Molecular cloning of a tissue-specific protein kinase (C7) from human testis — representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. Mol Endocrinol 4:465–475

  5. Bérubé D, Gagne R (1990) Exonuclease digestion of human chromosomes for in situ hybridization and R-banding. Nucleic Acids Res 18:2831

  6. Bérubé D, The VL, Lachance Y, Gagne R, Labrie F (1989) Assignment of the human 3β-hydroxysteroid dehydrogenase gene (HSDB3) to the p13 band of chromosome 1. Cytogenet Cell Genet 52:199–200

  7. Boyd E, Theriault A, Goddard JP, Kalaitsidaki M, Spathas DH, Connor JM (1989) Chromosomal assignment of a glutamic acid transfer RNA (tRNA-Glu) gene to 1p36. Hum Genet 81:153–156

  8. Boyle JM, Hey Y, Kessel AG van, Fox M (1988) Assignment of ecto-5′ nucleotidase to human chromosome 6. Hum Genet 81:88–92

  9. Boyle JM, Hey Y, Fox M (1989) Nucleotide ecto-enzyme activities of human and chinese hamster fibroblast in tissue culture. Biochem Genet 27:655–671

  10. Buckland RA (1989) Genomic organization of the human asparagine transfer RNA genes: localization to the U1 RNA gene and class I pseudogene repeat units. Am J Hum Genet 45:283–295

  11. Clegg CH, Cadd GG, McKnight GS (1988) Genetic characterization of a brain specific form of the type I regulatory subunit of cAMP-dependent protein kinase. Proc Natl Acad Sci USA 85:3703–3707

  12. Corbin JD, Keely SL, Park CR (1975) The distribution and dissociation of cyclic adenosine 3′:5′-monophosphate-dependent protein kinases in adipose, cardiac and other tissues. J Biol Chem 150:218–225

  13. Dracopoli NC, Harnett P, Bale SJ, Stanger BZ, Tucker MA, Housman DE, Kefford RF (1989) Loss of alleles from the distal short arm of chromosome 1 occurs late in melanoma tumor progression. Proc Natl Acad Sci USA 86:4614–4618

  14. Edelman AM, Blumenthal DK, Krebs EG (1987) Protein serine threonine kinases. Annu Rev Biochem 56:567–613

  15. Fong CT, Dracopoli NC, White PS, Merrill PT, Griffith R, Housman DE, Brodeur GM (1989) Loss of heterozygosity for the short arm of chromosome 1 in human neuroblastomas: correlation with N-myc amplification. Proc Natl Acad Sci USA 86:3753–3757

  16. Grzeschik K-H (1973) Syrian hamster-human somatic cell hybrids: isolation and characterization. Humangenetik 20:211–218

  17. Grzeschik K-H (1976) Assignment of a structural gene for betaglucoronidase to human chromosome 7. Somat Cell Genet 2:401–410

  18. Grzeschik K-H, Mayerova A, Bender K, Johannesmann R (1978) Regional submapping of human chromosome 6 by the use of human-mouse somatic cell hybrids. Cytogenet Cell Genet 22:412–414

  19. Jahnsen T, Hedin L, Kidd VJ, Beattie WG, Lohmann SM, Walter U, Durica J, Schulz TJ, Schiltz E, Browner M, Goldman D, Ratoosh SL, Richards JS (1986) Molecular cloning, cDNA structure and regulation of the regulatory subunit (RII51) of type II cAMP-dependent protein kinase from rat granulosa cells. J Biol Chem 261:12352–12361

  20. Jobs A, Klein-Bölting D, Jandel AS, Driesel A, Olek K, Grzeschik K-H (1990) Regional assignment of 41 human DNA fragments on chromosome 7 by means of a somatic cell hybrid panel. Hum Genet 84:147–150

  21. Lee DC, Carmichael DF, Krebs EG, McKnight GS (1983) Isolation of a cDNA clone for the type I regulatory subunit of bovine cAMP-dependent protein kinase. Proc Natl Acad Sci USA 80:3605–3612

