An LCEC method for the analysis of synthetic amino acids in fruit juices
- 20 Downloads
A fast and simple HPLC-method for the determination of synthetic amino acids in adulterated orange juice has been developed. The amino acid enantiomers were derivatized with a chiral reagent and the derivatives separated on a 3 μm particle size C18 column. An electrochemical detector operating in the oxidative mode was used for detection. The potential at which the derivatives are oxidized was determined by cyclic voltammetry.
By using selective (electrochemical) detection it is possible to reduce the sample clean-up to simple centrifugation and filtration steps.
Key WordsColumn liquid chromatography Electrochemical detection Amino acid enantiomers OPA-TATG Fruit juices
Unable to display preview. Download preview PDF.
- L. Stryer, “Biochemistry”, W. H. Freeman and Company, New York, 2nd edition 1981, p. 22.Google Scholar
- H. Kasteleyn, W. Ooghe, P. Sandra, I. Temmerman, Journal of High Resolution Chromatography & Chromatography Communications,7, 284 (1984).Google Scholar
- S. Einarsson, S. Folestad, B. Josefsson, Journal of Liquid Chromatography,10, 1589 (1987).Google Scholar
- W. Heineman, P. Kissinger, “Laboratory Techniques in Electroanalytical Chemistry”, Marcel Dekker, Inc., New York and Basel, 1984, p. 86.Google Scholar
- C. K. Lim, “H.p.L.c. of small molecules: a practical approach”, IRL Press Limited, Oxford · Washington DC, 1986, p. 22.Google Scholar