Concentration gradients for monoamine metabolites in lumbar cerebrospinal fluid
Concentration gradients in lumbar cerebrospinal fluid (CSF) for the monoamine metabolites homovanillic acid (HVA), 5-hydroxy-indoleacetic acid (5-HIAA) and 4-hydroxy-3-methoxyphenylglycol (HMPG) were studied in 9 healthy controls and 47 neuropsychiatric patients without diseases causing disturbed CSF circulation. In a serial sampling of the first 24 ml of CSF, steep concentration gradients between the first (0–4 th ml) and last (21th–24th ml) portions of CSF were found for HVA (99±59% increase; p<0.001) and 5-HIAA (88±54% increase; p<0.001), while the concentration gradient was slight for HMPG (11±7% increase; p<0.001). The existence of marked concentration gradients for the monoamine metabolites HVA and 5-HIAA gives further evidence for an active transport system for these metabolites and indicates that the lumbar CSF-HVA and 5-HIAA levels reflect the dopamine and serotonin metabolism in the brain. Moreover, the existence of pronounced concentration gradients for HVA and 5-HIAA levels reflect the dopamine and serotonin metabolism in the brain. Moreover, the existence of pronounced concentration gradients for HVA and 5-HIAA stresses the importance of making analyses on a standardized volume of CSF.
KeywordsHomovanillic acid (HVA) 5-hydroxy-indoleacetic acid (5-HIAA) 4-hydroxy-3-methoxyphenylglycol (HMGP) cerebrospinal fluid (CSF) concentration gradients
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- Blennow K, Wallin A, Gottfries CG, Karlsson I, Månsson JE, Skoog I, Svennerholm L, Wikkelsö C (1991a) Cerebrospinal fluid monoamine metabolites in 114 healthy individuals 18–88 years of age (submitted)Google Scholar
- Blennow K, Rybo E, Wallin A, Gottfries CG, Svennerholm L (1991c) Cerebrospinal fluid cytology in Alzheimer's disease. Dementia 2: 25–29Google Scholar
- Blennow K, Fredman P, Wallin A, Gottfries CG, Långström G, Svennerholm L (1992) Protein analyses in cerebrospinal fluid. I. Influence of concentration gradients for proteins on CSF/S albumin ratio. Eur Neurol (in press)Google Scholar
- Cutler N, Kay A, Ågren H, Linnoila M, Potter W (1988) Cerebrospinal fluid monoamine metabolites levels in 32 healthy men aged 19–77 years. Brain Dysfunct 1: 192–196Google Scholar
- Ebert M, Kartzinel R, Cowdry R, Goodwin F (1980) Cerebrospinal fluid amine metabolites and the probenicide test. In: Wood JH (ed) Neurobiology of cerebrospinal fluid. Plenum Press, New York, pp 97–112Google Scholar
- Kopin IJ, Gordon EK, Jimerson DC, Polinsky RJ (1983) Relation between plasma and cerebrospinal fluid levels of 3-methoxy-4-hydroxyphenylglycol. Science 219: 73–75Google Scholar
- Post RM, Goodwin EK, Gordon E (1973) Amine metabolism in human cerebro spinal fluid: effects of cord transection and spinal fluid block. Science 179: 879–899Google Scholar
- Swahn C-G, Sandgärde B, Wiesel F-A, Sedvall G (1976) Simultaneous deter-mination of the three major monoamine metabolites in brain tissue and body fluids by a mass fragmentographic method. Psychopharmacology 48: 147–152Google Scholar
- Wood JH (1980) Sites of origin and cerebrospinal fluid concentration gradients: neurotransmitters, their precursors and metabolites, and cyclic nucleotides. In: Wood JH (ed) Neurobiology of cerebrospinal fluid. Plenum Press, New York, pp 53–62Google Scholar