, Volume 36, Issue 1, pp 167–173 | Cite as

Determination of sulbutiamine and its disulfide derivatives in human plasma by HPLC using on-line post-column reactors and fluorimetric detection

  • P. Gelé
  • C. Boursier-Neyret
  • M. Lesourd
  • C. Sauveur


A new direct HPLC procedure for the simultaneous determination of sulbutiamine (Arcalion) and other thiamine disulfides in human plasma has been developed. The method involves an automated solidphase extraction on octadecylsilyl (C18) cartridges and chromatographic separation of the compounds on an RP Select B column with gradient elution using methanol and phosphate buffer. Detection was by fluorescence of the resulting thiochromes obtained from two on-line post-column reactors. Optimization of post-column reaction parameters has been achieved. This method has been proved to be highly selective for the determination of the thiamine disulfide derivatives and quantitation limits of 5 ng·ml−1 were obtained for each compound in human plasma. Linearity was in the range 5–200 ng·ml−1. Precision and accuracy were also demonstrated by within-day and between-day assays, and showed the good reliability of the method.

Key Words

Column liquid chromatography Solid-phase extraction On-line post-column reaction Sulbutiamine Thiamine disulfide 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • P. Gelé
    • 1
  • C. Boursier-Neyret
    • 1
  • M. Lesourd
    • 2
  • C. Sauveur
    • 1
  1. 1.Department of Bio-AnalysisBio-Pharmacie ServierOrléansFrance
  2. 2.Institut de Recherches Internationales ServierCourbevoie CédexFrance

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