Chromosome Research

, Volume 4, Issue 7, pp 500–506

Molecular features of theTSPY gene of gibbons and Old World monkeys

Article

Abstract

Restriction pattern, chromosome localization and the sequence of the testis-specific gene,TSPY, were investigated in the white-handed gibbon, agile gibbon, siamang, hamadryas baboon and Japanese monkey. Southern blot analysis showed theTSPY gene to be male specific in the primates used and disclosed variability of restriction pattern in gibbons. Fluorescencein situ hybridization demonstrated that the probe ppTSPY2372, biotinylated using polymerase chain reaction, is located as a slight signal in the proximal long arm of the Y chromosome of the white-handed gibbon, hamadryas baboon and Japanese monkey and in the middle long arm of the Y chromosome of the siamang, while a faint signal and an intense signal were detected in the proximal long arm of the Y chromosome of the agile gibbon. These findings allow us to speculate that the gibbons might have evolved some structural differentiation in theTSPY gene. The first introns of theTSPY genes were sequenced and compared. One hundred thirty-seven of 606 sites were found to be variable, and 10 deletions/insertions were noted among these gibbons, two species of Old World monkeys and human. Sequence similarity ranged from 81.7% between humans and hamadryas baboons to 98.7% between Japanese monkeys and hamadryas baboons. These sequences may be of great use in future studies for resolving the phylogeny of gibbons and Old World monkeys.

Key words

gibbons Old World monkeys TSPY gene Y chromosome 

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Copyright information

© Rapid Science Publishers 1996

Authors and Affiliations

  1. 1.Department of Cellular and Molecular Biology, Primate Research InstituteKyoto UniversityInuyama, AichiJapan
  2. 2.Department of Evolution and Phylogeny, Primate Research InstituteKyoto UniversityInuyama, AichiJapan

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