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European Journal of Nuclear Medicine

, Volume 20, Issue 1, pp 20–25 | Cite as

Effect of mitochondrial viability and metabolism on technetium-99m-sestamibi myocardial retention

  • Paul Crane
  • Robert Laliberté
  • Stuart Heminway
  • Martin Thoolen
  • Cesare Orlandi
Original Article

Abstract

This study investigated the mechanism of myocardial retention of technetium-99m-sestamibi.99mTc-sestamibi was injected intravenously into guinea pigs, and the myocardium was homogenized and fractionated by differential centrifugation. More than 90% of myocardial99mTc-sestamibi was localized within the mitochondrial fraction. Calcium was found to release99mTc-sestamibi from the mitochondrial fraction, with an IC50 of 2.54±0.98 mM. This effect was potentiated by NaCl, and inhibited by the mitochondrial calcium channel blocker ruthenium red. In vitro uptake of99mTc-sestamibi was found to increase from 10.5% ± 3.0% to 61.2% ± 0.2% with the addition of 10 mM succinate, indicating that respiration is involved. Since irreversible ischemia results in cellular and mitochondrial calcium “overload” and loss of mitochondrial metabolic function,99mTc-sestamibi should not be retained in necrotic or irreversibly ischemic myocardium, and could potentially act as a sensitive indicator of myocardial cell viability.

Key words

Technetium-99m-sestamibi Myocardial viability Calcium Ischemia 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Paul Crane
    • 1
  • Robert Laliberté
    • 1
  • Stuart Heminway
    • 1
  • Martin Thoolen
    • 1
  • Cesare Orlandi
    • 1
  1. 1.Radiopharmaceutical DivisionDu Pont Merck Pharmaceutical CompanyNo. BillericaUSA

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