Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Plasma levels of insulin, glucagon and glucagon-like peptide in salmonids of different weights

  • 61 Accesses

  • 36 Citations

Abstract

Plasma levels of insulin in rainbow trout,Oncorhynchus mykiss, Atlantic salmon,Salmo salar, and Pacific coho salmon,Oncorhynchus kisutch and plasma circulating levels of glucagon and glucagon-like peptide, in rainbow trout and Atlantic salmon, were measured by homologous radioimmunoassays. Hormonal levels were compared against the average body weight of the same group of fish. Plasma insulin levels were significantly correlated (r=0.56, 0.46 and 0.42 respectively) with body weight in all three salmonid species. Moreover, rainbow trout from fast-growing families had significantly higher (p<0.005) plasma insulin levels than did fish from slow-growing families. Plasma titres of glucagon and glucagon-like peptide were always lower than insulin titres and did not correlate with body weight.

This is a preview of subscription content, log in to check access.

References cited

  1. Ablett, R.F., Sinnhuber, R.O. and Selivonchick, D.P. 1981a. The effect of bovine insulin on (14C)glucose and (3H)leucine incorporation in fed and fasted rainbow trout (Salmo gairdneri). Gen. Comp. Endocrinol. 44: 418–427.

  2. Ablett, R.F., Sinnhuber, R.O., Holmes, R.M. and Selivonchick D.P. 1981b. The effect of prolonged administration of bovine insulin in rainbow trout (Salmo gairdneri R.) Gen. Comp. Endocrinol. 43: 211–217.

  3. Bassas, L., Lesniak, M.A., Girbau, M. and De Pablo, F. 1987. Insulin-related receptors in the early chick embryo: from tissue patterns to possible functions. J. Exp. Zool., Suppl. 1: 299–307.

  4. Beguinot, F., Kahn, C.R., Moses, A.C. and Smith, R.J. 1985. Distinct biologically active receptors for insulin, insulin-like growth factor I, and insulin-like growth factor-II in cultured skeletal muscle cells. J. Biol. Chem. 260: 15892–15898.

  5. Bern, H.A. 1978. Endocrinological studies on normal and abnormal salmon smoltification.In Comparative Endocrinology. pp. 97–100. Edited by P.J. Gaillard and H.H. Boer. Elsevier/North Holland Biomedial Press, Amsterdam.

  6. Cahill, G.F. Jr., Aoki, T.T. and Smith, R.J. 1983. Glucagon and amino acids metabolism.In Handbook of Experimental Pharmacology Vol. 66/1, Glucagon. pp. 399–417. Edited by P.J. Lefébvre. Springer-Verlag, Berlin.

  7. Christiansen, D.C. and Klungsøyr, L. 1987. Metabolic utilization of nutrients and the effects of insulin in fish. Comp. Biochem. Physiol. 88B: 701–711.

  8. Donaldson, E.M., Fagerlund, U.H.M., Higgs, D.A. and McBride, J.R. 1979. Hormonal enhancement of growth.In Fish Physiology. Vol. 8, pp. 455–597. Edited by W.S. Hoar, D.J. Randall and R.J. Brett. Academic Press, New York.

  9. Duronio, V. and Jacobs, S. 1988. Comparison of insulin and IGF-1 receptors.In Receptor Biochemistry and Methodology. Vol. 12B, Insulin Receptors, Part B. pp. 3–18. Edited by C.R. Kahn and L.C. Harrison. Alan R. Liss, Inc., New York.

  10. Ensinck, J.W. 1983. Immunoassays for glucagon.In Handbook of Experimental Pharmacology Vol. 66/1. pp. 203–221. Edited by P.J. Lefébvre. Springer-Verlag, Berlin.

  11. Jefferson, L.S. and Kimball, S.R. 1989. Insulin regulation of protein synthesis.In Molecular and Cellular Biology of Diabetes Mellitus. Vol. 2, Insulin Action. pp. 133–142. Edited by B. Draznin, S. Melmed and D. LeRoith, Alan R. Liss, Inc., New York.

  12. Jennrich, R. and Sampson, P. 1983. General mixed model analysis of variance.In BMDP Statistical Software. pp. 413–426. Edited by W.J. Dixon. University of California Press, Berkeley.

  13. King, J.L. and Kahn, C.R. 1981. Nonparallel evolution of metabolic and growth-promoting functions of insulin. Nature, Lond. 292: 644–646.

  14. Ludwig, B., Higgs, D.A., Fagerlund, U.H.M. and McBride, J.R. 1977. A preliminary study of insulin participation in the growth regulation of coho salmon (O. kisutch). Can. J. Zool. 55: 1756–1758.

  15. Mommsen, T.P. and Moon, T.W. 1989. Metabolic actions of glucagon-family hormones in liver. Fish Physiol. Biochem. 7: 279–288.

  16. Mommsen, T.P. and Plisetskaya, E.M. 1991. Insulin in fishes and agnathans: History, structure and metabolic regulation. Crit. Rev. Aquat. Sci. 4: (In press).

  17. Morley, J.E. 1987. Neuropeptide regulation of appetite and weight. End. Rev. 8: 256–287.

  18. Murat, J.-C., Plisetskaya, E.M. and Woo, N.Y.S. 1981. Endocrine control of nutrition in cyclostomes and fish. Comp. Biochem. Physiol. 68A: 149–158.

