, Volume 14, Issue 4, pp 212–215 | Cite as

Determination of antimicrobial drug Ro 12-6995 (a 2,4-diamino-5-benzylpyrimidine) in blood plasma by high-performance liquid chromatography

  • P. Haefelfinger


A HPLC procedure for the determination of Ro 12-6995, a new antimicrobial drug, in blood plasma is described. Adsorption liquid chromatography with LiChrosorb Si 60 as stationary phase with fluorescence detection was used to quantify the drug, which is extracted from plasma with n-butyl acetate. Due to the high fluorescence of Ro 12-6995, the plasma extracts can be injected directly onto the column and no evaporation step in the sample preparation is necessary. The assay is rapid, accurate and sensitive, and easily applicable to the determination of plasma series.

Key Words

Antimicrobial drug Blood plasma HPLC Fluorescence detection 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    R. W. Bury andM. L. Mashford, J. Chromatogr.163, 114 (1979).Google Scholar
  2. [2]
    V. Ascalone, J. HRC & CC5, 261 (1980).Google Scholar
  3. [3]
    T. B. Vree, Y. A. Hekster, A. M. Baars andE. Van der Kleijn, J. Chromatogr.145, 496 (1978).Google Scholar
  4. [4]
    D. Westerlund, J. Carlqvist andA. Theodorsen, Acta Pharm. Suec.16, 187 (1979).Google Scholar
  5. [5]
    N. E. Saris, Z. Klin. Chem. Klin. Biochem.13, 523 (1975).Google Scholar
  6. [6]
    K. Harzer, J. Chromatogr.183, 115 (1980).Google Scholar
  7. [7]
    Rheodyne Technical Notes 1, p. 5 (1979).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1981

Authors and Affiliations

  • P. Haefelfinger
    • 1
  1. 1.Biological Pharmaceutical Research DepartmentF. Hoffmann-La Roche & Co. Ltd.BasleSwitzerland

Personalised recommendations