  22. Lindgren V, Bernstein LB, Weiner AM, Francke U (1985) Human U1 small nuclear RNA pseudogenes do not map to the site of the Ul genes in 1p36 but are clustered in 1q12-q22. Mol Cell Biol 5:2172–2180

  23. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

  24. Mathew CGP, Smith BA, Thorpe K, Wong Z, Royle NJ, Jeffreys AJ, Ponder BAJ (1987) Deletion of genes on chromosome 1 in endocrine neoplasia. Nature 328:524–526

  25. Mattei MG, Sardet C, Franchi A, Pouyssegur J (1988) The human amiloride-sensitive Na+/H+ antiporter: localization to chromosome 1 by in situ hybridization. Cytogenet Cell Genet 48:6–8

  26. Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158

  27. Povey S, Morton NE, Sherman SK (1985) Report of the committee on the genetic constitution of chromosomes 1 and 2. Cytogenet Cell Genet 40:67–106

  28. Sakaguchi AY, Zabel BU, Grzeschik K-H, Law ML, Ellis RW, Scolnick EM, Naylor SL (1984) Regional localization of two human cellular Kirsten ras genes on chromosome 6 and 12. Mol Cell Biol 4:989–993

  29. Saksela K, Bergh J, Lehto VP, Nilsson K, Alitalo K (1985) Amplification of the c-myc oncogene in a subpopulation of human small cell lung cancer. Cancer Res 45:1823–1827

  30. Scott JD, Glaccum MB, Zoller MJ, Uhler MD, Helfman DM, McKnight GS, Krebs EG (1987) The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain. Proc Natl Acad Sci USA 84:5186–5192

  31. Showers MO, Maurer RA (1986) A cloned bovine cDNA encodes an alternate form of the catalytic subunit of cAMP-dependent protein kinase. J Biol Chem 261:16288–16291

  32. Smith M, Weiss MJ, Griffin CA, Murray JC, Buetow KH, Emanuel BS, Henthorn PS, Harris H (1988) Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1–p34. Genomics 2:139–143

  33. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

  34. Taylor SS (1989) cAMP-dependent protein kinase-model for an enzyme family. J Biol Chem 264:8443–8446

  35. Tronick SR, Popescu NC, Cheah MSC, Swan DC, Amsbaugh SC, Lengel CR, DiPaolo JA, Robbins KC (1985) Isolation and chromosomal localization of the human for protooncogene, a distinct member of the tyrosine kinase gene family. Proc Natl Acad Sci USA 82:6595–6599

  36. Uhler MD, Carmichael DF, Lee DC, Chrivia JC, Krebs EG, McKnight GS (1986a) Isolation of cDNA clones coding for the catalytic subunit of mouse cAMP-dependent protein kinase. Proc Natl Acad Sci USA 83:1300–1304

  37. Uhler MD, Chrivia JC, McKnight GS (1986b) Evidence for a second isoform of the catalytic subunit cAMP-dependent protein kinase. J Biol Chem 261:15360–15363

  38. Yang-Feng TL, Floyd-Smith G, Nemer M, Drouin J, Francke U (1985) The pronatriodilation gene is located on the distal short arm of human chromosome 1 and on mouse chromosome 4. Am J Hum Genet 37:1117–1128

  39. Zoller MJ, Kerlavaga A, Taylor SS (1979) Structural comparisons of cAMP-dependent protein kinase I and II from porcine skeletal muscle. J Biol Chem 150:218–225

Download references

Author information

Correspondence to Tore Jahnsen.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Simard, J., Bérubé, D., Sandberg, M. et al. Assignment of the gene encoding the catalytic subunit Cβ of CAMP-dependent protein kinase to the p36 band on chromosome. Hum Genet 88, 653–657 (1992). https://doi.org/10.1007/BF02265292

Download citation

Keywords

  • Protein Kinase
  • Blot Analysis
  • Metabolic Disease
  • Somatic Cell
  • cDNA Library