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Central regulation of reproduction in teleosts

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As in other vertebrates, reproduction in teleosts depends upon interactions taking place along the brain-pituitary-gonads axis. At the central level, these interactions involve at least three types of factors:

A gonadotrophin-releasing factor which has recently been isolated from chum salmon brain extracts. This decapeptide, whose structure is (Trp7-Leu8)-LHRH, appears to have a widespread distribution among teleosts, and is less active that LHRH or LHRH analogues in releasing gonadotrophin from the teleost pituitary. Immunohistochemical and quantitative studies have demonstrated that Gn-RH neurons are mainly located in the ventral telencephalon and the preoptic area, while projections are found in the entire brain and the pituitary gland.

A gonadotrophin release-inhibiting factor has been demonstrated in the anterior preoptic region of the goldfish and a large set of data suggests that dopamine has GRIF activity in goldfish, and in other teleost species, by direct action on the gonadotrophs. Accordingly, a dopaminergic preoptico-hypophyseal pathway could be demonstrated in the goldfish brain.

Sex steroids exert, depending on the dosages, either a negative feedback in sexually mature fish or a positive feedback in immature fish. Such a positive feedback is caused by estrogens and aromatizable androgens. Accordingly, the brain of teleosts contains high levels of aromatase activity in particular in the telencephalon and anterior hypothalamus. The distribution of estrogens concentrating cells within the brain is consistent with possible interactions with Gn-RH or catecholaminergic neurons at the level of certain brain territories.

These data are discussed in relation with the functional significance of different brain areas where interactions between these different factors possibly take place, in particular the terminal nerve, the ventral telencephalon, the preoptic area and nucleus lateralis tuberis.

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References cited

  1. Barnett, F.H., Sohn, J., Reichlin, S. and Jackson, I.M.D. 1982. Three luteinizing hormone-releasing hormone like sustances in a teleost fish brain: None identical with the mammalian LH-RH decapeptide. Biochem. Biophys. Res. Commun. 105: 209–216.

  2. Billard, R., Alagarswami, K., Peter, R.E. and Breton, B. 1983. Potentialisation par le pimozide des effets du LHRH-A sur la sécrétion gonadotrope hypophysaire, l'ovulation et la spermiation chez la Carpe commune (Cyprinus carpio). C. R. Acad. Sci., Paris 296: 181–184.

  3. Borg, B., Goos, H.J.Th., Terlou, M. 1982. LHRH-immunoreactive cells in the brain of the threespined stickleback,Gasterosteus aculeatus. Cell. Tiss. Res. 226: 695–699.

  4. Breton, B., Weil, C. and Jalabert, B. 1972. Activité réciproque des facteurs hypothalamiques de Bélier et de poissons téléostéens sur la sécrétionin vitro des hormones gonadotropes c-HG et o-LH respectivement par des hypophyses de Carpe et de Bélier. C. R. Acad. Sci., Paris 274: 2530–2533.

  5. Breton, B., Motin, A., Kah, O., Le Menn, F., Geoffre, S., Précigoux, G. and Chambolle, P. 1984. Dosage radioimmunoligique homologue d'un facteur hypothalamique de stimulation de la fonction gonadotrope hypophysaire de Saumon s-Gn-RH. C. R. Acad. Sci., Paris 299: 383–388.

  6. Breton, B., Motin, A., Billard, R., Kah, O., Geoffre, S. and Précigoux, G. 1986. Development of a salmon Gn-RH radioimmunoassay and its application to the determination of the Gn-RH content in the brain and the pituitary gland during the periovulatory period in the brown trout (Salmo trutta L.). Relationships with the plasma and pituitary gonadotropin (GtH). Gen. Comp. Endocrinol. 61: 109–119.

  7. Callard, G.V. 1983. Androgen and estrogen actions in the vertebrate brain. Am. Zool. 23: 607–620.

  8. Chang, J.P. and Peter, R.E. 1983a. Effects of dopamine on gonadotropin release in female goldfish,Carassius auratus. Neuroendocrinology 36: 351–357.

