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Short-term ascorbic acid deficiency induced oxidative stress in the retinas of young guinea pigs

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We examined whether short-term ascorbic acid deficiency induces oxidative stress in the retinas of young guinea pigs. Four-week-old guinea pigs were given a scorbutic diet (20 g/animal/day) with and without adequate ascorbic acid (400 mg/animal/day) in drinking water for 3 weeks. The serum concentrations of the reduced form of ascorbic acid and the oxidized form of ascorbic acid in the deficient group were 14.1 and 4.1%, respectively, of those in the adequate group. The retinal contents of the reduced form of ascorbic acid and the oxidized form of ascorbic acid in the deficient group were 6.4 and 27.3%, respectively, of those in the adequate group. The retinal content of thiobarbituric acid-reactive substances, an index of lipid peroxidation, was 1.9-fold higher in the deficient group than in the adequate group. Retinal reduced glutathione and vitamin E contents in the deficient group were 70.1 and 69.4%, respectively, of those in the adequate group. This ascorbic acid deficiency did not affect serum thiobarbituric acid-reactive substances and reduced glutathione concentrations but increased serum vitamin E concentration. These results indicate that short-term ascorbic acid deficiency induces oxidative stress in the retinas of young guinea pigs without disrupting systemic antioxidant status.

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Ohta, Y., Okubo, T., Niwa, T. et al. Short-term ascorbic acid deficiency induced oxidative stress in the retinas of young guinea pigs. J Biomed Sci 11, 172–178 (2004).

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Key Words

  • Ascorbic acid
  • Reduced glutathione
  • Vitamin E
  • Lipid perioxide
  • Oxidative stress
  • Ascorbic acid deficiency (young guinea pigs)
  • Retina