We have mapped two genes from human chromosome 1, urate oxidase (UOX) and transforming growth factor beta 2 (TGFB2), by fluorescencein situ hybridization (FISH) in the pig genome. Porcine-specific polymerase chain reaction (PCR) primers for both genes were designed from the porcine cDNA sequence. With the help of these primers yeast artificial chromosome (YAC) clones forUOX andTGFB2 were isolated from a pig YAC library. These DNA probes were used for FISH analysis.TGFB2 was localized to SSC 10p16. With the YAC probe forUOX two porcine chromosome regions 6q26 and 6q32, revealed specific signals. These results, help to refine the comparative mapping data between human and pig.
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Archibald AL, Haley CS, Brown JF et al. (1995) The PiGMaP consortium linkage map of the pig(Sus srofa) Mammalian Genome 6: 157–175.
Barton DE, Foellmer BE, Du J et al. (1988) Chromosomal mapping of genes for transforming growth factor beta-2 and beta-3 in man and mouse: dispersion of TGF-beta gene family.Oncogene Res 3: 323–331.
Gustavsson G. (1988) Standardized GTG karyotype of the domestic pig.Hereditas 109: 151–177.
Johansson M, Ellegren H, Anderson L (1995) Comparative mapping reveals extensive linkage conservation-but with gene order rearrangements-between the pig and human genomes.Genomics 25: 682–690.
Leeb T, Rettenberger G, Hameister H, Brem G, Brenig B (1995) Construction of a porcine YAC library and mapping of the cardiac muscle ryanodin receptor gene to chromosome 14q22–q23.Mammalian Genome 6: 37–41.
Lichter P, Tang C-J C, Call K et al. (1990) High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.Science 247: 64–69.
O'Brien S (1991). Mammalian genome mapping.Curr Opin Genet Dev 1: 105–111.
O'Brien SJ, Womack JE, Lyons L.A. et al. (1993). Anchored reference loci for comparative genome mapping in mammals.Nature Genet 3: 103–112.
O'Brien SJ, Marshall Graves JA (1991) Report of the Committee on Comparative Gene Mapping.Cytogenet Cell Genet 58: 1124–1151.
Rettenberger G, Burkhardt E, Adham I M et al. (1994) Assignment of the Leydig-insulin like hormone to porcine chromosome 2q12–q13 by somatic cell hybrid analysis and fluorescence in situ hybridization.Mammalian Genome 5: 307–309.
Rettenberger G, Klett C, Zechner U et al. (1995) Visualization of the conservation between pigs and humans by heterologous chromosomal painting.Genomics 26: 372–378.
Rettenberger G, Bruch J, Fries R, Archibald A L, Hameister H (1996) Assignment of 19 porcine type 1 loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig.Mammalian Genome, in press.
Yeldandi AV Patel YD, Liao M et al. (1992) Localization of the human urate oxidase gene (UOX) to 1p22.Cytogenet Cell Genet 61: 121–122.
Yerle M, Lahbib-Mansais Y, Mellink C et al. (1995) The PiG-Map consortium cytogenetic map of the domestic pig (Sus srofa domestica).Mammalian Genome 6: 176–186.
Zehetner G, Lehrach H (1994) The reference library system—sharing biological material and experimental data.Nature 367: 489–491.
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Rettenberger, G., Leeb, T., Meier-Ewert, S. et al. Mapping of the porcine urate oxidase and transforming growth factor beta 2 genes by fluorescencein situ hybridization. Chromosome Res 4, 147–150 (1996). https://doi.org/10.1007/BF02259708
- fluorescencein situ hybridization
- gene mapping
- transforming growth
- beta 2
- urate oxidase