  9. Chang, J.P. and Peter, R.E. 1983b. Effects of pimozide and Des Gly10,(D-Ala6) Luteinizing hormone-releasing hormone ethylamide on serum gonadotropin concentrations, germinal vesicle migration, and ovulation in female goldfish,Carassius auratus. Gen. Comp. Endocrinol. 52: 30–37.

  10. Chang, J.P., Cook, A.F. and Peter, R.E. 1983. Influence of catecholamines on gonadotropin secretion in goldfish,Carassius auratus. Gen. Comp. Endocrinol. 49: 22–31.

  11. Chang, J.P., MacKenzie, D.S., Goud, D.R. and Peter, R.E. 1984a. Effects of dopamine and norepinephrine on in vitro spontaneous and gonadotropin-releasing hormone-induced gonadotropin release by dispersed cells or fragments of the goldfish pituitary. Life Sci. 35: 2027–2033.

  12. Chang, J.P., Peter, R.E. and Crim, L.W. 1984b. Effects of dopamine and apomorphine on gonadotropin release from the transplanted pars distalis in goldfish. Gen. Comp. Endocrinol. 55: 347–350.

  13. Chang, J.P., Peter, R.E., Nahorniak, C.S. and Sokolowska, M. 1984c. Effects of catecholaminergic agonists and antagonists on serum gonadotropin concentrations and ovulation in goldfish: evidence for specificity of dopamine inhibition of gonadotropin secretion. Gen. Comp. Endocrinol. 55: 351–360.

  14. Crim, L.W., Peter, R.E. and Billard, R. 1981. Onset of gonadotropin hormone accumulation in the immature trout pituitary gland in response to estrogen and aromatizable androgen steroid hormones. Gen. Comp. Endocrinol. 44: 374–381.

  15. Demski, L.S. 1984. The evolution of neuroanatomical substrates of reproductive behavior: sex steroid and LH-RH-specific pathways including the terminal nerve. Am. Zool. 24: 809–830.

  16. Demski, L.S. and Dulka, J.G. 1984. Functional-anatomical studies on sperm release evoked by eletrical stimulation of the olfactory tract in goldfish. Brain Res. 291: 241–247.

  17. Demski, L.S. and Northcutt, R.G. 1983. The terminal nerve: A new chemosensory system in vertebrates? Science. 220: 435–437.

  18. Doving, K.B. and Selset, R. 1980. Behavior patterns in cod released by electrical stimulation of olfactory tract bundlets. Science 207: 559–560.

  19. Dufour, S. 1985. La fonction gonadotrope de l'anguille européenne,Anguilla anguilla L., au stade argenté (au moment du départ pour la migration de reproduction): les mécanismes de son blocage et sa stimulation expérimentale. Thèse Dr. Sci., Univ. Paris VI.

  20. Dufour, S., Pasqualini, C., Kerdelhué, B. and Fontaine, Y.A. 1982. Presence and distribution of radioimmunoassayable LH-RH in the European eel (Anguilla anguilla). Neuropeptide 3: 159–171.

  21. Dufour, S., Delerue-Le Belle, N. and Fontaine, Y.A. 1984. Stimulation de la libération d'hormone gonadotrope et du développement des gonades sous l'effet conjugué du pimozide et d'un agoniste de la LH-RH chez l'Anguille argentée prétraitée à l'oestradiol. C. R. Acad. Sci., Paris 299: 231–234.

  22. Ekengren, B. 1975. Aminergic nuclei in the hypothalamus of the roachLeuciscus rutilus. Cell Tiss. Res. 159: 493–502.

  23. Goos, H.J.Th. and Murathanoglu, O. 1977. Localisation of gonadotrophin releasing hormone (GRH) in the forebrain and neurohypohysis of the trout (Salmo gairdneri). Cell Tiss. Res. 181: 163–168.

  24. Goos, H.J.Th., de Leeuw, R., de Zoeten-Kamp, C., Peute, J. and Blähser, S. 1985. Gonadotropin-releasing hormone-immunoreactive neuronal structures in the brain and pituitary of the African catfish,Clarias gariepinus (Burchell). Cell Tiss. Res. 241: 593–596.

