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Delayed gametogenesis and spawning of sea bass (Dicentrarchus labrax L.) kept under different photoperiod and temperature regimes

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The present work investigates the importance of day length and temperature in the control of reproduction of sea bass, as well as the effectiveness of LHRHa and HCG in inducing spawning out of season in this species. A controlled regime was produced and seasonal cycles with high components of temperature and photoperiod were extended from the summer solstice for at least 6 months, followed by a short photoperiod regime for 3 months before a new increase in these components. Natural spawning in the control fish occurred more frequently in mid February, although it was also observed in January and early March. Temperature manipulation delayed the spawning one month with respect to the controls, although some of the animals entered into gonadal regression. Photoperiodic manipulation delayed maturation for three months with respect to controls but it was necessary to perform hormonal induction of spawning. Although LHRHa and HCG were both applied, only intraperitoneal injections of LHRHa were effective in inducing spawning of sea bass out of season when the temperatures were 17°C. Dephasing between the annual changes in photoperiod in relation to the coordination of the different events of the sexual cycle of sea bass is considered.

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References cited

  1. Allison, L.N. 1951. Delay of spawning of eastern brook trout by means of artificially prolonged light intervals. Progr. Fish. Cult. 13: 111–116.

  2. Barnabé, G. and Paris, J. 1984. Ponte avancée et ponte normale du loupDicentrarchus labrax (L.) a la station de Biologie marine et lagunaire de Sète.In L'Aquaculture du Bar et des Sparidés, pp. 63–72. INRA Publ., Paris.

  3. Batschelet, E. 1981. Circular Statistics in Biology. Academic Press, London.

  4. Billard, R. and Breton, B. 1977. Sensibilité à la température des différentes étapes de la reproduction chez la truite Arc-enciel. Cah. Lab. Montereau. 5: 5–24.

  5. Billard, R. and Breton, B. 1978. Rhythms of reproduction in teleost fish.In Rhythmic activity of fishes. pp. 31–53. Edited by J.E. Thorpe. Academic Press, London.

  6. Billard, R., Breton, E., Fostier, A., Jalabert, B. and Weil, C. 1978. Endocrine control of the teleost reproductive cycle and its relation to external factors: salmonid and cyprinid models.In Comparative Endocrinology. pp. 37–48. Edited by P. Gaillard and H.H. Boer. Elsevier, North Holland Biomedical Press, Amsterdam.

  7. Breton, B., Maisse, G. and Lemenn, E. 1983. Controle photopériodique de la saison de reproduction en salmoniculture: une expérience pilote en Bretagne. Bull. Fr. Piscic. 228: 35–45.

  8. Bromage, N., Whitehead, C., Elliot, J., Breton, B. and Matty, A. 1982. Investigations into the importance of daylength on the photoperiodic control of reproduction in the female rainbow trout.In Proc. Int. Symp. Reprod. Physiol. Fish. pp. 233–236. Compile by C.J.J. Richter and H.J.Th. Goos. Pudoc, Wageningen.

  9. Bromage, N.R., Elliott, J.A.K., Springate, J.R.C. and Whitehead, C. 1984. The effects of constant photoperiods on the timing of spawning in the rainbow trout. Aquaculture 43: 213–223.

  10. Burlier, A. and Billard, R. 1984. Delay of gametogenesis and spawning by constant illumination of rainbow trout (Salmo gairdneri) during the first reproductive cycle. Can. J. Zool. 62: 2183–2187.

  11. Bye, V.J. 1984. The role of environmental factors in the timing of reproductive cycles.In Fish Reproduction: Strategies and Tactics. pp. 187–205. Edited by G.W. Potts and R.J. Wootton. Academic Press, London.

  12. Chauduri, H. 1976. Use of hormones in induced spawning carps. J. Fish. Res. Board Can. 33: 940–947.

  13. Colombo, L., Belvédère, P.C., Marconato, A. and Bentivegna, F. 1982. Pheromones in Teleost Fish.In Proc. Int. Symp. Reprod. Physiol. Fish. pp. 84–94. Compiled by C.J.J. Richter and H.J.Th. Goos, Pudoc, Wageningen.

  14. Combs, B.D., Burrows, R.E. and Bigej, R.G. 1959. The effect of controlled light on the maturation of adult blueback salmon. Progr. Fish Cult. 21: 63–69.

  15. Cook, A.F. and Peter, R.F. 1980. The effect of temperature on the clearance of intraperitoneally-injected gonadotropin in the goldfishCarassius auratus. Aquaculture. 19: 275–286.

  16. De Vlaming, V.L. 1972. Environmental control of teleost reproductive cycles: a brief review. J. Fish Biol. 4: 131–140.

  17. De Vlaming, V.L. 1974. Environmental and endocrine control of teleost reproduction.In Control of sex in fishes. pp. 13–83. Edited by C.B. Schreck. Extension Division, Virginia Polytechnic Institute and State University Blacksburg, Virginia 24061, U.S.A.

  18. Devauchelle, N. 1984. Reproduction décalée du bar (Dicentrarchus labrax) et de la dourade (Sparus aurata).In L'Aquaculture du Bar et des Sparidés. pp. 53–61. INRA. Publ., Paris.

  19. Donaldson, E.M. 1975. Physiological and physiochemical factors associated with maturation and spawning. EIFAC Tech. Pap. EIFAC/T25, 53–71.

  20. Duston, J. and Bromage, N. 1986. Photoperiodic mechanisms and rhythms of reproduction in the female rainbow trout. Fish Physiol. Biochem. (in press).

  21. Fostier, A., Jalabert, B. 1982. Physiological basis of practical means to induce ovulation in fish.In Proc. Int. Symp. Reprod. Physiol. Fish. pp. 164–173. Compiled by C.J.J. Richter and H.J.Th. Goos. Pudoc, Wageningen.

