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Mapping of the porcine urate oxidase and transforming growth factor beta 2 genes by fluorescencein situ hybridization

Abstract

We have mapped two genes from human chromosome 1, urate oxidase (UOX) and transforming growth factor beta 2 (TGFB2), by fluorescencein situ hybridization (FISH) in the pig genome. Porcine-specific polymerase chain reaction (PCR) primers for both genes were designed from the porcine cDNA sequence. With the help of these primers yeast artificial chromosome (YAC) clones forUOX andTGFB2 were isolated from a pig YAC library. These DNA probes were used for FISH analysis.TGFB2 was localized to SSC 10p16. With the YAC probe forUOX two porcine chromosome regions 6q26 and 6q32, revealed specific signals. These results, help to refine the comparative mapping data between human and pig.

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Correspondence to H. Hameister.

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accepted for publication by H. Schmidt

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Rettenberger, G., Leeb, T., Meier-Ewert, S. et al. Mapping of the porcine urate oxidase and transforming growth factor beta 2 genes by fluorescencein situ hybridization. Chromosome Res 4, 147–150 (1996). https://doi.org/10.1007/BF02259708

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Key words

  • fluorescencein situ hybridization
  • gene mapping
  • pig
  • transforming growth
  • factor
  • beta 2
  • urate oxidase