  25. Halpern-Sebold, L.R., and Schreibman, M.P. 1983. Ontogeny of centers containing luteinizing hormone-releasing hormone in the brain of platyfish (Xiphophorus maculatus) as determined by immunocytochemistry. Cell Tiss. Res. 229: 75–84.

  26. Hontela, A. and Peter, R.E. 1980. Effects of pinealectomy, blinding, and sexual condition on serum gonadotropin levels in the goldfish. Gen. Comp. Endocrinol. 40: 168–179.

  27. Idler, D.R. and Crim, L.W. 1982. Gonadotropin releasing factor(s) (GtH-RF) from hypothalamus of winter flounder.In: Proceedings of the ninth international symposium on Comparative Endocrinology. Edited by B. Lofts, University of Hong-Kong Press, Hong-Kong (In press).

  28. Jones, S.W., Adams, P.R., Brownstein, M.J. and Rivier J.E. 1984. Teleost luteinizing hormone-releasing hormone action on bullfrog sympathetic ganglia is consistent with role as neurotransmitter. Neurosci. 4: 420–429.

  29. Kah, O., Breton, B., Dulka, J.G., Nunez-Rodriguez, J., Peter, R.E., Corrigan, A., Rivier, J.E. and Vale, W.W. (1986a) A reinvestigation of the Gn-RH (gonadotrophin-releasing hormone) systems in the goldfish brain using antibodies to salmon Gn-RH. Cell. Tiss. Res. In press.

  30. Kah, O., Chambolle, P., Dubourg, P. and Dubois, M.P. 1984a. Immunocytochemical localization of luteinizing hormone-releasing hormone in the brain of the goldfishCarassius auratus. Gen. Comp. Endocrinol. 53: 107–115.

  31. Kah, O., Chambolle, P., Thibault, J. and Geffard, M. 1984b. Existence of dopaminergic neurons in the preoptic region of the goldfish. Neurosci. Lett. 48: 293–298.

  32. Kah, O., Dubourg, P., Chambolle, P. and Calas, A. 1984c. Ultrastructural identification of catecholaminergic fibers in the goldfish pituitary. Cell. Tiss. Res. 238: 621–626.

  33. Kah, O., Dubourg, P., Onteniente, B., Geffard, M. and Calas, A. 1986b. The dopaminergic innervation of the goldfish pituitary gland: An electron microscope immunocytochemical study using antibodies to dopamine. Cell Tissue Res. In press.

  34. Kim, Y.S., Stumpf, W.E. and Sar, M. 1978. Topography of estrogen target cells in the forebrain of goldfish,Carassius auratus. J. Comp. Neurol. 182: 611–620.

  35. King, J.A. and Millar, R.P. 1980. Comparative aspects of luteinizing hormone-releasing hormone structure and function in vertebrate phylogeny. Endocrinol. 106: 707–717.

  36. King, J.A. and Millar, R.P. 1982. Structure of chicken hypothalamic luteinizing hormone releasing hormone. II. Isolation and characterization. J. Biol. Chem. 257: 10729–10732.

  37. Koyama, Y., Satou, M., Oka, Y. and Ueda, K. 1984. Involvement of the telencephalic hemispheres and the preoptic area in sexual behavior of the male goldfish,Carassius auratus: a brain-lesion study. Behav. Neur. Biol. 40: 70–86.

  38. Kyle, A.L. and Peter, R.E. 1982. Effects of forebrain lesions on spawning behavior in the male goldfish. Physiol. Behav. 28: 1103–1109.

  39. Kyle, A.L., Stell, W.K. and Kah, O. 1985a. Luteinizing hormone-releasing hormone (LHRH)-immunoreactive (ir) cells of the terminal nerve, but not other LHRH-ir neurons in the goldfish brain, are also FMRFamide-and tachykinin-ir. Annual Meeting of the Society for Neuroscience, Dallas, Abstr. Vol. 1: 107–108.