  22. Hazard, T.P. and Eddy, R.E. 1951. Modification of the sexual cycle in brook trout (Salvelinus fontinalis) by control of light. Trans. Am. Fish. Soc. 80: 158–162.

  23. Hontela, A. and Peter, R.E. 1978. Daily cycles in serum gonadotropin levels in the goldfish:Carassius auratus, effects of photoperiod, temperature, and sexual condition, Can. J. Zool 56: 2430–2442.

  24. Htun-Han, M. 1977. The effects of photoperiod on reproduction in fishes: an annotated bibliography. Library Inf. Leaflet, MAFF,Lowestoft, 6: 1–30.

  25. Jalabert, B. and Fostier, A. 1984a. The follicular sensitivityin vitro to maturation inducing hormones in rainbow trout,Salmo gairdneri role of oestradiol-17β. Aquaculture 43: 1–11.

  26. Lalabert, B. and Fostier, A. 1984b. The modulatory effectin vitro of oestradiol-17β, testosterone or cortisol on the output of 17α-hydroxy-20β-dihydroprogesterone by rainbow trout (Salmo gairdneri) ovarian follicles stimulated by the maturational gonadotropin s-GtH. Reprod. Nutr. Dévelop. 24: 127–136.

  27. Lam, T.J. 1982. Applications of endocrinology to fish culture. Can. J. Fish. Aquat. Sci. 39: 111–137.

  28. Lundquist, H. 1980. Influence of photoperiod on growth in Baltic salmon par (Salmo salar L.) with special reference to the effect of precocious sexual maturation. Can. J. Zool. 58: 940–944.

  29. Manning, N.J. and Kime, D.E. 1985. The effect of temperature on testicular steroid production in the rainbow trout,Salmo gairdneri, in vitro. Gen. Comp. Endocrinol. 57: 382.

  30. Peter, R.E. and Hontela, A. 1978. Annual gonadal cycles in teleosts: environmental factors and gonadotropin levels in blood.In Environmental Endocrinology. pp. 20–5. Edited by I. Assenmacher and D.S. Farner. Springer-Verlag, Berlin.

  31. Peter, R.E. and Crim, L.W. 1979. Reproductive endocrinology of fishes: Gonadal cycles and gonadotropin in teleosts. Ann. Rev. Physiol. 41: 323–335.

  32. Prat, F., Carrillo, M., Zanuy, S. and Fostier, A. 1975. Fluctuations of plasma levels of testosterone and 11-ketostestosterone in males and 17β-estradiol in females of sea bass (Dicentrarchus labrax L.) along the year.In 7th Conference of European Society for Comparative Physiology and Biochemistry: Fish Culture (Abstracts). PPU, Barcelona.

  33. Poston, H.A. 1978. Neuroendocrine mediation of photoperiod and other environmental influences on physiological responses in salmonids: a review. Tech. Pap. U.S. Fish Wildl. Serv. 96: 1–14.

  34. Scott, D.B.C. 1979. Environmental timing and the control of reproduction in teleost fish. fish. Symp. Zool. Soc., Lond. 44: 105–132.

  35. Scott, A.P. and Sumpter, J.P. 1983. The control of trout reproduction: basic and applied research on hormones.In Control Processes in Fish Physiology. pp. 176–199. Croom Helm, London and Canberra.

  36. Scott, A.P., Baynes, S.M., Skarphédinsson, O. and Bye, V.J. 1984. Control of spawning time in rainbow trout,Salmo gairdneri, using constant long daylength. Aquaculture. 43: 225–233.

  37. Shehadeh, Z.H., Kö, C.M. and Milisen, K.K. 1973. Validation of anin vivo method for monitoring ovarian development in the grey mullet (Mugil cephalus L.) J. Fish Biol. 5: 479–487.

  38. Shiraishi, Y. and Fukuda, Y. 1966. The relation between the day length and the maturation in four species of salmonid fish. Bull. Freshwater Fish. Res. Lab. 16: 103–111.

  39. Sokolowska, M., Peter, R.E., Nahorniak, C.S. and Chang, J.P. 1985. Seasonal effects of pimozide and des Gly D-Ala) LHRH ethylamide on gonadotropin secretion in goldfish Gen. Comp. Endocrinol. 57: 472–479.

  40. Stacey, N.E. 1984. Control of the timing of ovulation by exogenous and endogenous factors.In Fish Reproduction: Strategies and Tactics. pp. 207–222. Edited by G.W. Potts and R.J. Wootton. Academic Press, London.

  41. Takashima, F. and Yamada, Y. 1984. Control of Maturation in masu salmon by manipulation of photoperiod. Aquaculture 43: 243–257.

  42. Zanuy S. and Carrillo, M. 1984. La salinité: un moyen pour retarder la ponte du barIn L'Aquaculture du Bar et des Sparidés. pp. 73–80. INRA Publ., Paris.

  43. Zohar, Y., Billard, R. and Weil, C. 1984. La reproduction de la daurode (Sparus aurata) et du bar (Dicentrarchus labrax): Connaissance du cycle sexuel et contrôle de la gamétogenèse et de la ponte.In L'Aquaculture du Bar et des Sparidés. pp. 3–24, Edited by G. Barnabé and R. Billard. INRA Publ., Paris.

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Correspondence to Dr. M. Carrillo.

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Zanuy, S., Carrillo, M. & Ruiz, F. Delayed gametogenesis and spawning of sea bass (Dicentrarchus labrax L.) kept under different photoperiod and temperature regimes. Fish Physiol Biochem 2, 53–63 (1986).

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  • daylength
  • temperature
  • LHRHa
  • reproduction
  • delayed spawning
  • intraovarian oocytes
  • maturity index
  • sea bass