  19. Oshima, I., Hiroto, M., Ohboshi, C. and Shima, K. 1988. Comparison of half-disappearance times, distribution volumes and metabolic clearance rates of exogenous glucagon-like peptide I and glucagon in rats. Regul. Pept. 21: 85–93.

  20. Plisetskaya, E.M. 1990. Glucagon and related peptides (An overview).In Progress in Comparative Endocrinology. pp. 67–72. Edited by A. Epple, C.G. Scanes, M.H. Stetson. Wiley-Liss, New York.

  21. Plisetskaya, E.M., Bhattacharia, S., Dickhoff, W.W. and Gorbman, A. 1984. The effect of insulin on amino acid metabolism and glycogen content in isolated liver cells of juvenile coho salmon,Oncorhynchus kisutch. Comp. Biochem. Physiol. 78A: 773–778.

  22. Plisetskaya, E.M., Dickhoff, W.W., Paquette, T.L. and Gorbman, A. 1986a. The assay of salmon insulin by homologous radioimmunoassay. Fish Physiol. Biochem. 1: 37–43.

  23. Plisetskaya, E.M., Ottolenghi, T., Sheridan, M.A., Mommsen, T. and Gorbman, A. 1989. Metabolic effects of salmon glucagon and glucagon-like peptide in coho and chinook salmon. Gen. Comp. Endocrinol. 73: 205–216.

  24. Plisetskaya, E.M., Pollock, H.G., Rouse, J.B., Hamilton, J.W., Kimmel, J.R. and Gorbman, A. 1985. Characterization of coho salmon (Oncorhynchus kisutch) insulin. Regul. Pept. 11: 105–116.

  25. Plisetskaya, E.M., Pollock, H.G., Rouse, J.B., Hamilton, J.W., Kimmel, J.R. and Gorbman, A. 1986b. Isolation and structures of coho salmon (Oncorhynchus kisutch) glucagon and glucagon-like peptide. Regul. Pept. 14: 57–67.

  26. Plisetskaya, E.M., Pollock, H.G., Rouse, J.B., Hamilton, J.W., Kimmel, J.R., Andrews, P.C. and Gorbman, A. 1986c. Characterization of coho salmon (Oncorhynchus kisutch) islet somatostatins. Gen. Comp. Endocrinol. 63: 252–263.

  27. Plisetskaya, E.M., Sundby, A. and Gorbman, A. 1987. Unorthodox interrelations among pancreatic islet hormones in fish. Am. Zool. 27: 97A (Abstract).

  28. Plisetskaya, E.M. and Sullivan, C.V. 1989. Pancreatic and thyroid hormones in rainbow trout (Salmo gairdneri): What concentration does the liver see? Gen. Comp. Endocrinol. 75: 310–315.

  29. Plisetskaya, E.M., Swanson, P., Bernard, M. and Dickhoff, W.W. (1988). Insulin in coho salmon (Oncorhynchus kisutch) during smoltification. Aquaculture 72: 151–164.

  30. Rao, R.H. and Spathis, G.S. (1987). Intramuscular glucagon as a provocative stimulus for the assessment of pituitary function: growth hormone and cortisol responses. Metabolism 36: 658–663.

  31. Refstie, T. 1991. Applications of breeding schemes. Aquaculture (In press).

  32. Suarez, R.K. and Mommsen, T.P. 1987. Gluconeogenesis in teleost fishes. Can. J. Zool. 65: 1869–1882.

  33. Sundby, A. 1989. Gastrointestinal hormones and growth in fish.In Satellite Symposium on Applications of Comparative Endocrinology to Fish Culture. pp. 113–122. Edited by M. Carrillo, S. Zanuy and J. Planas. Publicacions Universitat de Barcelona, Barcelona.

  34. Tager, H.S., Hohenboken, M. and Markese, 1977. High titer glucagon antisera. Endocrinology 100: 367–372.

  35. Zanuy, S., Carrillo, M., Perez, J., Gutiérrez, J. and Planas, J. 1989. Environmental and nutritional influences on hormonal profiles in cultivated sea bass (Dicentrarchus labrax L.).In Satellite Symposium on Applications of Comparative Endocrinology to Fish Culture, pp. 123–134. Edited by M. Carrillo, S. Zanuy and J. Planas. Publicacions Universitat de Barcelona, Barcelona.

  36. Zapf, J., Froesch, E.R. and Humbel, R.E. 1981. The insulin-like growth factors (IGF) of human serum: Chemical and biological characterization and aspects of their possible physiological role.In Current Topics in Cellular Regulation. Vol. 19: 257–309. Edited by B.L. Horecker and E.R. Stadtman. Academic Press, New York.

Download references

Author information

Additional information

Reported in part at Satellite Symposium on Applications of Comparative Endocrinology to Fish Culture, Almunecar, Spain (Sundby, 1989).

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sundby, A., Eliassen, K., Refstie, T. et al. Plasma levels of insulin, glucagon and glucagon-like peptide in salmonids of different weights. Fish Physiol Biochem 9, 223–230 (1991). https://doi.org/10.1007/BF02265143

Download citation

Keywords

  • salmonids
  • body weight
  • radioimmunoassays
  • insulin
  • glucagon
  • glucagon-like peptide