  40. Kyle, A.L., Stacey, N.E., Peter, R.E. and Billard, R. 1985b. Elevations in gonadotrophin concentrations and milt volumes as a result of spawning behavior in the goldfish. Gen. Comp. Endocrinol. 57: 10–22.

  41. De Leeuw, R., Resink, J.W., Rooyakkers, E.J.M. and Goos, H.J.Th. 1985. Pimozide modulates the luteinizing hormone-releasing hormone effect on gonadotropin release in the african catfish,Clarias lazera. Gen. Comp. Endocrinol. 58: 120–127.

  42. Miyamoto, K., Hasegawa, Y., Minegishi, T., Nomura, M., Takahashi, Y., Igarashi, M., Kangawa, K. and Matsuo, H. 1982. Isolation and characterization of chicken hypothalamic luteinizing hormone-releasing hormone. Biochem. Biophys. Res. Commun. 107: 820–827.

  43. Miyamoto, K., Hasegawa, Y., Nomura, M., Igarashi, M., Kangawa, K. and Matsuo, H. 1984. Identification of the second gonadotropin-releasing hormone in the chiken hypothalamus: evidence that gonadotropin secretion is probably controlled by two distinct gonadotropinreleasing hormone in avian species. Proc. Natl. Acad. Aci. USA, 81: 3874–3878.

  44. Merchenthaler, I., Göres, T., Sétalo, G., Petrusz, P. and Llerko, B. 1984. Gonadotropin-releasing hormone (G-RH) neurons and pathways in the rat brain. Cell. Tiss. Res. 237: 15–29.

  45. Münz, B., Stumpf, W.E. and Jennes, L. 1981. LHRH systems in the brain of platyfish. Brain Res. 221: 1–13.

  46. Münz, H., Claas, B, Stumpf, W.E. and Jennes, L. 1982. Centrifugal innervation of the retina by luteinizing hormone-releasing hormone (LHRH) immunoreactive telencephalic neurons in teleostean fishes. Cell. Tiss. Res. 222: 313–323.

  47. Nagahama, Y. and Peter, R. E. 1982. Effects of brain lesions on gonadotrop ultrastructure and serum gonadotropin levels in goldfish. Cel. Tiss. Res. 225: 259–265.

  48. Nozaki, M. and Kobayashi, H. 1979. Distribution of LHRH-like substance in the vertebrate brain as revealed by immuno-histochemistry. Arch. Histol. Jap. 42: 201–209.

  49. Olivereau, M. and Callard, G. 1985. Distribution of cell types and aromatase activity in the sculpin (Myoxocephalus) pituitary. Gen. Comp. Endocrinol. 58: 280–290.

  50. Pennipacker, K.R., Fine, M.L. and Stewart, J.K. 1985. Effects of gonadal steroids on catecholamine levels in the brain of the oyster toadfish. Neurosci. Lett. 55: 203–206.

  51. Peter, R.E. 1970. Hypothalamic control of thyroid gland activity and gonadal activity in the goldfish,Carassius auratus. Gen. Comp. Endocrinol. 14: 334–356.

  52. Peter, R.E. 1982. Nature, localization and actions of neurohormones regulating gonadotropin secretion in teleosts.In: Reproductive Physiology of Fish. pp. 30–39. Edited by C.J.J. Richter and H.J.Th. Goos. Pudoc, Wageningen, The Netherlands.

  53. Peter, R.E. 1983. The brain and neurohormones in teleost reproduction.In: Fish Physiology. pp. 97–135. Edited W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press, New-York.

  54. Peter, R.E. and Paulencu, C.R. 1980. Involvement of the preoptic region in gonadotropin release-inhibition in goldfish,Carassius auratus. Neuroendocrinology 31: 133–141.

  55. Peter, R.E., Crim, L.W., Goos, H.J.Th. and Crim, J.W. 1978. Lesioning studies on the gravid female goldfish: neuroendocrine regulation of ovulation. Gen. Comp. Endocrinol. 35: 391–401.

