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Regulation of fibronectin by platelet-derived growth factors in cultured rat thoracic aortic smooth muscle cells

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Induction of Fibronectin (FN) gene expression by platelet-derived growth factor (PDGF) isoforms in rat thoracic aortic smooth muscle cells (SMC) was examined. PDGF-BB enhances FN levels in SMC cultures in a time- and concentration-response fashion. PDGF-AA and PDGF-AB show no effect on FN levels. The effects of insulin and insulin-like growth factor-I (IGF-I) on PDGF-BB-induced FN levels were examined. No additivity of FN levels is observed between PDGF-BB and insulin and/or IGF-I. Experiments also show that PDGF-BB enhances FN mRNA levels, implying that acquisition of additional FN mRNA units accounts for the increase in FN levels. Induction of FN and FN mRNA levels by PDGF-BB could be one of the initial events in vascular SMC proliferation and extracellular matrix expansion, leading to atherosclerosis and hypertension.

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Lo, C., Tamaroglio, T. & Zhang, J. Regulation of fibronectin by platelet-derived growth factors in cultured rat thoracic aortic smooth muscle cells. J Biomed Sci 2, 63–69 (1995).

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Key Words

  • Platelet-derived growth factors
  • Fibronectin
  • Vascular smooth muscle cells
  • Insulin
  • Insulin-like growth factor-I
  • Fibronectin mRNA