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Lamp-1 is upregulated in human glioblastoma cell lines induced to undergo apoptosis

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Abstract

Lysosome-associated membrane protein (LAMP)-1, one of the major protein components of the lysosomal membrane, is upregulated in the human glioblastoma cell lines, U-373 MG and LN-Z308, which undergo cisplatin-induced apoptosis. These human brain tumor cell lines demonstrated apoptosis in response to cisplatin/nifedipine treatment. Both cell lines demonstrated an apoptotic response by more than one criterion. Apoptosis was demonstrated by DNA fragmentation techniques such as DNA laddering, ApopTag® in situ labeling, and an ELISA-based method of detecting liberated oligosomes. These cells also had characteristic morphologic changes and upregulation of bax consistent with apoptosis. LAMP-1 expression at the protein and mRNA level was examined and found to increase with cisplatin/nifedipine treatment. LAMP-1 expression was examined using indirect immunofluorescent staining, Northern blot analysis and Western blot analysis. The finding of an augmentation of LAMP-1 in these cells induced to die is enigmatic. These findings raise the possibility of LAMP-1 involvement in the apoptotic process.

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Chen, J.W., Madamanchi, N., Madamanchi, N.R. et al. Lamp-1 is upregulated in human glioblastoma cell lines induced to undergo apoptosis. J Biomed Sci 8, 365–374 (2001). https://doi.org/10.1007/BF02258379

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Key Words

  • Apoptosis
  • Brain tumor
  • Cisplatin
  • LAMP
  • Lysosome