  56. Peter, R.E., Nahorniak, C.S., Chang, J.P. and Crim, L.W. 1984. Gonadotropin release from the pars distalis,Carassius auratus, transplanted beside the brain or into the brain ventricles: additional evidence for gonadotropin-release-inhibitory factor. Gen. Comp. Endocrinol. 55: 337–346.

  57. Peter, R.E., Nahorniak, C.S., Sokolowska, M., Chang, J.P., Rivier, J.E., Vale, W.W., King, J.A. and Millar, R.P. 1985. Structure-activity relationships of mammalian, chicken and salmon gonadotropin releasing hormonesin vivo in goldfish. Gen. Comp. Endocrinol. 58: 231–242.

  58. Sakuma, Y. and Pfaff, D.W. 1980. LHRH in the mesencephalic central gray can potentiate lordosis reflex of female rats. Nature, London. 283: 566–567.

  59. Schreibman, M.P., Pertschuk, L.P., Rainford, E.A., Margolis-Kazan, H. and Gelber, S.G. 1982. The histochemical localization of steroid binding sites in the pituitary gland of a teleost (the platyfish). Cell. Tiss. Res. 226: 523–530.

  60. Schreibman, M.P., Halpern-Sebold, L., Ferin, M., Margolis-Kazan, H. and Goos, H.J.Th. 1983. The effect of hypophysectomy and gonadotropin administration on the distribution and quantity of LH-RH in the brains of platyfish: a combined immunocytochemistry and radioimmunoassay study. Brain Res. 267: 293–300.

  61. Schreibman, M.P., Margolis-Kazan, H., Halpern-Sebold, L., O'Neill, P.A. and Silverman, R.C. 1984. Structural and functional links between olfactory and reproductive systems: puberty-related changes in olfactory epithelium. Brain Res. 302: 180–183.

  62. Sherwood, N., Eiden, L., Brownstein, M., Spiess, J., Rivier, J. and Vale, W. 1983. Characterization of a teleost Gn-RH. Proc. Natl. Acad. Sci. USA 80: 2794–2797.

  63. Sherwood, N.M., Harvey, B., Brownstein, M.J. and Eiden, L.E. 1984. Gonadotropin-releasing hormone (Gn-RH) in striped mullet (Mugil cephalus), milkfish (Chanos chanos), and rainbow trout (Salmo gairdneri): Comparison with salmon Gn-RH. Gen. Comp. Endocrinol. 55: 174–181.

  64. Sokolowska, M., Peter, R.E., Nahorniak, C.S. and Chang, J.P. 1985. Seasonal effects of pimozide and des Gly10 (d-Ala6) LH-RH ethylamide on gonadotropin secretion in goldfish. Gen. Comp. Endocrinol. 57: 472–479.

  65. Springer, A.D. 1983. Centrifugal innervation of goldfish retina from ganglion cells of the nervus terminalis. J. Comp. Neurol. 214: 404–415.

  66. Stacey, N.E., Cook, A.F. and Peter, R.E. 1979. Ovulatory surge of gonadotrophin in the goldfish,Carassius auratus. Gen. Comp. Endocrinol. 37: 246–249.

  67. Stacey, N.E. and Kyle, A.L. 1983. Effects of olfactory tract lesions on sexual and feeding behavior in the goldfish. Physiol. Behav. 30: 621–628.

  68. Stell, W.K., Walker, S.E., Chohan, K.S. and Ball, A.K. 1984. The goldfish nervus terminalis: a luteinizing hormone-releasing hormone and a mollusc cardicexcitatory peptide immunoreactive olfactoretinal pathway. Proc. Natl. Acad. Sci. USA, 81: 940–944.

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Kah, O. Central regulation of reproduction in teleosts. Fish Physiol Biochem 2, 25–34 (1986). https://doi.org/10.1007/BF02264071

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  • neuroendocrine control of reproduction
  • gonadotrophin-releasing hormone
  • dopamine
  • steroid feedback
  • sexual behavior
  • pituitary gland
  • teleost fishes