Chromatographia

, Volume 27, Issue 11–12, pp 601–604 | Cite as

Purification of human chorionic gonadotropin hormone by anion-exchange high-performance liquid chromatography

  • I. Fellegvári
  • K. Valkó
  • G. Váradi
  • P. Bauer
  • M. Kramer
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Summary

A high-performance liquid chromatography (HPLC) method was developed for the purification of 50mg crude human chorionic gonadotropin (HCG) hormone sample in one chromatographic run on a 250×10 mm SERVA DEAE Poliol-Si-500 column. During the 60-minute linear gradient of 0.05 M sodium sulfate, 0.05 M sodium hydrogen sulfate and 0.05% (v/v) acetonitrile in buffer “B” complete separation was accomplished in 40 min. Identity and purity of the fractions were checked by SDS electrophoretic method. The eluted HCG fractions were dialyzed for 24 hours to remove the buffer salts and liophilized. The activity of the hormone did not decreased significantly during the purification procedure.

Key Words

Column liquid chromatography Anion exchange separation Preparative separations Human chorionic gonadotropin hormone 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • I. Fellegvári
    • 1
  • K. Valkó
    • 1
  • G. Váradi
    • 2
  • P. Bauer
    • 2
  • M. Kramer
    • 3
  1. 1.Central Research Institute for ChemistryHungarian Academy of SciencesBudapestHungary
  2. 2.Department of BiochemistrySemmelweis University of MedicineBudapestHungary
  3. 3.Vepex Ltd.BudapestHungary

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