Physico-chemical modelling of solute retention in reversed-phase HPLC with methanol-water mobile phase
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Summary
A relationship is derived for the retention parameter (i. e. the retardation coefficient, RF of a solute chromatographed in reversed-phase HPLC using methanol-water mixtures as the mobile phase. In our retention model major importance is attributed to the self- and mixed association of the mobile phase molecules, and the quantification of this effect represents a crucial part of our approach. The physico-chemical correctness of the derived relationship was checked with help of measured data and a remarkable agreement was found between the theory and the experiment.
Key Words
Reversed-phase HPLC Binary mobile phases Retention modelPreview
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