Chromatographia

, Volume 12, Issue 6, pp 399–404 | Cite as

Application and new possibilities of automation in liquid chromatography

  • F. Erni
  • K. Krummen
  • A. Pellet
Originals

Summary

The different steps of automation of an HPLC system are discussed. With automatic sample injection, a higher through put and a better reproducibility can be achieved than with manual injection.

Peak processing systems are necessary to manage the flood of data. Post run optimization of the integration parameters are possible with a computer system. Data systems provide an enormous flexibility to produce different kinds of reports, summaries and graphs and to perform statistical calculations.

The assay and purity control of a peptide hormone injection (Lys8-Vasopressin) is shown as an example of automated HPLC. Reversed phase gradient elution with UV and reaction detection is used.

Key Words

Automation in LC Data systems for LC Lys8-Vasopressin Quality control 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1979

Authors and Affiliations

  • F. Erni
    • 1
  • K. Krummen
    • 1
  • A. Pellet
    • 1
  1. 1.Analytical Research and Development Pharmaceutical DepartmentSandoz Ltd.BaselSwitzerland

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