Experimental Brain Research

, Volume 88, Issue 2, pp 375–388

Retrograde transport of D-[3H]-aspartate injected into the monkey amygdaloid complex

  • David G. Amaral
  • Ricardo Insausti
Regular Papers

Summary

The possibility that certain of the afferents of the primate amygdaloid complex use an excitatory amino acid transmitter was evaluated by injecting D-[3H]-aspartate into the amygdala of twoMacaca fascicularis monkeys. The distribution of D-[3H]-aspartate labeled neurons was compared with those labeled with the nonselective retrograde tracer WGA-HRP injected at the same location as the isotope. Retrogradely labeled cells of both types were observed in a variety of cortical and subcortical structures and in discrete regions within the amygdala. D-[3H]-aspartate labeled neurons were observed in layers III and V of the frontal, cingulate, insular and temporal cortices. In the hippocampal formation, heavily labeled cells were observed in the CA1 region and in the deep layers of the entorhinal cortex. Of the subcortical afferents, the claustrum and the midbrain peripeduncular nucleus contained the greatest number of D-[3H]-aspartate labeled cells. Subcortical afferents that are not thought to use excitatory amino acids, such as the cholinergic neurons of the basal nucleus of Meynert, did not retrogradely transport the isotope. Within the amygdala, the most conspicuous labeling was in the paralaminar nucleus which forms the rostral and ventral limits of the amygdala. When the D-[3H]-aspartate injection involved the basal nucleus, many labeled cells were also observed in the lateral nucleus. Retrograde transport of D-[3H]-aspartate injected into the amygdala, therefore, appears to demonstrate a subpopulation of inputs that may use an excitatory amino acid transmitter.

Key words

Excitatory amino acids Glutamate Aspartate Cortical afferents Selective retrograde transport Monkey 

Abbreviations

36pl Area

36 (lateral portion of polar region)

36pm Area

36 (medial portion of polar region)

36r Area

36 (rostral portion)

A1

Primary auditory field

ABmg

Accessory basal nucleus of the amygdala (magnocellular portion)

ABpc

Accessory basal nucleus of the amygdala (parvicellular portion)

ac

Anterior commissure

amts

Anterior middle temporal sulcus

as

Arcuate sulcus

B

Basal nucleus of the amygdala

BNM

Basal nucleus of Meynert

CA1

Field CA1 of the Hippocampus

cc

Corpus callosum

C

Central nucleus of the amygdala

CD

Caudate nucleus

Cl

Claustrum

cs

Cingulate sulcus

EC

Entorhinal cortex

G

Gustatory cortex

H

Hippocampus

I

Intercalated nucleus of the amygdala

Ia

Agranular insular cortex

Id

Dysgranular insular cortex

Ig

Granular insular cortex

ils

Inferior limiting sulcus

LA

Lateral auditory field

LGN

Lateral geniculate nucleus

L

Lateral nucleus of the amygdala

los

Lateral orbital sulcus

mos

Medial orbital sulcus

OT

Olfactory tubercle

ot

Optic tract

ots

Occipitotemporal sulcus

PAC

Periamygdaloid cortex

PA

Posterior auditory field

Pi

Parainsular cortex

PIR

Piriform cortex

PL

Paralaminar nucleus of the amygdala

pmts

Posterior middle temporal sulcus

PPN

Peripeduncular nucleus

ps

Principal sulcus

RA

Rostral auditory field

RI

Retroinsular cortex

ros

Rostral sulcus

rs

Rhinal sulcus

sls

Superior limiting sulcus

SN

Substantia nigra

sts

Superior temporal sulcus

TA Area

TA of Von Bonin and Bailey

TE Area

TE of Von Bonin and Bailey

TEO Area

TEO of Von Bonin and Bailey

TF Area

TF of Von Bonin and Bailey

TH Area

TH of Von Bonin and Bailey

V

Lateral ventricle

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • David G. Amaral
    • 1
  • Ricardo Insausti
    • 2
  1. 1.The Salk InstituteSan DiegoUSA
  2. 2.Department of AnatomyUniversity of NavarraPamplonaSpain

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