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Analysis of catechins and proanthocyanidins in grape seeds by HPLC with photodiode array detection

Summary

Reversed-phase, high-performance liquid chromatography coupled to photodiode array detection has been used to analyse catechins and proanthocyanidins extracted from grape seeds. Results show that the ethyl acetate fraction obtained by passing extracts of samples adjusted to pH 7.0 through preconditioned C18 SEP-PAK cartridges contains several catechins and proanthocyanidins. Seven peaks have been assigned to standard catechins and proanthocyanidins on the basis of their retention times and UV spectra. Other peaks which appear in the chromatogram show spectral behaviour similar to that of standard catechins and proanthocyanidins.

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Revilla, E., Bourzeix, M. & Alonso, E. Analysis of catechins and proanthocyanidins in grape seeds by HPLC with photodiode array detection. Chromatographia 31, 465–468 (1991). https://doi.org/10.1007/BF02262390

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Key Words

  • Column liquid chromatography
  • Catechins
  • Grape seeds
  • Proanthocyanidins
  • UV-visible spectroscopy/diode array detection